LOCUS Exported 9791 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of MAP2K1 K57N. ACCESSION . VERSION . KEYWORDS pHAGE-MAP2K1-K57N SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9791) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 9791) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9791 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(4631..4655) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 4663..4681 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(4706..4725) /label=T7 /note="T7 promoter, forward primer" promoter complement(4707..4725) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(4731..4751) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 4805..4846 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 4867..5434 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 4884..5436 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5028..5045) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5255..5274 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5448..6167 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 5448..6164 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(5493..5514) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5754..5773) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6101..6122 /label=EGFP-C /note="EGFP, forward primer" misc_feature 6181..6769 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6234..6254) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6652..6663) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6844..7077 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7196..7214) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7282..7386 /gene="bla" /label=AmpR promoter CDS 7387..8247 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7605..7624) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8418..9006 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8907..8926 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9160..9177 /label=L4440 /note="L4440 vector, forward primer" primer_bind 9269..9288 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 9417..9438 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9453..9483 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9491..9507 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9496..9518 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9515..9531 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9515..9531 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 9581..9600 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcaccatg cccaagaaga agccgacgcc catccagctg 3481 aacccggccc ccgacggctc tgcagttaac gggaccagct ctgcggagac caacttggag 3541 gccttgcaga agaagctgga ggagctagag cttgatgagc agcagcgaaa gcgccttgag 3601 gcctttctta cccagaatca gaaggtggga gaactgaagg atgacgactt tgagaagatc 3661 agtgagctgg gggctggcaa tggcggtgtg gtgttcaagg tctcccacaa gccttctggc 3721 ctggtcatgg ccagaaagct aattcatctg gagatcaaac ccgcaatccg gaaccagatc 3781 ataagggagc tgcaggttct gcatgagtgc aactctccgt acatcgtggg cttctatggt 3841 gcgttctaca gcgatggcga gatcagtatc tgcatggagc acatggatgg aggttctctg 3901 gatcaagtcc tgaagaaagc tggaagaatt cctgaacaaa ttttaggaaa agttagcatt 3961 gctgtaataa aaggcctgac atatctgagg gagaagcaca agatcatgca cagagatgtc 4021 aagccctcca acatcctagt caactcccgt ggggagatca agctctgtga ctttggggtc 4081 agcgggcagc tcatcgactc catggccaac tccttcgtgg gcacaaggtc ctacatgtcg 4141 ccagaaagac tccaggggac tcattactct gtgcagtcag acatctggag catgggactg 4201 tctctggtag agatggcggt tgggaggtat cccatccctc ctccagatgc caaggagctg 4261 gagctgatgt ttgggtgcca ggtggaagga gatgcggctg agaccccacc caggccaagg 4321 acccccggga ggccccttag ctcatacgga atggacagcc gacctcccat ggcaattttt 4381 gagttgttgg attacatagt caacgagcct cctccaaaac tgcccagtgg agtgttcagt 4441 ctggaatttc aagattttgt gaataaatgc ttaataaaaa accccgcaga gagagcagat 4501 ttgaagcaac tcatggttca tgcttttatc aagagatctg atgctgagga agtggatttt 4561 gcaggttggc tctgctccac catcggcctt aaccagccca gcacaccaac ccatgctgct 4621 ggcgtctaga acccagcttt cttgtacaaa gtggtgatat ccaattaacc ctcactaaag 4681 ggtaagatta gaagttaatg aagaacccta tagtgagtcg tattaccacc caacttttct 4741 atacaaagtg gttgatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt 4801 cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta ccggttagta 4861 atgagatccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg 4921 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg 4981 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg 5041 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca 5101 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct 5161 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca 5221 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa 5281 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg 5341 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg 5401 ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacacatatg gtgagcaagg 5461 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 5521 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 5581 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc 5641 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct 5701 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 5761 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 5821 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 5881 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga 5941 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 6001 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc 6061 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 6121 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaatc gatagatcct 6181 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 6241 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 6301 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 6361 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 6421 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 6481 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 6541 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc 6601 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 6661 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt 6721 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgagatcctt 6781 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg 6841 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt 6901 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 6961 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 7021 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta 7081 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga 7141 gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt cttgaagacg aaagggcctc 7201 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt 7261 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca 7321 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 7381 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc 7441 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg 7501 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt 7561 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta 7621 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat 7681 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga 7741 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca 7801 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact 7861 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc 7921 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact 7981 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt 8041 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt 8101 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt 8161 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 8221 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag 8281 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 8341 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 8401 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 8461 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 8521 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg 8581 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 8641 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 8701 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 8761 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 8821 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 8881 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 8941 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 9001 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 9061 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag 9121 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 9181 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 9241 agcaagctca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 9301 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc 9361 tccccgtggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 9421 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 9481 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg 9541 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 9601 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa 9661 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc 9721 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca 9781 aacttagtag t //