LOCUS Exported 12593 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of IGF1R R996W. ACCESSION . VERSION . KEYWORDS pHAGE-IGF1R-R996W SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12593) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 12593) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12593 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..1179 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 232..1170 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1127..1147 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1199..1223 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(5331..5355) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 5363..5381 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(5406..5425) /label=T7 /note="T7 promoter, forward primer" promoter complement(5407..5425) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(5431..5451) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 5505..5546 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 5567..6134 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 5584..6136 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5728..5745) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5955..5974 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 6148..6747 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(6148..6167) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6644..6664 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 6761..7349 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6814..6834) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7232..7243) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7424..7657 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7776..7794) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7862..7966 /gene="bla" /label=AmpR promoter CDS 7967..8827 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8185..8204) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8998..9586 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9487..9506 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9740..9757 /label=L4440 /note="L4440 vector, forward primer" primer_bind 9849..9868 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 9997..10018 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 10033..10063 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10071..10087 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 10076..10098 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 10095..10111 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 10095..10111 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 10161..10180 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 10372..11005 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 11052..11177 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 11674..11907 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 12092..12136 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 12429..12546 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg 61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt 121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca 181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc 241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt 301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa gcggccctgc agatatcaac 1201 aagtttgtac aaaaaagcag gcaccatgaa gtctggctcc ggaggagggt ccccgacctc 1261 gctgtggggg ctcctgtttc tctccgccgc gctctcgctc tggccgacga gtggagaaat 1321 ctgcgggcca ggcatcgaca tccgcaacga ctatcagcag ctgaagcgcc tggagaactg 1381 cacggtgatc gagggctacc tccacatcct gctcatctcc aaggccgagg actaccgcag 1441 ctaccgcttc cccaagctca cggtcattac cgagtacttg ctgctgttcc gagtggctgg 1501 cctcgagagc ctcggagacc tcttccccaa cctcacggtc atccgcggct ggaaactctt 1561 ctacaactac gccctggtca tcttcgagat gaccaatctc aaggatattg ggctttacaa 1621 cctgaggaac attactcggg gggccatcag gattgagaaa aatgctgacc tctgttacct 1681 ctccactgtg gactggtccc tgatcctgga tgcggtgtcc aataactaca ttgtggggaa 1741 taagccccca aaggaatgtg gggacctgtg tccagggacc atggaggaga agccgatgtg 1801 tgagaagacc accatcaaca atgagtacaa ctaccgctgc tggaccacaa accgctgcca 1861 gaaaatgtgc ccaagcacgt gtgggaagcg ggcgtgcacc gagaacaatg agtgctgcca 1921 ccccgagtgc ctgggcagct gcagcgcgcc tgacaacgac acggcctgtg tagcttgccg 1981 ccactactac tatgccggtg tctgtgtgcc tgcctgcccg cccaacacct acaggtttga 2041 gggctggcgc tgtgtggacc gtgacttctg cgccaacatc ctcagcgccg agagcagcga 2101 ctccgagggg tttgtgatcc acgacggcga gtgcatgcag gagtgcccct cgggcttcat 2161 ccgcaacggc agccagagca tgtactgcat cccttgtgaa ggtccttgcc cgaaggtctg 2221 tgaggaagaa aagaaaacaa agaccattga ttctgttact tctgctcaga tgctccaagg 2281 atgcaccatc ttcaagggca atttgctcat taacatccga cgggggaata acattgcttc 2341 agagctggag aacttcatgg ggctcatcga ggtggtgacg ggctacgtga agatccgcca 2401 ttctcatgcc ttggtctcct tgtccttcct aaaaaacctt cgcctcatcc taggagagga 2461 gcagctagaa gggaattact ccttctacgt cctcgacaac cagaacttgc agcaactgtg 2521 ggactgggac caccgcaacc tgaccatcaa agcagggaaa atgtactttg ctttcaatcc 2581 caaattatgt gtttccgaaa tttaccgcat ggaggaagtg acggggacta aagggcgcca 2641 aagcaaaggg gacataaaca ccaggaacaa cggggagaga gcctcctgtg aaagtgacgt 2701 cctgcatttc acctccacca ccacgtcgaa gaatcgcatc atcataacct ggcaccggta 2761 ccggccccct gactacaggg