LOCUS       Exported               11782 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Lentiviral expression of PDGFRA P577T.
ACCESSION   .
VERSION     .
KEYWORDS    pHAGE-PDGFRA-P577T
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11782)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11782
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     108..125
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(307..348)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    401..425
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3699..3723)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(3743..4921)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3752..4690
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(3775..3795)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    4969..5086
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5379..5423)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5608..5841
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6338..6463
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6510..7143
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7335..7354)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7404..7420)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7404..7420)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7417..7439)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7428..7444
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7452..7482)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7497..7518
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7647..7666)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(7758..7775)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7929..8517)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8009..8028)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8688..9548)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9311..9330
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9549..9653)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9721..9739
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             9858..10091
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    10166..10754
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             10272..10283
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     10681..10701
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10768..11487)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(10771..11487)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(10813..10834)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     11162..11181
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     11421..11442
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    11499..269
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    11501..286
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(11661..11680)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 gtcgccaggt ggggggttcc gctgcctgca aagggtcgct acagacgttg tttgtcttca
       61 agaagcttcc agaggaactg cttccttcac gacattcaac agaccttgca ttcctttggc
      121 gagaggggaa agacccctag gaatgctcgt caagaagaca gggccaggtt tccgggccct
      181 cacattgcca aaagacggca atatggtgga aaataacata tagacaaacg cacaccggcc
      241 ttattccaag cggcttcggc cagtaacgtt aggggggggg gagggatctc attactaacc
      301 ggtacgcgta gaatcgagac cgaggagagg gttagggata ggcttacctt cgaaccgcgg
      361 gccctctaga ctcgagcggc cgccactgtg ctggatatca accactttgt acaagaaagc
      421 tgggttctac aggaagctgt cttccaccag gtctgaagag tctatgccga tgtcgtccat
      481 catgtcgatg tcttcaatgg tctcgtcctc tctcttgatg aaggtggaac tgctggaacc
      541 cgtctcaatg gcactctctt cagaggtctg cgagctgtgt ctgttcctct tgcccaggtc
      601 ctcctcctca gggacagggt caatgtcagg cagaggaatg atgtagccac tgtcagcgct
      661 cagtctctgc tcatccagac caccctccca gtccttcagc ttgtcttcct cgtttttgta
      721 ggtgacacca atgtatgcat tgtctgagtc cacacgcatg cgtgccacag caggatggtc
      781 actcttcagg aagtccaggt gaattttttc ataactcttt ttatattgtc caggcagcag
      841 attctccaca atctcactca ggtggtaaaa ggagggtctc ttctccggct cactgttcca
      901 gcatttcacc atgatctcgt agacttcact ggtagcgtgg tcaggcttgg ccatccggta
      961 cccactcttg atcttattgt agaaagtaga atccaccatc atgccggggt aaggggtgcc
