LOCUS Exported 11782 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of PDGFRA Y288C. ACCESSION . VERSION . KEYWORDS pHAGE-PDGFRA-Y288C SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11782) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 11782) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11782 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(6719..6743) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 6796..6837 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 6858..7425 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 6875..7427 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(7019..7036) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 7246..7265 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 7439..8158 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 7439..8155 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(7484..7505) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(7745..7764) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 8092..8113 /label=EGFP-C /note="EGFP, forward primer" misc_feature 8172..8760 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8225..8245) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8643..8654) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 8835..9068 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(9187..9205) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 9273..9377 /gene="bla" /label=AmpR promoter CDS 9378..10238 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9596..9615) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10409..10997 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10898..10917 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11151..11168 /label=L4440 /note="L4440 vector, forward primer" primer_bind 11260..11279 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 11408..11429 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 11444..11474 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11482..11498 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 11487..11509 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 11506..11522 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 11506..11522 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 11572..11591 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcaccatg gggacttccc atccggcgtt cctggtctta 3481 ggctgtcttc tcacagggct gagcctaatc ctctgccagc tttcattacc ctctatcctt 3541 ccaaatgaaa atgaaaaggt tgtgcagctg aattcatcct tttctctgag atgctttggg 3601 gagagtgaag tgagctggca gtaccccatg tctgaagaag agagctccga tgtggaaatc 3661 agaaatgaag aaaacaacag cggccttttt gtgacggtct tggaagtgag cagtgcctcg 3721 gcggcccaca cagggttgta cacttgctat tacaaccaca ctcagacaga agagaatgag 3781 cttgaaggca ggcacattta catctatgtg ccagacccag atgtagcctt tgtacctcta 3841 ggaatgacgg attatttagt catcgtggag gatgatgatt ctgccattat accttgtcgc 3901 acaactgatc ccgagactcc tgtaacctta cacaacagtg agggggtggt acctgcctcc 3961 tacgacagca gacagggctt taatgggacc ttcactgtag ggccctatat ctgtgaggcc 4021 accgtcaaag gaaagaagtt ccagaccatc ccatttaatg tttatgcttt aaaagcaaca 4081 tcagagctgg atctagaaat ggaagctctt aaaaccgtgt ataagtcagg ggaaacgatt 4141 gtggtcacct gtgctgtttt taacaatgag gtggttgacc ttcaatggac ttaccctgga 4201 gaagtgaaag gcaaaggcat cacaatgctg gaagaaatca aagtcccatc catcaaattg 4261 gtgtacactt tgacggtccc cgaggccacg gtgaaagaca gtggagattg cgaatgtgct 4321 gcccgccagg ctaccaggga ggtcaaagaa atgaagaaag tcactatttc tgtccatgag 4381 aaaggtttca ttgaaatcaa acccaccttc agccagttgg aagctgtcaa cctgcatgaa 4441 gtcaaacatt ttgttgtaga ggtgcgggcc tacccacctc ccaggatatc ctggctgaaa 4501 aacaatctga ctctgattga aaatctcact gagatcacca ctgatgtgga aaagattcag 4561 gaaataaggt atcgaagcaa attaaagctg atccgtgcta aggaagaaga cagtggccat 4621 tatactattg tagctcaaaa tgaagatgct gtgaagagct atacttttga actgttaact 4681 caagttcctt catccattct ggacttggtc gatgatcacc atggctcaac tgggggacag 4741 acggtgaggt gcacagctga aggcacgccg cttcctgata ttgagtggat gatatgcaaa 4801 gatattaaga aatgtaataa tgaaacttcc tggactattt tggccaacaa tgtctcaaac 4861 atcatcacgg agatccactc ccgagacagg agtaccgtgg agggccgtgt gactttcgcc 4921 aaagtggagg agaccatcgc cgtgcgatgc ctggctaaga atctccttgg agctgagaac 4981 cgagagctga agctggtggc tcccaccctg cgttctgaac tcacggtggc tgctgcagtc 5041 ctggtgctgt tggtgattgt gatcatctca cttattgtcc tggttgtcat ttggaaacag 5101 aaaccgaggt atgaaattcg ctggagggtc attgaatcaa tcagcccgga tggacatgaa 5161 tatatttatg tggacccgat gcagctgcct tatgactcaa gatgggagtt tccaagagat 5221 ggactagtgc ttggtcgggt cttggggtct ggagcgtttg ggaaggtggt tgaaggaaca 5281 gcctatggat taagccggtc ccaacctgtc atgaaagttg cagtgaagat gctaaaaccc 5341 acggccagat ccagtgaaaa acaagctctc atgtctgaac tgaagataat gactcacctg 5401 gggccacatt tgaacattgt aaacttgctg ggagcctgca ccaagtcagg ccccatttac 5461 atcatcacag agtattgctt ctatggagat ttggtcaact atttgcataa gaatagggat 5521 agcttcctga gccaccaccc agagaagcca aagaaagagc tggatatctt tggattgaac 5581 cctgctgatg aaagcacacg gagctatgtt attttatctt ttgaaaacaa tggtgactac 5641 atggacatga agcaggctga tactacacag tatgtcccca tgctagaaag gaaagaggtt 5701 tctaaatatt ccgacatcca gagatcactc tatgatcgtc cagcctcata taagaagaaa 5761 tctatgttag actcagaagt caaaaacctc ctttcagatg ataactcaga aggccttact 5821 ttattggatt tgttgagctt cacctatcaa gttgcccgag gaatggagtt tttggcttca 5881 aaaaattgtg tccaccgtga tctggctgct cgcaacgtcc tcctggcaca aggaaaaatt 5941 gtgaagatct gtgactttgg cctggccaga gacatcatgc atgattcgaa ctatgtgtcg 6001 aaaggcagta cctttctgcc cgtgaagtgg atggctcctg agagcatctt tgacaacctc 6061 tacaccacac tgagtgatgt ctggtcttat ggcattctgc tctgggagat cttttccctt 6121 ggtggcaccc cttaccccgg catgatggtg gattctactt tctacaataa gatcaagagt 6181 gggtaccgga tggccaagcc tgaccacgct accagtgaag tctacgagat catggtgaaa 6241 tgctggaaca gtgagccgga gaagagaccc tccttttacc