atctcatcag cttcaccgtt tactacaagg aagcaccctt 2821 taagaatgtc acagagtatg atgggcagga tgcctgcggc tccaacagct ggaacatggt 2881 ggacgtggac ctcccgccca acaaggacgt ggagcccggc atcttactac atgggctgaa 2941 gccctggact cagtacgccg tttacgtcaa ggctgtgacc ctcaccatgg tggagaacga 3001 ccatatccgt ggggccaaga gtgagatctt gtacattcgc accaatgctt cagttccttc 3061 cattcccttg gacgttcttt cagcatcgaa ctcctcttct cagttaatcg tgaagtggaa 3121 ccctccctct ctgcccaacg gcaacctgag ttactacatt gtgcgctggc agcggcagcc 3181 tcaggacggc tacctttacc ggcacaatta ctgctccaaa gacaaaatcc ccatcaggaa 3241 gtatgccgac ggcaccatcg acattgagga ggtcacagag aaccccaaga ctgaggtgtg 3301 tggtggggag aaagggcctt gctgcgcctg ccccaaaact gaagccgaga agcaggccga 3361 gaaggaggag gctgaatacc gcaaagtctt tgagaatttc ctgcacaact ccatcttcgt 3421 gcccagacct gaaaggaagc ggagagatgt catgcaagtg gccaacacca ccatgtccag 3481 ccgaagcagg aacaccacgg ccgcagacac ctacaacatc accgacccgg aagagctgga 3541 gacagagtac cctttctttg agagcagagt ggataacaag gagagaactg tcatttctaa 3601 ccttcggcct ttcacattgt accgcatcga tatccacagc tgcaaccacg aggctgagaa 3661 gctgggctgc agcgcctcca acttcgtctt tgcaaggact atgcccgcag aaggagcaga 3721 tgacattcct gggccagtga cctgggagcc aaggcctgaa aactccatct ttttaaagtg 3781 gccggaacct gagaatccca atggattgat tctaatgtat gaaataaaat acggatcaca 3841 agttgaggat cagcgagaat gtgtgtccag acaggaatac aggaagtatg gaggggccaa 3901 gctaaaccgg ctaaacccgg ggaactacac agcccggatt caggccacat ctctctctgg 3961 gaatgggtcg tggacagatc ctgtgttctt ctatgtccag gccaaaacag gatatgaaaa 4021 cttcatccat ctgatcatcg ctctgcccgt cgctgtcctg ttgatcgtgg gagggttggt 4081 gattatgctg tacgtcttcc atagaaagag aaataacagc aggctgggga atggagtgct 4141 gtatgcctct gtgaacccgg agtacttcag cgctgctgat gtgtacgttc ctgatgagtg 4201 ggaggtggct tgggagaaga tcaccatgag ccgggaactt gggcaggggt cgtttgggat 4261 ggtctatgaa ggagttgcca agggtgtggt gaaagatgaa cctgaaacca gagtggccat 4321 taaaacagtg aacgaggccg caagcatgcg tgagaggatt gagtttctca acgaagcttc 4381 tgtgatgaag gagttcaatt gtcaccatgt ggtgcgattg ctgggtgtgg tgtcccaagg 4441 ccagccaaca ctggtcatca tggaactgat gacacggggc gatctcaaaa gttatctccg 4501 gtctctgagg ccagaaatgg agaataatcc agtcctagca cctccaagcc tgagcaagat 4561 gattcagatg gccggagaga ttgcagacgg catggcatac ctcaacgcca ataagttcgt 4621 ccacagagac cttgctgccc ggaattgcat ggtagccgaa gatttcacag tcaaaatcgg 4681 agattttggt atgacgcgag atatctatga gacagactat taccggaaag gagggaaagg 4741 gctgctgccc gtgcgctgga tgtctcctga gtccctcaag gatggagtct tcaccactta 4801 ctcggacgtc tggtccttcg gggtcgtcct ctgggagatc gccacactgg ccgagcagcc 4861 ctaccagggc ttgtccaacg agcaagtcct tcgcttcgtc atggagggcg gccttctgga 4921 caagccagac aactgtcctg acatgctgtt tgaactgatg cgcatgtgct ggcagtataa 4981 ccccaagatg aggccttcct tcctggagat catcagcagc atcaaagagg agatggagcc 5041 tggcttccgg gaggtctcct tctactacag cgaggagaac aagctgcccg agccggagga 5101 gctggacctg gagccagaga acatggagag cgtccccctg gacccctcgg cctcctcgtc 5161 ctccctgcca ctgcccgaca gacactcagg acacaaggcc gagaacggcc ccggccctgg 5221 ggtgctggtc ctccgcgcca gcttcgacga gagacagcct tacgcccaca tgaacggggg 5281 ccgcaagaac gagcgggcct tgccgctgcc ccagtcttcg acctgctaga acccagcttt 5341 cttgtacaaa gtggtgatat ccaattaacc ctcactaaag gttgtatgtg agtttagatt 5401 aatgacccta tagtgagtcg tattaccacc caacttttct atacaaagtg gttgatatcc 5461 agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta 5521 accctctcct cggtctcgat tctacgcgta ccggttagta atgagatccc tccccccccc 5581 ntaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat 5641 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct 5701 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg 5761 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc 5821 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg 5881 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg 5941 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga 6001 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt 6061 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa 6121 aaacacgatg ataatatggc cacacatatg accgagtaca agcccacggt gcgcctcgcc 6181 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 6241 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 6301 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 6361 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 6421 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 6481 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 6541 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 6601 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 6661 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 6721 tgcatgaccc gcaagcccgg tgcctaaatc gatagatcct aatcaacctc tggattacaa 6781 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 6841 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 6901 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 6961 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 7021 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 7081 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 7141 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat 7201 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 7261 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag 7321 tcggatctcc ctttgggccg cctccccgcc tgagatcctt taagaccaat gacttacaag 7381 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac 7441 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc 