LOCUS Exported 5032 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION pAAV plasmid expressing an NOS-IN133.3xFLAG fusion protein under the hSyn promoter. ACCESSION . VERSION . KEYWORDS pAAV-hSyn-NOS-IN133.3xFLAG-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5032) AUTHORS Candemir E, Kollert L, Weissflog L, Geis M, Muller A, Post AM, O'Leary A, Harro J, Reif A, Freudenberg F TITLE Interaction of NOS1AP with the NOS-I PDZ domain: Implications for schizophrenia-related alterations in dendritic morphology. JOURNAL Eur Neuropsychopharmacol. 2016 Apr;26(4):741-55. doi: 10.1016/j.euroneuro.2016.01.008. Epub 2016 Jan 28. PUBMED 26861996 REFERENCE 2 (bases 1 to 5032) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5032 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" promoter 175..622 /label=hSyn promoter /note="human synapsin I promoter; confers neuron-specific expression (Kugler et al., 2003)" regulatory 660..669 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1074..1139 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" misc_feature 1159..1747 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(1212..1232) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(1630..1641) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 1779..2255 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(1978..1997) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 2295..2435 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 2295..2424 /label=AAV2 ITR rep_origin 2510..2965 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2597..2616) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2807..2828 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(2982..3001) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 3101..3123 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(3161..3179) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 3247..3351 /gene="bla" /label=AmpR promoter CDS 3352..4212 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(3570..3589) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 4383..4971 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4872..4891 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgtgtgtct agactgcaga gggccctgcg tatgagtgca 181 agtgggtttt aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga 241 cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg 301 gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt 361 gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac 421 gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc 481 cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca 541 tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt 601 cgtgtcgtgc ctgagagcgc agtcgagaag gtaccggatc ctctagagtc gacactagtg 661 ccaccatgga agagcacacg tttggggtcc agcagatcca acccaacgtc atttctgtcc 721 gtctcttcaa acgcaaagtg ggaggtctgg gcttcctggt gaaggaacgg gtcagcaagc 781 ccccggtgat catctcagat ctgatccgag gaggtgccgc ggagcagagc ggccttatcc 841 aagccggcga catcattctc gccgtcaatg atcggcccct ggtagacctc agctatgaca 901 gcgctctgga agttctcagg ggcattgcct ctgagaccca tgtggtcctc attctgagag 961 gccctgaggg ctttactaca cacctggaga ccaccttcac aggggatgga acccccaaga 1021 ccatccgtgt gacccagccc ctggggacac ccaccaaagc tgtcgatgaa ttcgactaca 1081 aagaccatga cggtgattat aaagatcatg atattgatta caaggatgac gatgacaagc 1141 tagcataagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt 1201 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 1261 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 1321 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 1381 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 1441 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 1501 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg 1561 tcctttcctt ggctgctcgc ctatgttgcc acctggattc tgcgcgggac gtccttctgc 1621 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 1681 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc 1741 tccccgcatc gataccgagc gctgctcgag agatctacgg gtggcatccc tgtgacccct 1801 ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa 1861 taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga 1921 ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc tgcggggtct 1981 attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct 2041 gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga 2101 ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc 2161 tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat 2221 tacaggcgtg aaccactgct cccttccctg tccttctgat tttgtaggta accacgtgcg 2281 gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg 2341 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc 2401 ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct 2461 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt 2521 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 2581 agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 2641 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 2701 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga 2761 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 2821 caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg 2881 ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt 2941 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc 3001 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt 3061 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag 3121 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt 3181 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga 3241 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 3301 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 3361 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 3421 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 3481 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 3541 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac 3601 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 3661 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 3721 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 3781 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 3841 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 3901 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 3961 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 4021 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 4081 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 4141 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 4201 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 4261 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 4321 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 4381 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 4441 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 4501 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 4561 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 4621 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 4681 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 4741 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 4801 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 4861 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 4921 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 4981 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gt //