     1021 accaagggaa aagatctccc agagcagaat gccataagac cagacatcac tcagtgtggt
     1081 gtagaggttg tcaaagatgc tctcaggagc catccacttc acgggcagaa aggtactgcc
     1141 tttcgacaca tagttcgaat catgcatgat gtctctggcc aggccaaagt cacagatctt
     1201 cacaattttt ccttgtgcca ggaggacgtt gcgagcagcc agatcacggt ggacacaatt
     1261 ttttgaagcc aaaaactcca ttcctcgggc aacttgatag gtgaagctca acaaatccaa
     1321 taaagtaagg ccttctgagt tatcatctga aaggaggttt ttgacttctg agtctaacat
     1381 agatttcttc ttatatgagg ctggacgatc atagagtgat ctctggatgt cggaatattt
     1441 agaaacctct ttcctttcta gcatggggac atactgtgta gtatcagcct gcttcatgtc
     1501 catgtagtca ccattgtttt caaaagataa aataacatag ctccgtgtgc tttcatcagc
     1561 agggttcaat ccaaagatat ccagctcttt ctttggcttc tctgggtggt ggctcaggaa
     1621 gctatcccta ttcttatgca aatagttgac caaatctcca tagaagcaat actctgtgat
     1681 gatgtaaatg gggcctgact tggtgcaggc tcccagcaag tttacaatgt tcaaatgtgg
     1741 ccccaggtga gtcattatct tcagttcaga catgagagct tgtttttcac tggatctggc
     1801 cgtgggtttt agcatcttca ctgcaacttt catgacaggt tgggaccggc ttaatccata
     1861 ggctgttcct tcaaccacct tcccaaacgc tccagacccc aagacccgac caagcactag
     1921 tccatctctt ggaaactccc atcttgagtc ataaggcagc tgcatcgtgt ccacataaat
     1981 atattcatgt ccatccgggc tgattgattc aatgaccctc cagcgaattt catacctcgg
     2041 tttctgtttc caaatgacaa ccaggacaat aagtgagatg atcacaatca ccaacagcac
     2101 caggactgca gcagccaccg tgagttcaga acgcagggtg ggagccacca gcttcagctc
     2161 tcggttctca gctccaagga gattcttagc caggcatcgc acggcgatgg tctcctccac
     2221 tttggcgaaa gtcacacggc cctccacggt actcctgtct cgggagtgga tctccgtgat
     2281 gatgtttgag acattgttgg ccaaaatagt ccaggaagtt tcattattac atttcttaat
     2341 atctttgcat atcatccact caatatcagg aagcggcgtg ccttcagctg tgcacctcac
     2401 cgtctgtccc ccagttgagc catggtgatc atcgaccaag tccagaatgg atgaaggaac
     2461 ttgagttaac agttcaaaag tatagctctt cacagcatct tcattttgag ctacaatagt
     2521 ataatggcca ctgtcttctt ccttagcacg gatcagcttt aatttgcttc gataccttat
     2581 ttcctgaatc ttttccacat cagtggtgat ctcagtgaga ttttcaatca gagtcagatt
     2641 gtttttcagc caggatatcc tgggaggtgg gtaggcccgc acctctacaa caaaatgttt
     2701 gacttcatgc aggttgacag cttccaactg gctgaaggtg ggtttgattt caatgaaacc
     2761 tttctcatgg acagaaatag tgactttctt catttctttg acctccctgg tagcctggcg
     2821 ggcagcacat tcgtaatctc cactgtcttt caccgtggcc tcggggaccg tcaaagtgta
     2881 caccaatttg atggatggga ctttgatttc ttccagcatt gtgatgcctt tgcctttcac
     2941 ttctccaggg taagtccatt gaaggtcaac cacctcattg ttaaaaacag cacaggtgac
     3001 cacaatcgtt tcccctgact tatacacggt tttaagagct tccatttcta gatccagctc
     3061 tgatgttgct tttaaagcat aaacattaaa tgggatggtc tggaacttct ttcctttgac
     3121 ggtggcctca cagatatagg gccctacagt gaaggtccca ttaaagccct gtctgctgtc
     3181 gtaggaggca ggtaccaccc cctcactgtt gtgtaaggtt acaggagtct cgggatcagt
     3241 tgtgcgacaa ggtataatgg cagaatcatc atcctccacg atgactaaat aatccgtcat
     3301 tcctagaggt acaaaggcta catctgggtc tggcacatag atgtaaatgt gcctgccttc
     3361 aagctcattc tcttctgtct gagtgtggtt gtaatagcaa gtgtacaacc ctgtgtgggc
     3421 cgccgaggca ctgctcactt ccaagaccgt cacaaaaagg ccgctgttgt tttcttcatt
     3481 tctgatttcc acatcggagc tctcttcttc agacatgggg tactgccagc tcacttcact
     3541 ctccccaaag catctcagag aaaaggatga attcagctgc acaacctttt cattttcatt
     3601 tggaaggata gagggtaatg aaagctggca gaggattagg ctcagccctg tgagaagaca
     3661 gcctaagacc aggaacgccg gatgggaagt ccccatggtg cctgcttttt tgtacaaact
     3721 tgttgatatc tgcagggccg cttcacgaca cctgaaatgg aagaaaaaaa ctttgaacca
     3781 ctgtctgagg cttgagaatg aaccaagatc caaactcaaa aagggcaaat tccaaggaga
     3841 attacatcaa gtgccaagct ggcctaactt cagtctccac ccactcagtg tggggaaact
     3901 ccatcgcata aaacccctcc ccccaaccta aagacgacgt actccaaaag ctcgagaact
     3961 aatcgaggtg cctggacggc gcccggtact ccgtggagtc acatgaagcg acggctgagg
     4021 acggaaaggc ccttttcctt tgtgtgggtg actcacccgc ccgctctccc gagcgccgcg
     4081 tcctccattt tgagctccct gcagcagggc cgggaagcgg ccatctttcc gctcacgcaa
     4141 ctggtgccga ccgggccagc cttgccgccc agggcggggc gatacacggc ggcgcgaggc
     4201 caggcaccag agcaggccgg ccagcttgag actacccccg tccgattctc ggtggccgcg
     4261 ctcgcaggcc ccgcctcgcc gaacatgtgc gctgggacgc acgggccccg tcgccgcccg
     4321 cggccccaaa aaccgaaata ccagtgtgca gatcttggcc cgcatttaca agactatctt
