LOCUS Exported 4633 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION pAAV plasmid expressing 3xFLAG under the hSyn promoter. ACCESSION . VERSION . KEYWORDS pAAV-hSyn-3xFLAG-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4633) AUTHORS Candemir E, Kollert L, Weissflog L, Geis M, Muller A, Post AM, O'Leary A, Harro J, Reif A, Freudenberg F TITLE Interaction of NOS1AP with the NOS-I PDZ domain: Implications for schizophrenia-related alterations in dendritic morphology. JOURNAL Eur Neuropsychopharmacol. 2016 Apr;26(4):741-55. doi: 10.1016/j.euroneuro.2016.01.008. Epub 2016 Jan 28. PUBMED 26861996 REFERENCE 2 (bases 1 to 4633) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4633 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" promoter 175..622 /label=hSyn promoter /note="human synapsin I promoter; confers neuron-specific expression (Kugler et al., 2003)" regulatory 660..669 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 675..740 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" misc_feature 760..1348 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(813..833) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(1231..1242) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 1380..1856 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(1579..1598) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 1896..2036 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 1896..2025 /label=AAV2 ITR rep_origin 2111..2566 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2198..2217) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2408..2429 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(2583..2602) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 2702..2724 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(2762..2780) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 2848..2952 /gene="bla" /label=AmpR promoter CDS 2953..3813 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(3171..3190) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 3984..4572 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4473..4492 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgtgtgtct agactgcaga gggccctgcg tatgagtgca 181 agtgggtttt aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga 241 cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg 301 gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt 361 gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac 421 gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc 481 cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca 541 tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt 601 cgtgtcgtgc ctgagagcgc agtcgagaag gtaccggatc ctctagagtc gacactagtg 661 ccaccatgga attcgactac aaagaccatg acggtgatta taaagatcat gatattgatt 721 acaaggatga cgatgacaag ctagcataag cttatcgata atcaacctct ggattacaaa 781 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac 841 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc 901 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt 961 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc 1021 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc 1081 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg 1141 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctatgttgc cacctggatt 1201 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc 1261 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt 1321 cggatctccc tttgggccgc ctccccgcat cgataccgag cgctgctcga gagatctacg 1381 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag 1441 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct 1501 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa 1561 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc 1621 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt 1681 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg 1741 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt 1801 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga 1861 ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc 1921 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg 1981 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg 2041 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag 2101 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 2161 agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc 2221 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 2281 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca 2341 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 2401 tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc gggctattct 2461 tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa 2521 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc 2581 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct 2641 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc 2701 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag 2761 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg 2821 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 2881 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 2941 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca 3001 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat 3061 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 3121 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 3181 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct 3241 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 3301 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 3361 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 3421 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 3481 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta 3541 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 3601 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 3661 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 3721 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 3781 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata 3841 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 3901 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 3961 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 4021 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 4081 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 4141 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 4201 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 4261 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 4321 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 4381 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 4441 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 4501 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 4561 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 4621 tttgctcaca tgt //