LOCUS       Exported               13319 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Lentiviral expression of MAP3K4 P27_P28insP.
ACCESSION   .
VERSION     .
KEYWORDS    pHAGE-MAP3K4-P27_P28insP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13319)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 13319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13319
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3505..3514
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     RBS             5415..5423
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(8279..8303)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        8311..8329
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8354..8373)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8355..8373)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(8379..8399)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             8453..8494
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    8515..9082
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    8532..9084
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8676..8693)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8903..8922
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9096..9695
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9096..9115)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9592..9612
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9709..10297
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9762..9782)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10180..10191)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10372..10605
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10724..10742)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10810..10914
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10915..11775
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11133..11152)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11946..12534
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12435..12454
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12688..12705
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     12797..12816
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    12945..12966
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12981..13011
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13019..13035
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13024..13046
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13043..13059
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13043..13059
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13109..13128
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcttcatg agagaagccg ctgccgcgct ggtccctcct
     3481 cccgcctttg ccgtcacgcc tgccgccgcc atggaggagc cgccgccacc gccgccgccg
     3541 ccgccaccac cgccaccgga acccgagacc gagtcagaac ccgagtgctg cttggcggcg
     3601 aggcaagagg gcacattggg agattcagct tgcaagagtc ctgaatctga tctagaagac
     3661 ttctccgatg aaacaaatac agagaatctt tatggtacct ctccccccag cacacctcga
     3721 cagatgaaac gcatgtcaac caaacatcag aggaataatg tggggaggcc agccagtcgg
     3781 tctaatttga aagaaaaaat gaatgcacca aatcagcctc cacataaaga cactggaaaa
     3841 acagtggaga atgtggaaga atacagctat aagcaggaga aaaagatccg agcagctctt
     3901 agaacaacag agcgtgatca taaaaaaaat gtacagtgct cattcatgtt agactcagtg
     3961 ggtggatctt tgccaaaaaa atcaattcca gatgtggatc tcaataagcc ttacctcagc
     4021 cttggctgta gcaatgctaa gcttccagta tctgtgccca tgcctatagc cagacctgca
     4081 cgccagactt ctaggactga ctgtccagca gatcgtttaa agttttttga aactttacga
     4141 cttttgctaa agcttacctc agtctcaaag aaaaaagaca gggagcaaag aggacaagaa
     4201 aatacgtctg gtttctggct taaccgatct aacgaactga tctggttaga gctacaagcc
     4261 tggcatgcag gacggacaat taacgaccag gacttctttt tatatacagc ccgtcaagcc
     4321 atcccagata ttattaatga aatccttact ttcaaagtcg actatgggag cttcgccttt
     4381 gttagagata gagctggttt taatggtact tcagtagaag ggcagtgcaa agccactcct
     4441 ggaacaaaga ttgtaggtta ctcaacacat catgagcatc tccaacgcca gagggtctca
     4501 tttgagcagg taaaacggat aatggagctg ctagagtaca tagaagcact ttatccatca
     4561 ttgcaggctc ttcagaagga ctatgaaaaa tatgctgcaa aagacttcca ggacagggtg
     4621 caggcactct gtttgtggtt aaacatcaca aaagacttaa atcagaaatt aaggattatg
     4681 ggcactgttt tgggcatcaa gaatttatca gacattggct ggccagtgtt tgaaatccct
     4741 tcccctcgac catccaaagg taatgagccg gagtatgagg gtgatgacac agaaggagaa
     4801 ttaaaggagt tggaaagtag tacggatgag agtgaagaag aacaaatctc tgatcctagg
     4861 gtaccggaaa tcagacagcc catagataac agcttcgaca tccagtcgcg ggactgcata
     4921 tccaagaagc ttgagaggct cgaatctgag gatgattctc ttggctgggg agcaccagac
     4981 tggagcacag aagcaggctt tagtagacat tgtctgactt ctatttatag accatttgta
     5041 gacaaagcac tgaagcagat ggggttaaga aagttaattt taagacttca caagctaatg
     5101 gatggttcct tgcaaagggc acgtatagca ttggtaaaga acgatcgtcc agtggagttt
     5161 tctgaatttc cagatcccat gtggggttca gattatgtgc agttgtcaag gacaccacct
     5221 tcatctgagg agaaatgcag tgctgtgtcg tgggaggagc tgaaggccat ggatttacct
     5281 tcattcgaac ctgccttcct agttctctgc cgagtccttc tgaatgtcat acatgagtgt
     5341 ctgaagttaa gattggagca gagacctgct ggagaaccat ctctcttgag tattaagcag
     5401 ctggtgagag agtgtaagga ggtcctgaag ggcggcctgc tgatgaagca gtactaccag
     5461 ttcatgctgc aggaggttct ggaggacttg gagaagcccg actgcaacat tgacgctttt
     5521 gaagaggatc tacataaaat gcttatggtg tattttgatt acatgagaag ctggatccaa
     5581 atgctacagc aattacctca agcatcgcat agtttaaaaa atctgttaga agaagaatgg
     5641 aatttcacca aagaaataac tcattacata cggggaggag aagcacaggc cgggaagctt
     5701 ttctgtgaca ttgcaggaat gctgctgaaa tctacaggaa gttttttaga atttggctta
     5761 caggagagct gtgctgaatt ttggactagt gcggatgaca gcagtgcttc cgacgaaatc
     5821 aggaggtctg ttatagagat cagtcgagcc ctgaaggagc tcttccatga agccagagaa
     5881 agggcttcca aagcacttgg atttgctaaa atgttgagaa aggacctgga aatagcagca
     5941 gaattcaggc tttcagcccc agttagagac ctcctggatg ttctgaaatc aaaacagtat
     6001 gtcaaggtgc aaattcctgg gttagaaaac ttgcaaatgt ttgttccaga cactcttgct
     6061 gaggagaaga gtattatttt gcagttactc aatgcagctg caggaaagga ctgttcaaaa
     6121 gattcagatg acgtactcat cgatgcctat ctgcttctga ccaagcacgg tgatcgagcc
     6181 cgtgattcag aggacagctg gggcacctgg gaggcacagc ctgtcaaagt cgtgcctcag
     6241 gtggagactg ttgacaccct gagaagcatg caggtggata atcttttact agttgtcatg
     6301 cagtctgcgc atctcacaat tcagagaaaa gctttccagc agtccattga gggacttatg
     6361 actctgtgcc aggagcagac atccagtcag ccggtcatcg ccaaagcttt gcagcagctg
     6421 aagaatgatg cattggagct atgcaacagg ataagcaatg ccattgaccg cgtggaccac
     6481 atgttcacat cagaatttga tgctgaggtt gatgaatctg aatctgtcac cttgcaacag
     6541 tactaccgag aagcaatgat tcaggggtac aattttggat ttgagtatca taaagaagtt
     6601 gttcgtttga tgtctgggga gtttagacag aagataggag acaaatatat aagctttgcc
     6661 cggaagtgga tgaattatgt cctgactaaa tgtgagagtg gtagaggtac aagacccagg
     6721 tgggcgactc aaggatttga ttttctacaa gcaattgaac ctgcctttat ttcagcttta
     6781 ccagaagatg acttcttgag tttacaagcc ttgatgaatg aatgcattgg ccatgtcata
     6841 ggaaaaccac acagtcctgt tacaggtttg taccttgcca ttcatcggaa cagcccccgt
     6901 cctatgaagg tacctcgatg ccatagtgac cctcctaacc cacacctcat tatccccact
     6961 ccagagggat tcagcactcg gagcatgcct tccgacgcgc ggagccatgg cagccctgct
     7021 gctgctgctg ctgctgctgc tgctgctgtt gctgccagtc ggcccagccc ctctggtggt
     7081 gactctgtgc tgcccaaatc catcagcagt gcccatgata ccaggggttc cagcgttcct
     7141 gaaaatgatc gattggcttc catagctgct gaattgcagt ttaggtccct gagtcgtcac
     7201 tcaagcccca cggaggagcg agatgaacca gcatatccaa gaggagattc aagtgggtcc
     7261 acaagaagaa gttgggaact tcggacacta atcagccaga gtaaagatac tgcttctaaa
     7321 ctaggaccca tagaagctat ccagaagtca gtccgattgt ttgaagaaaa gaggtaccga
     7381 gaaatgagga gaaagaatat cattggtcaa gtttgtgata cgcctaagtc ctatgataat
     7441 gttatgcacg ttggcttgag gaaggtgacc ttcaaatggc aaagaggaaa caaaattgga
     7501 gaaggccagt atgggaaggt gtacacctgc atcagcgtcg acaccgggga gctgatggcc
     7561 atgaaagaga ttcgatttca acctaatgac cataagacta tcaaggaaac tgcagacgaa
     7621 ttgaaaatat tcgaaggcat caaacacccc aatctggttc ggtattttgg tgtggagctc
     7681 catagagaag aaatgtacat cttcatggag tactgcgatg aggggacttt agaagaggtg
     7741 tcaaggctgg gacttcagga acatgtgatt aggctgtatt caaagcagat caccattgcg
     7801 atcaacgtcc tccatgagca tggcatagtc caccgtgaca ttaaaggtgc caatatcttc
     7861 cttacctcat ctggattaat caaactggga gattttggat gttcagtaaa gctcaaaaac
     7921 aatgcccaga ccatgcctgg tgaagtgaac agcaccctgg ggacagcagc atacatggca
     7981 cctgaagtca tcactcgtgc caaaggagag ggccatgggc gtgcggccga catctggagt
     8041 ctggggtgtg ttgtcataga gatggtgact ggcaagaggc cttggcatga gtatgagcac
     8101 aactttcaaa ttatgtataa agtggggatg ggacataagc caccaatccc tgaaagatta
     8161 agccctgaag gaaaggactt cctttctcac tgccttgaga gtgacccaaa gatgagatgg
     8221 accgccagcc agctcctcga ccattcgttt gtcaaggttt gcacagatga agaatgagac
     8281 ccagctttct tgtacaaagt ggtgatatcc aattaaccct cactaaagga ttgtgtttaa
     8341 gaaatatgat gagccctata gtgagtcgta ttaccaccca acttttctat acaaagtggt
     8401 tgatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc
     8461 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggttagtaat gagatccctc
     8521 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     8581 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     8641 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     8701 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     8761 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     8821 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     8881 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     8941 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     9001 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     9061 cctttgaaaa acacgatgat aatatggcca cacatatgac cgagtacaag cccacggtgc
     9121 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     9181 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     9241 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     9301 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     9361 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     9421 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     9481 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     9541 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     9601 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     9661 gcacctggtg catgacccgc aagcccggtg cctaaatcga tagatcctaa tcaacctctg
     9721 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     9781 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     9841 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     9901 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     9961 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
    10021 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
    10081 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
    10141 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
    10201 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
    10261 cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga
    10321 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
    10381 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
    10441 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
    10501 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
    10561 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
    10621 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg
    10681 ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta
    10741 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
    10801 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
    10861 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
    10921 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
    10981 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
    11041 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
    11101 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
    11161 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
    11221 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
    11281 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
    11341 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
    11401 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
    11461 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
    11521 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
    11581 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
    11641 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
    11701 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
    11761 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
    11821 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
    11881 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    11941 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
    12001 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    12061 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
    12121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    12181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    12241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    12301 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    12361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    12421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    12481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    12541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    12601 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    12661 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
    12721 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg
    12781 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
    12841 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac
    12901 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
    12961 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
    13021 gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat
    13081 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    13141 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
    13201 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
    13261 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
