LOCUS Exported 10300 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of ESR1 E247K. ACCESSION . VERSION . KEYWORDS pHAGE-ESR1-E247K SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10300) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 10300) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10300 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 4342..5235 /codon_start=1 /gene="ESR1" /product="estrogen nuclear receptor alpha ligand-binding domain" /label=ER-LBD /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL QKYYITGEAEGFPATV" protein_bind complement(5237..5261) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 5314..5355 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 5376..5943 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 5393..5945 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5537..5554) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5764..5783 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5957..6676 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 5957..6673 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(6002..6023) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6263..6282) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6610..6631 /label=EGFP-C /note="EGFP, forward primer" misc_feature 6690..7278 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6743..6763) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7161..7172) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7353..7586 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7705..7723) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7791..7895 /gene="bla" /label=AmpR promoter CDS 7896..8756 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8114..8133) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8927..9515 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9416..9435 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9669..9686 /label=L4440 /note="L4440 vector, forward primer" primer_bind 9778..9797 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 9926..9947 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9962..9992 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10000..10016 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 10005..10027 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 10024..10040 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 10024..10040 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 10090..10109 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcaccatg accatgaccc tccacaccaa agcatccggg 3481 atggccctac tgcatcagat ccaagggaac gagctggagc ccctgaaccg tccgcagctc 3541 aagatccccc tggagcggcc cctgggcgag gtgtacctgg acagcagcaa gcccgccgtg 3601 tacaactacc ccgagggcgc cgcctacgag ttcaacgccg cggccgccgc caacgcgcag 3661 gtctacggtc agaccggcct cccctacggc cccgggtctg aggctgcggc gttcggctcc 3721 aacggcctgg ggggtttccc cccactcaac agcgtgtctc cgagcccgct gatgctactg 3781 cacccgccgc cgcagctgtc gcctttcctg cagccccacg gccagcaggt gccctactac 3841 ctggagaacg agcccagcgg ctacacggtg cgcgaggccg gcccgccggc attctacagg 3901 ccaaattcag ataatcgacg ccagggtggc agagaaagat tggccagtac caatgacaag 3961 ggaagtatgg ctatggaatc tgccaaggag actcgctact gtgcagtgtg caatgactat 4021 gcttcaggct accattatgg agtctggtcc tgtgagggct gcaaggcctt cttcaagaga 4081 agtattcaag gacataacga ctatatgtgt ccagccacca accagtgcac cattgataaa 4141 aacaggagga agagctgcca ggcctgccgg ctccgcaaat gctacaaagt gggaatgatg 4201 aaaggtggga tacgaaaaga ccgaagagga gggagaatgt tgaaacacaa gcgccagaga 4261 gatgatgggg agggcagggg tgaagtgggg tctgctggag acatgagagc tgccaacctt 4321 tggccaagcc cgctcatgat caaacgctct aagaagaaca gcctggcctt gtccctgacg 4381 gccgaccaga tggtcagtgc cttgttggat gctgagcccc cgatactcta ttccgagtat 4441 gatcctacca gacccttcag tgaagcttcg atgatgggct tactgaccaa cctggcagac 4501 agggagctgg ttcacatgat caactgggcg aagagggtgc caggctttgt ggatttgacc 4561 ctccatgatc aggtccacct tctagaatgt gcctggctag agatcctgat gattggtctc 4621 gtctggcgct ccatggagca cccagggaag ctactgtttg ctcctaactt gctcttggac 4681 aggaaccagg gaaaatgtgt agagggcatg gtggagatct tcgacatgct gctggctaca 4741 tcatctcggt tccgcatgat gaatctgcag ggagaggagt ttgtgtgcct caaatctatt 4801 attttgctta attctggagt gtacacattt ctgtccagca ccctgaagtc tctggaagag 4861 aaggaccata tccaccgagt cctggacaag atcacagaca ctttgatcca cctgatggcc 4921 aaggcaggcc tgaccctgca gcagcagcac cagcggctgg cccagctcct cctcatcctc 4981 tcccacatca ggcacatgag taacaaaggc atggagcatc tgtacagcat gaagtgcaag 5041 aacgtggtgc ccctctatga cctgctgctg gagatgctgg acgcccaccg cctacatgcg 5101 cccactagcc gtggaggggc atccgtggag gagacggacc aaagccactt ggccactgcg 5161 ggctctactt catcgcattc cttgcaaaag tattacatca cgggggaggc agagggtttc 5221 cctgccacag tctagaaccc agctttcttg tacaaagtgg ttgatatcca gcacagtggc 5281 ggccgctcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc 5341 ggtctcgatt ctacgcgtac cggttagtaa tgagatccct cccccccccc taacgttact 5401 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata 5461 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt 5521 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa 5581 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag 5641 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca 5701 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc 5761 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat 5821 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta 5881 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga 5941 taatatggcc acacatatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat 6001 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga 6061 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc 6121 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta 6181 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca 6241 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt 6301 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg 6361 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc 6421 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg 6481 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct 6541 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa 6601 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga 6661 cgagctgtac aagtaaatcg atagatccta atcaacctct ggattacaaa atttgtgaaa 6721 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 6781 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 6841 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 6901 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 6961 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 7021 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 7081 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 7141 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 7201 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc 7261 tttgggccgc ctccccgcct gagatccttt aagaccaatg acttacaagg cagctgtaga 7321 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag 7381 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 7441 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 7501 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 7561 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 7621 ataacttgca aagaaatgaa tatcagagag tgagaggccc gggttaatta aggaaagggc 7681 tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca 7741 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 7801 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 7861 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 7921 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 7981 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 8041 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 8101 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac 8161 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 8221 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 8281 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 8341 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 8401 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 8461 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 8521 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 8581 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 8641 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 8701 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 8761 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 8821 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 8881 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 8941 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 9001 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 9061 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 9121 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 9181 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 9241 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 9301 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 9361 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 9421 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 9481 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 9541 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 9601 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 9661 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc 9721 tccccgcgcg ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat 9781 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 9841 ttttggaggc ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga 9901 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg 9961 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc 10021 acacaggaaa cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc 10081 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga 10141 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca 10201 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa 10261 gaaattgtat ttgttaaata tgtactacaa acttagtagt //