     4381 gccagaaaaa aagcgtcgca gcaggtcatc aaaaatttta aatggctaga gacttatcga
     4441 aagcagcgag acaggcgcga aggtgccacc agattcgcac gcggcggccc cagcgcccag
     4501 gccaggcctc aactcaagca cgaggcgaag gggctcctta agcgcaaggc ctcgaactct
     4561 cccacccact tccaacccga agctcgggat caagaatcac gtactgcagc caggtggaag
     4621 taattcaagg cacgcaaggg ccataacccg taaagaggcc aggcccgcgg gaaccacaca
     4681 cggcacttac ctgtgttctg gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta
     4741 ctgcacttat atacggttct cccccaccct cgggaaaaag gcggagccag tacacgacat
     4801 cactttccca gtttaccccg cgccaccttc tctaggcacc ggttcaattg ccgacccctc
     4861 cccccaactt ctcggggact gtgggcgatg tgcgctctgc ccactgacgg gcaccggagc
     4921 ctcacgacta gtccaaactg gatctctgct gtccctgtaa taaacccgaa aattttgaat
     4981 ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat
     5041 tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac
     5101 tgccatttgt gaattcggcg ataccgtcga gatccgttca ctaatcgaat ggatctgtct
     5161 ctgtctctct ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt
     5221 gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa
     5281 agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat gaataatttt
     5341 atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat
     5401 tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt
     5461 attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa
     5521 atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg
     5581 agttttccag agcaacccca aatccccagg agctgttgat cctttaggta tctttccaca
     5641 gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa cagatgctgt
     5701 tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga caataattgt
     5761 ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc tgctcccaag
     5821 aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac cactcttctc
     5881 tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata tttatataat
     5941 tcacttctcc aattgtccct catatctcct cctccaggtc tgaagatcag cggccggccg
     6001 cttgctgtgc ggtggtctta cttttgtttt gctcttcctc tatcttgtct aaagcttcct
     6061 tggtgtcttt tatctctatc ctttgatgca cacaatagag ggttgctact gtattatata
     6121 atgatctaag ttcttctgat cctgtctgaa gggatggttg tagctgtccc agtatttgtc
     6181 tacagccttc tgatgtttct aacaggccag gattaactgc gaatcgttct agctccctgc
     6241 ttgcccatac tatatgtttt aatttatatt ttttctttcc ccctggcctt aaccgaattt
     6301 tttcccatcg cgatctaatt ctcccccgct taatactgac gctctcgcac ccatctctct
     6361 ccttctagcc tccgctagtc aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc
     6421 ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga gagctcctct ggtttccctt
     6481 tcgctttcaa gtccctgttc gggcgccact gctagagatt ttccacactg actaaaaggg
     6541 tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac
     6601 tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     6661 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagct gcttatatgc
     6721 aggatctgag ggctcgccac tccccagtcc cgcccaggcc acgcctccct ggaaagtccc
     6781 cagcggaaag tcccttgtag caagctcgat atcagcagtt cttgaagtac tccggatgca
     6841 gctctcgggc cacgtgatga aatgctaggc ggctgtcaaa cctccactct aacacttctc
     6901 tctccgggtc atccatccca tgcaggctca cagggtgtaa caagctggtg ttctctcctt
     6961 tattggcctc ttctacctta tctggctcaa ctggtactag cttgtagcac catccaaagg
     7021 tcagtggata tctgacccct ggccctggtg tgtagttctg ctaatcaggg aagtagcctt
     7081 gtgtgtggta gatccacaga tcaaggatat cttgtcttct ttgggagtga attagccctt
     7141 ccaactacta agtttgtagt acatatttaa caaatacaat ttctttaaaa tgaaaataat
     7201 tcagaggaat cacaggttta gagtaaatga aaccacaggt aattggcagt ggtaataggg
     7261 tatggggtgg gaagtttggg atgattttgg ttagcttgag ttatccagtt gatccagaca
     7321 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     7381 ttatttgtga aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7441 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7501 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7561 tcgtgccacg gggagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
     7621 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
