LOCUS Exported 11687 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of PIK3CA E109_I112delinsD. ACCESSION . VERSION . KEYWORDS pHAGE-PIK3CA-E109_I112delinsD SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11687) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 11687) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11687 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(6647..6671) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 6679..6697 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(6722..6741) /label=T7 /note="T7 promoter, forward primer" promoter complement(6723..6741) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(6747..6767) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 6821..6862 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 6883..7450 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 6900..7452 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(7044..7061) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 7271..7290 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 7464..8063 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(7464..7483) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 7960..7980 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 8077..8665 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8130..8150) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8548..8559) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 8740..8973 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(9092..9110) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 9178..9282 /gene="bla" /label=AmpR promoter CDS 9283..10143 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9501..9520) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10314..10902 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10803..10822 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11056..11073 /label=L4440 /note="L4440 vector, forward primer" primer_bind 11165..11184 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 11313..11334 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 11349..11379 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11387..11403 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 11392..11414 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 11411..11427 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 11411..11427 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 11477..11496 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcaccatg cctccaagac catcatcagg tgaactgtgg 3481 ggcatccact tgatgccccc aagaatccta gtagaatgtt tactaccaaa tggaatgata 3541 gtgactttag aatgcctccg tgaggctaca ttaataacca taaagcatga actatttaaa 3601 gaagcaagaa aataccccct ccatcaactt cttcaagatg aatcttctta cattttcgta 3661 agtgttactc aagaagcaga aagggaagaa ttttttgatg aaacaagacg actttgtgac 3721 cttcggcttt ttcaaccctt tttaaaagta attgaaccag taggcaaccg tgacctcaat 3781 cgagaaattg gttttgctat cggcatgcca gtgtgtgaat ttgatatggt taaagatcca 3841 gaagtacagg acttccgaag aaatattctg aacgtttgta aagaagctgt ggatcttagg 3901 gacctcaatt cacctcatag tagagcaatg tatgtctatc ctccaaatgt agaatcttca 3961 ccagaattgc caaagcacat atataataaa ttagataaag ggcaaataat agtggtgatc 4021 tgggtaatag tttctccaaa taatgacaag cagaagtata ctctgaaaat caaccatgac 4081 tgtgtaccag aacaagtaat tgctgaagca atcaggaaaa aaactcgaag tatgttgcta 4141 tcctctgaac aactaaaact ctgtgtttta gaatatcagg gcaagtatat tttaaaagtg 4201 tgtggatgtg atgaatactt cctagaaaaa tatcctctga gtcagtataa gtatataaga 4261 agctgtataa tgcttgggag gatgcccaat ttgatgttga tggctaaaga aagcctttat 4321 tctcaactgc caatggactg ttttacaatg ccatcttatt ccagacgcat ttccacagct 4381 acaccatata tgaatggaga aacatctaca aaatcccttt gggttataaa tagtgcactc 4441 agaataaaaa ttctttgtgc aacctacgtg aatgtaaata ttcgagacat tgataagatc 4501 tatgttcgaa caggtatcta ccatggagga gaacccttat gtgacaatgt gaacactcaa 4561 agagtacctt gttccaatcc caggtggaat gaatggctga attatgatat atacattcct 4621 gatcttcctc gtgctgctcg actttgcctt tccatttgct ctgttaaagg ccgaaagggt 4681 gctaaagagg aacactgtcc attggcatgg ggaaatataa acttgtttga ttacacagac 4741 actctagtat ctggaaaaat ggctttgaat ctttggccag tacctcatgg attagaagat 4801 ttgctgaacc ctattggtgt tactggatca aatccaaata aagaaactcc atgcttagag 4861 ttggagtttg actggttcag cagtgtggta aagttcccag atatgtcagt gattgaagag 4921 catgccaatt ggtctgtatc ccgagaagca ggatttagct attcccacgc aggactgagt 4981 aacagactag ctagagacaa tgaattaagg gaaaatgaca aagaacagct caaagcaatt 5041 tctacacgag atcctctctc tgaaatcact gagcaggaga aagattttct atggagtcac 5101 agacactatt gtgtaactat ccccgaaatt ctacccaaat tgcttctgtc tgttaaatgg 5161 aattctagag atgaagtagc ccagatgtat tgcttggtaa aagattggcc tccaatcaaa 5221 cctgaacagg ctatggaact tctggactgt aattacccag atcctatggt tcgaggtttt 5281 gctgttcggt gcttggaaaa atatttaaca gatgacaaac tttctcagta tttaattcag 5341 ctagtacagg tcctaaaata tgaacaatat ttggataact tgcttgtgag atttttactg 5401 aagaaagcat tgactaatca aaggattggg cactttttct tttggcattt aaaatctgag 5461 atgcacaata aaacagttag ccagaggttt ggcctgcttt tggagtccta ttgtcgtgca 5521 tgtgggatgt atttgaagca cctgaatagg caagtcgagg caatggaaaa gctcattaac 5581 ttaactgaca ttctcaaaca ggagaagaag gatgaaacac aaaaggtaca gatgaagttt 5641 ttagttgagc aaatgaggcg accagatttc atggatgctc tacagggctt tctgtctcct 5701 ctaaaccctg ctcatcaact aggaaacctc aggcttgaag agtgtcgaat tatgtcctct 5761 gcaaaaaggc cactgtggtt gaattgggag aacccagaca tcatgtcaga gttactgttt 5821 cagaacaatg agatcatctt taaaaatggg gatgatttac ggcaagatat gctaacactt 5881 caaattattc gtattatgga aaatatctgg caaaatcaag gtcttgatct tcgaatgtta 5941 ccttatggtt gtctgtcaat cggtgactgt gtgggactta ttgaggtggt gcgaaattct 6001 cacactatta tgcaaattca gtgcaaaggc ggcttgaaag gtgcactgca gttcaacagc 6061 cacacactac atcagtggct caaagacaag aacaaaggag aaatatatga tgcagccatt 6121 gacctgttta cacgttcatg tgctggatac tgtgtagcta ccttcatttt gggaattgga 6181 gatcgtcaca