acctgagtga gattgtggag 6301 aatctgctgc ctggacaata taaaaagagt tatgaaaaaa ttcacctgga cttcctgaag 6361 agtgaccatc ctgctgtggc acgcatgcgt gtggactcag acaatgcata cattggtgtc 6421 acctacaaaa acgaggaaga caagctgaag gactgggagg gtggtctgga tgagcagaga 6481 ctgagcgctg acagtggcta catcattcct ctgcctgaca ttgaccctgt ccctgaggag 6541 gaggacctgg gcaagaggaa cagacacagc tcgcagacct ctgaagagag tgccattgag 6601 acgggttcca gcagttccac cttcatcaag agagaggacg agaccattga agacatcgac 6661 atgatggacg acatcggcat agactcttca gacctggtgg aagacagctt cctgtagaac 6721 ccagctttct tgtacaaagt ggttgatatc cagcacagtg gcggccgctc gagtctagag 6781 ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt 6841 accggttagt aatgagatcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa 6901 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat 6961 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct 7021 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct 7081 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc 7141 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa 7201 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc 7261 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg 7321 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc 7381 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg ccacacatat 7441 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg 7501 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg 7561 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct 7621 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca 7681 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt 7741 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt 7801 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa 7861 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg 7921 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga 7981 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta 8041 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct 8101 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaat 8161 cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta 8221 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta 8281 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt 8341 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 8401 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 8461 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag 8521 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc 8581 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc 8641 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc 8701 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc 8761 ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 8821 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc 8881 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 8941 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 9001 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 9061 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 9121 aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat tcttgaagac 9181 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt 9241 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct 9301 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat 9361 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg 9421 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg 9481 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc 9541 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat 9601 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact 9661 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca 9721 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact 9781 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg 9841 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg 9901 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg 9961 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg 10021 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag 10081 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc 10141 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga 10201 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat 10261 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 10321 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 10381 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 10441 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 10501 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc 10561 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 10621 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 10681 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 10741 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 10801 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 10861 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 10921 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 10981 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 11041 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 11101 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag 11161 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga 11221 ttcattaatg cagcaagctc atggctgact aatttttttt atttatgcag aggccgaggc 11281 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 11341 ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 11401 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 11461 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 11521 acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 11581 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa 11641 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt 11701 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa 11761 tatgtactac aaacttagta gt //