LOCUS       Exported                5971 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP-S fusion protein 
            under the hSyn promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-hSyn-mCherry.3xFLAG.NOS1AP-S-WPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Candemir E, Kollert L, Weissflog L, Geis M, Muller A, Post AM, 
            O'Leary A, Harro J, Reif A, Freudenberg F
  TITLE     Interaction of NOS1AP with the NOS-I PDZ domain: Implications for 
            schizophrenia-related alterations in dendritic morphology.
  JOURNAL   Eur Neuropsychopharmacol. 2016 Apr;26(4):741-55. doi: 
            10.1016/j.euroneuro.2016.01.008. Epub 2016 Jan 28.
  PUBMED    26861996
REFERENCE   2  (bases 1 to 5971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        175..622
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      654..663
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             660..1367
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(805..823)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1073..1092
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1374..1439
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    2098..2686
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2151..2171)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2569..2580)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2718..3194
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2917..2936)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3234..3374
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3234..3363
                     /label=AAV2 ITR
     rep_origin      3449..3904
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3536..3555)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3746..3767
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3921..3940)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4040..4062
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4100..4118)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4186..4290
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4291..5151
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4509..4528)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5322..5910
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5811..5830
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtgtct agactgcaga gggccctgcg tatgagtgca
      181 agtgggtttt aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga
      241 cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg
      301 gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt
      361 gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac
      421 gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc
      481 cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca
      541 tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt
      601 cgtgtcgtgc ctgagagcgc agtcgagaag gtaccggatc ctctagagtc gacgccacca
      661 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
      721 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
      781 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
      841 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
      901 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
      961 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
     1021 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
     1081 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
     1141 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
     1201 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
     1261 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
     1321 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaaggaa ttcgactaca
     1381 aagaccatga cggtgattat aaagatcatg atattgatta caaggatgac gatgacaagc
     1441 tagctatgtc cctctcttct ctctgtcctg tcttctctgc cgctgcctct tctctgcagg
     1501 tacacttact gaaggatcag ttggctgctg aggctgcggc gcggctggag gcccaggcac
     1561 gagtgcacca gctcctgctg cagaacaaag acatgctcca gcacatctct ctgctggtca
     1621 agcaggtgca ggagctggag ctgaagctgt caggacagaa caccatgggc tcccaggaca
     1681 gcttgctgga gatcaccttc cgttcaggtg ccctgcctgt gctctgtgaa tccaccaccc
     1741 ctaagccaga ggacctacac tcaccactgc tgggcgctgg cttggctgac tttgcccacc
     1801 cagcgggcag ccctttaggt aggcatgact gcttggtgaa gctggagtgc tttcgcttcc
     1861 tcccacctga ggatacccag ccgatgatgg cacagggtga gccgctccta ggtggcctgg
     1921 agctcatcaa gttccgagag tcgggcatcg cctctgagta tgagtccaac acagacgaaa
     1981 gcgaggagcg tgactcatgg tcacaggaag agctgccacg cctgctcaat gtcttacagc
     2041 ggcaggagtt gggtgacagt ttggatgatg agatcgccgt gtagtaagct tatcgataat
     2101 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     2161 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     2221 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     2281 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     2341 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     2401 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     2461 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     2521 tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     2581 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     2641 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgagcg
     2701 ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct ctcctggccc
     2761 tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt
     2821 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg
     2881 ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg
     2941 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc
     3001 ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt
     3061 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc
     3121 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc
     3181 ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc
     3241 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     3301 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     3361 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     3421 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     3481 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct
     3541 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     3601 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     3661 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     3721 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     3781 tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta
     3841 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     3901 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     3961 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     4021 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     4081 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     4141 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4201 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4261 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     4321 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     4381 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     4441 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     4501 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     4561 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4621 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4681 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     4741 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     4801 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     4861 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     4921 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     4981 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     5041 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5101 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5161 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5221 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     5281 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5341 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5401 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5461 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5521 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5581 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5641 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5701 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5761 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5821 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5881 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     5941 ctggcctttt gctggccttt tgctcacatg t
//