atagtaacat catggtgaaa gacgatggac aactgtttca tatagatttt 6241 ggacactttt tggatcacaa gaagaaaaaa tttggttata aacgagaacg tgtgccattt 6301 gttttgacac aggatttctt aatagtgatt agtaaaggag cccaagaatg cacaaagaca 6361 agagaatttg agaggtttca ggagatgtgt tacaaggctt atctagctat tcgacagcat 6421 gccaatctct tcataaatct tttctcaatg atgcttggct ctggaatgcc agaactacaa 6481 tcttttgatg acattgcata cattcgaaag accctagcct tagataaaac tgagcaagag 6541 gctttggagt atttcatgaa acaaatgaat gatgcacatc atggtggctg gacaacaaaa 6601 atggattgga tcttccacac aattaaacag catgcattga actagaaccc agctttcttg 6661 tacaaagtgg tgatatccaa ttaaccctca ctaaaggagt aagtgttaga tagtattgaa 6721 tccctatagt gagtcgtatt accacccaac ttttctatac aaagtggttg atatccagca 6781 cagtggcggc cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc 6841 tctcctcggt ctcgattcta cgcgtaccgg ttagtaatga gatccctccc ccccccctaa 6901 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc 6961 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac 7021 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt 7081 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg 7141 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata 7201 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga 7261 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt 7321 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc 7381 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac 7441 acgatgataa tatggccaca catatgaccg agtacaagcc cacggtgcgc ctcgccaccc 7501 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca 7561 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct 7621 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg 7681 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc 7741 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc 7801 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg 7861 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc 7921 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc 7981 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca 8041 tgacccgcaa gcccggtgcc taaatcgata gatcctaatc aacctctgga ttacaaaatt 8101 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 8161 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 8221 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 8281 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 8341 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 8401 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 8461 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg 8521 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 8581 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg 8641 atctcccttt gggccgcctc cccgcctgag atcctttaag accaatgact tacaaggcag 8701 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc 8761 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag 8821 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt 8881 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca 8941 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc 9001 agtatttata acttgcaaag aaatgaatat cagagagtga gaggcccggg ttaattaagg 9061 aaagggctag atcattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt 9121 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc 9181 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 9241 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 9301 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 9361 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 9421 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 9481 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa 9541 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 9601 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 9661 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 9721 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 9781 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 9841 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 9901 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 9961 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 10021 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 10081 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 10141 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 10201 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 10261 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 10321 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 10381 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 10441 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac 10501 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 10561 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 10621 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 10681 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 10741 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 10801 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 10861 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 10921 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 10981 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 11041 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa 11101 ccgcctctcc ccgcgcgttg gccgattcat taatgcagca agctcatggc tgactaattt 11161 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 11221 gaggcttttt tggaggccta ggcttttgca aaaagctccc cgtggcacga caggtttccc 11281 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca 11341 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 11401 caatttcaca caggaaacag ctatgacatg attacgaatt tcacaaataa agcatttttt 11461 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc 11521 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc 11581 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca 11641 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagt //