LOCUS Exported 6844 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP fusion protein under the hSyn promoter. ACCESSION . VERSION . KEYWORDS pAAV-hSyn-mCherry.3xFLAG.NOS1AP-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6844) AUTHORS Candemir E, Kollert L, Weissflog L, Geis M, Muller A, Post AM, O'Leary A, Harro J, Reif A, Freudenberg F TITLE Interaction of NOS1AP with the NOS-I PDZ domain: Implications for schizophrenia-related alterations in dendritic morphology. JOURNAL Eur Neuropsychopharmacol. 2016 Apr;26(4):741-55. doi: 10.1016/j.euroneuro.2016.01.008. Epub 2016 Jan 28. PUBMED 26861996 REFERENCE 2 (bases 1 to 6844) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6844 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" promoter 175..622 /label=hSyn promoter /note="human synapsin I promoter; confers neuron-specific expression (Kugler et al., 2003)" regulatory 654..663 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 660..1367 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(805..823) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 1073..1092 /label=mCherry-F /note="mCherry, forward primer" CDS 1374..1439 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" misc_feature 2971..3559 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3024..3044) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3442..3453) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 3591..4067 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(3790..3809) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 4107..4247 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 4107..4236 /label=AAV2 ITR rep_origin 4322..4777 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4409..4428) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4619..4640 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4794..4813) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4913..4935 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4973..4991) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 5059..5163 /gene="bla" /label=AmpR promoter CDS 5164..6024 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5382..5401) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6195..6783 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6684..6703 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgtgtgtct agactgcaga gggccctgcg tatgagtgca 181 agtgggtttt aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga 241 cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg 301 gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt 361 gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac 421 gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc 481 cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca 541 tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt 601 cgtgtcgtgc ctgagagcgc agtcgagaag gtaccggatc ctctagagtc gacgccacca 661 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg 721 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc 781 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct 841 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc 901 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg 961 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg 1021 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa 1081 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg 1141 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg 1201 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca 1261 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac 1321 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaaggaa ttcgactaca 1381 aagaccatga cggtgattat aaagatcatg atattgatta caaggatgac gatgacaagc 1441 tagctatgcc cagcaaaacc aagtacaacc ttgtggacga tgggcacgat ctacggatcc 1501 ctttgcacaa cgaggacgcc ttccagcacg gcatctcttt tgaggccaag tacgtgggaa 1561 gcctggatgt gcccagaccc aacagcaggg ttgagatcgt ggctgccatg cgcagaatcc 1621 ggtatgagtt taaagccaag aacatcaaga agaagaaagt aagcatcatg gtctctgtgg 1681 acggtgtcaa ggtgattctg aagaagaaga aaaagaaaaa ggagtggacg tgggatgaga 1741 gcaagatgct ggtgatgcag gaccctatct acaggatctt ctatgtctct catgactccc 1801 aagacttgaa aatcttcagc tatattgcca gagatggtgc cagcaatatc ttcagatgta 1861 acgtctttaa atccaaaaag aagagtcaag ctatgagaat cgtgcggaca gtgggacagg 1921 ccttcgaagt ctgccacaag ctgagcctgc agcacacaca gcagaatgca gatggccagg 1981 aagatgggga gagcgagagg aacagtgatg gctcaggaga cccaggccgg cagctcacgg 2041 gagctgagag ggtctccaca gccgctgcag aggagacaga cattgacgcc gtggaggtcc 2101 cactccctgg gaatgacatt ctggaattca gccgaggtgt gactgatctg gatgccgtag 2161 ggaaggatgg aggctcccac atagactcaa cggtctcacc ccaccctcag gagcccatgc 2221 tgacagcctc ccctcgaatg ctgctccctt cctcttcctc gaagccgcca ggcttgggca 2281 cggggacgcc cctgtccact caccaccaga tgcagctcct ccagcagctc ctccagcagc 2341 agcaacaaca gacacaagtg gctgtggccc aggtacactt actgaaggat cagttggctg 2401 ctgaggctgc ggcgcggctg gaggcccagg cacgagtgca ccagctcctg ctgcagaaca 2461 aagacatgct ccagcacatc tctctgctgg tcaagcaggt gcaggagctg gagctgaagc 2521 tgtcaggaca gaacaccatg ggctcccagg acagcttgct ggagatcacc ttccgttcag 2581 gtgccctgcc tgtgctctgt gaatccacca cccctaagcc agaggaccta cactcaccac 2641 tgctgggcgc tggcttggct gactttgccc acccagcggg cagcccttta ggtaggcatg 2701 actgcttggt gaagctggag tgctttcgct tcctcccacc tgaggatacc cagccgatga 2761 tggcacaggg tgagccgctc ctaggtggcc tggagctcat caagttccga gagtcgggca 2821 tcgcctctga gtatgagtcc aacacagacg aaagcgagga gcgtgactca tggtcacagg 2881 aagagctgcc acgcctgctc aatgtcttac agcggcagga gttgggtgac agtttggatg 2941 atgagatcgc cgtgtagtaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa 3001 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 3061 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 3121 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 3181 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 3241 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 3301 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 3361 gggaaatcat cgtcctttcc ttggctgctc gcctatgttg ccacctggat tctgcgcggg 3421 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 3481 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc 3541 ctttgggccg cctccccgca tcgataccga gcgctgctcg agagatctac gggtggcatc 3601 cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca 3661 gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata 3721 ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg 3781 cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa 3841 tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc 3901 aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca 3961 tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca 4021 aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg attttgtagg 4081 taaccacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc 4141 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt 4201 tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg 4261 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag 4321 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 4381 gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 4441 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 4501 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg 4561 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 4621 ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta 4681 taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt 4741 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc 4801 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc 4861 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc 4921 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg 4981 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc 5041 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat 5101 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 5161 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt 5221 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt 5281 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc 5341 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta 5401 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac 5461 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa 5521 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg 5581 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc 5641 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg 5701 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta 5761 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg 5821 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg 5881 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc 5941 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt 6001 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt 6061 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 6121 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 6181 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 6241 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 6301 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag 6361 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 6421 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 6481 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 6541 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 6601 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 6661 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 6721 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 6781 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 6841 atgt //