LOCUS Exported 10303 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of ESR1 E380Q. ACCESSION . VERSION . KEYWORDS pHAGE-ESR1-E380Q SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10303) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 10303) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10303 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 4345..5238 /codon_start=1 /gene="ESR1" /product="estrogen nuclear receptor alpha ligand-binding domain" /label=ER-LBD /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLQCAWLEILMIGLVWRSMEHPGKL LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL QKYYITGEAEGFPATV" protein_bind complement(5240..5264) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 5317..5358 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 5379..5946 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 5396..5948 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5540..5557) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5767..5786 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5960..6679 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 5960..6676 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(6005..6026) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6266..6285) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6613..6634 /label=EGFP-C /note="EGFP, forward primer" misc_feature 6693..7281 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6746..6766) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7164..7175) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7356..7589 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7708..7726) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7794..7898 /gene="bla" /label=AmpR promoter CDS 7899..8759 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8117..8136) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8930..9518 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9419..9438 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9672..9689 /label=L4440 /note="L4440 vector, forward primer" primer_bind 9781..9800 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 9929..9950 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9965..9995 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10003..10019 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 10008..10030 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 10027..10043 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 10027..10043 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 10093..10112 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcttcacc atgaccatga ccctccacac caaagcatct 3481 gggatggccc tactgcatca gatccaaggg aacgagctgg agcccctgaa ccgtccgcag 3541 ctcaagatcc ccctggagcg gcccctgggc gaggtgtacc tggacagcag caagcccgcc 3601 gtgtacaact accccgaggg cgccgcctac gagttcaacg ccgcggccgc cgccaacgcg 3661 caggtctacg gtcagaccgg cctcccctac ggccccgggt ctgaggctgc ggcgttcggc 3721 tccaacggcc tggggggttt ccccccactc aacagcgtgt ctccgagccc gctgatgcta 3781 ctgcacccgc cgccgcagct gtcgcctttc ctgcagcccc acggccagca ggtgccctac 3841 tacctggaga acgagcccag cggctacacg gtgcgcgagg ccggcccgcc ggcattctac 3901 aggccaaatt cagataatcg acgccagggt ggcagagaaa gattggccag taccaatgac 3961 aagggaagta tggctatgga atctgccaag gagactcgct actgtgcagt gtgcaatgac 4021 tatgcttcag gctaccatta tggagtctgg tcctgtgagg gctgcaaggc cttcttcaag 4081 agaagtattc aaggacataa cgactatatg tgtccagcca ccaaccagtg caccattgat 4141 aaaaacagga ggaagagctg ccaggcctgc cggctccgca aatgctacga agtgggaatg 4201 atgaaaggtg ggatacgaaa agaccgaaga ggagggagaa tgttgaaaca caagcgccag 4261 agagatgatg gggagggcag gggtgaagtg gggtctgctg gagacatgag agctgccaac 4321 ctttggccaa gcccgctcat gatcaaacgc tctaagaaga acagcctggc cttgtccctg 4381 acggccgacc agatggtcag tgccttgttg gatgctgagc cccccatact ctattccgag 4441 tatgatccta ccagaccctt cagtgaagct tcgatgatgg gcttactgac caacctggca 4501 gacagggagc tggttcacat gatcaactgg gcgaagaggg tgccaggctt tgtggatttg 4561 accctccatg atcaggtcca ccttctacaa tgtgcctggc tagagatcct gatgattggt 4621 ctcgtctggc gctccatgga gcacccaggg aagctactgt ttgctcctaa cttgctcttg 4681 gacaggaacc agggaaaatg tgtagagggc atggtggaga tcttcgacat gctgctggct 4741 acatcatctc ggttccgcat gatgaatctg cagggagagg agtttgtgtg cctcaaatct 4801 attattttgc ttaattctgg agtgtacaca tttctgtcca gcaccctgaa gtctctggaa 4861 gagaaggacc atatccaccg agtcctggac aagatcacag acactttgat ccacctgatg 4921 gccaaggcag gcctgaccct gcagcagcag caccagcggc tggcccagct cctcctcatc 4981 ctctcccaca tcaggcacat gagtaacaaa ggcatggagc atctgtacag catgaagtgc 5041 aagaacgtgg tgcccctcta tgacctgctg ctggagatgc tggacgccca ccgcctacat 5101 gcgcccacta gccgtggagg ggcatccgtg gaggagacgg accaaagcca cttggccact 5161 gcgggctcta cttcatcgca ttccttgcaa aagtattaca tcacggggga ggcagagggt 5221 ttccctgcca cggtctgaga cccagctttc ttgtacaaag tggttgatat ccagcacagt 5281 ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc 5341 ctcggtctcg attctacgcg taccggttag taatgagatc cctccccccc ccctaacgtt 5401 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc 5461 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc 5521 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag 5581 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg 5641 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat 5701 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga 5761 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc 5821 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg 5881 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga 5941 tgataatatg gccacacata tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc 6001 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg 6061 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct 6121 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg 6181 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt 6241 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa 6301 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga 6361 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat 6421 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga 6481 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt 6541 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga 6601 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat 6661 ggacgagctg tacaagtaaa tcgatagatc ctaatcaacc tctggattac aaaatttgtg 6721 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 6781 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 6841 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 6901 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 6961 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 7021 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 7081 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg 7141 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 7201 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 7261 ccctttgggc cgcctccccg cctgagatcc tttaagacca atgacttaca aggcagctgt 7321 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 7381 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 7441 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 7501 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 7561 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 7621 tttataactt gcaaagaaat gaatatcaga gagtgagagg cccgggttaa ttaaggaaag 7681 ggctagatca ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg 7741 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa 7801 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 7861 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 7921 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 7981 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 8041 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 8101 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc 8161 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 8221 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 8281 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 8341 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 8401 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt 8461 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 8521 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 8581 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 8641 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 8701 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 8761 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 8821 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 8881 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 8941 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 9001 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 9061 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 9121 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 9181 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 9241 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 9301 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 9361 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 9421 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 9481 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 9541 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg 9601 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa 9661 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc 9721 ctctccccgc gcgttggccg attcattaat gcagcaagct catggctgac taattttttt 9781 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg 9841 cttttttgga ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg tttcccgact 9901 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc 9961 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat 10021 ttcacacagg aaacagctat gacatgatta cgaatttcac aaataaagca tttttttcac 10081 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact 10141 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg 10201 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt 10261 aaagaaattg tatttgttaa atatgtacta caaacttagt agt //