     7681 gccatgagct tgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7741 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7801 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7861 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7921 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7981 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8041 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8101 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8161 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8221 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8281 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8341 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     8401 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8461 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8521 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8581 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8641 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8701 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8761 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8821 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8881 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8941 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9001 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9061 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9121 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9181 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9241 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9301 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9361 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9421 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9481 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9541 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9601 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9661 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     9721 aataggcgta tcacgaggcc ctttcgtctt caagaatgat ctagcccttt ccttaattaa
     9781 cccgggcctc tcactctctg atattcattt ctttgcaagt tataaatact gaataataag
     9841 atgacatgaa ctactactgc tagagatttt ccacactgac taaaagggtc tgagggatct
     9901 ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct
     9961 ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg
    10021 gtctaaccag agagacccag tacaagcaaa aagcagatct tgtcttcgtt gggagtgaat
    10081 tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta
    10141 agtcattggt cttaaaggat ctcaggcggg gaggcggccc aaagggagat ccgactcgtc
    10201 tgagggcgaa ggcgaagacg cggaagaggc cgcagagccg gcagcaggcc gcgggaagga
    10261 aggtccgctg gattgagggc cgaagggacg tagcagaagg acgtcccgcg cagaatccag
    10321 gtggcaacac aggcgagcag ccaaggaaag gacgatgatt tccccgacaa caccacggaa
    10381 ttgtcagtgc ccaacagccg agcccctgtc cagcagcggg caaggcaggc ggcgatgagt
    10441 tccgccgtgg caatagggag ggggaaagcg aaagtcccgg aaaggagctg acaggtggtg
    10501 gcaatgcccc aaccagtggg ggttgcgtca gcaaacacag tgcacaccac gccacgttgc
    10561 ctgacaacgg gccacaactc ctcataaaga gacagcaacc aggatttata caaggaggag
    10621 aaaatgaaag ccatacggga agcaatagca tgatacaaag gcattaaagc agcgtatcca
    10681 catagcgtaa aaggagcaac atagttaaga ataccagtca atctttcaca aattttgtaa
    10741 tccagaggtt gattaggatc tatcgattta cttgtacagc tcgtccatgc cgagagtgat
    10801 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
    10861 gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc
    10921 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg
    10981 gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt
    11041 gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc
    11101 gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg
    11161 ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg
    11221 catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg
    11281 cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt
    11341 gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac
    11401 gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt
    11461 gaacagctcc tcgcccttgc tcaccatatg tgtggccata ttatcatcgt gtttttcaaa
    11521 ggaaaaccac gtccccgtgg ttcggggggc ctagacgttt ttttaacctc gactaaacac
    11581 atgtaaagca tgtgcaccga ggccccagat cagatcccat acaatggggt accttctggg
    11641 catccttcag ccccttgttg aatacgcttg aggagagcca tttgactctt tccacaacta
    11701 tccaactcac aacgtggcac tggggttgtg ccgcctttgc aggtgtatct tatacacgtg
    11761 gcttttggcc gcagaggcac ct
//