7501 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 7561 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 7621 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt 7681 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc cgggttaatt 7741 aaggaaaggg ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata 7801 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt 7861 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 7921 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 7981 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 8041 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 8101 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 8161 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg 8221 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 8281 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 8341 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 8401 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 8461 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc 8521 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 8581 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 8641 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 8701 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 8761 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 8821 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 8881 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 8941 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 9001 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 9061 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 9121 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa 9181 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 9241 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 9301 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 9361 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 9421 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 9481 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 9541 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 9601 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 9661 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg 9721 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg 9781 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcaagctca tggctgacta 9841 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 9901 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt 9961 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 10021 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 10081 ataacaattt cacacaggaa acagctatga catgattacg aatttcacaa ataaagcatt 10141 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 10201 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat 10261 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt 10321 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc 10381 taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct 10441 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg 10501 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag 10561 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag 10621 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc 10681 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact 10741 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat 10801 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag 10861 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt 10921 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt 10981 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga 11041 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg 11101 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag 11161 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc 11221 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg 11281 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa 11341 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata 11401 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag 11461 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg 11521 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata 11581 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag 11641 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg 11701 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt 11761 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca 11821 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga 11881 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg 11941 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa 12001 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc 12061 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga 12121 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt 12181 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat 12241 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc 12301 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag 12361 atccattcga ttagtgaacg gatctcgacg gtatcgccga attcacaaat ggcagtattc 12421 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta 12481 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa 12541 aattttcggg tttattacag ggacagcaga gatccagttt ggactagtcg tga //