LOCUS Exported 9628 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION 3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Puro. ACCESSION . VERSION . KEYWORDS pLenti CMV Puro DEST (w118-1) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9628) AUTHORS Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD. TITLE A versatile viral system for expression and depletion of proteins in mammalian cells. JOURNAL PLoS One. 2009 Aug 6;4(8):e6529. PUBMED 19657394 REFERENCE 2 (bases 1 to 9628) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9628 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 95..112 /label=L4440 /note="L4440 vector, forward primer" protein_bind 229..250 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 265..295 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 303..319 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 308..330 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 327..343 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 327..343 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 362..382 /label=T3 /note="T3 promoter, forward primer" promoter 364..382 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 410..636 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 637..817 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 864..989 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1482..1715 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1900..1944 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2211..2327 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" enhancer 2350..2653 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2654..2857 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2808..2828 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 2854..2878 /label=LNCX /note="Human CMV promoter, forward primer" protein_bind 2962..3086 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 3111..3141 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 3195..3854 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(3261..3280) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 4196..4501 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 4380..4399 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(4542..4666) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" misc_feature 4697..5285 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4750..4770) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5168..5179) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" promoter 5299..5798 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(5334..5357) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 5731..5750 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 5819..6418 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(5819..5838) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6315..6335 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 6556..6789 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 6861..6982 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6898..6917) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6952..6971 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 7022..7157 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7084..7103 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(7177..7196) /label=T7 /note="T7 promoter, forward primer" promoter complement(7178..7196) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(7206..7223) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(7206..7222) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7215..7237) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 7364..7819 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7451..7470) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7661..7682 /label=F1ori-F /note="F1 origin, forward primer" promoter 7845..7949 /gene="bla" /label=AmpR promoter CDS 7950..8810 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8168..8187) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8981..9569 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9470..9489 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc 61 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga 121 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg 181 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta 241 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg 301 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg 361 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat 421 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga 481 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta 541 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga 601 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc 661 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 721 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 781 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga 841 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca 901 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct 961 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat 1021 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat 1081 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag 1141 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta 1201 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag 1261 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac 1321 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt 1381 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca 1441 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg 1501 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc 1561 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg 1621 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg 1681 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa 1741 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag 1801 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta 1861 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg 1921 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat 1981 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta 2041 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc 2101 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac 2161 agagacagat ccattcgatt agtgaacgga tctcgacggt atcggttaac ttttaaaaga 2221 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac 2281 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg 2341 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc 2401 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca 2461 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta 2521 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta 2581 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat 2641 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga 2701 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca 2761 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg 2821 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc 2881 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactct agtccagtgt 2941 ggtggaattc tgcagatatc aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat 3001 gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt 3061 aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt 3121 atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag 3181 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat 3241 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga 3301 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt 3361 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg 3421 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc 3481 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt 3541 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta 3601 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt 3661 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat 3721 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt 3781 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc 3841 agggcggggc gtaaagatct ggatccggct tactaaaagc cagataacag tatgcgtatt 3901 tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa 3961 aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt 4021 gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa 4081 gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc 4141 gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca 4201 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag 4261 tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct 4321 gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg 4381 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga 4441 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata 4501 aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt 4561 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat 4621 ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggttgat atccagcaca 4681 gtggcggccg ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat 4741 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca 4801 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc 4861 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc 4921 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt 4981 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg 5041 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc 5101 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta 5161 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg 5221 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc 5281 cccgcctgga attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg 5341 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt 5401 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt 5461 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt 5521 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat 5581 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg 5641 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg 5701 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg 5761 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat 5821 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg 5881 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg 5941 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 6001 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 6061 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 6121 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 6181 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 6241 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 6301 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 6361 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg 6421 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catctcgagg 6481 gcccggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa 6541 aagaaaaggg gggactggaa gggctagctc actcccaacg aagacaagat ctgctttttg 6601 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 6661 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 6721 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 6781 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat 6841 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca 6901 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 6961 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc 7021 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 7081 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 7141 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg 7201 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt 7261 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc 7321 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc 7381 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 7441 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 7501 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc 7561 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 7621 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 7681 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt 7741 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa 7801 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 7861 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 7921 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 7981 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 8041 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 8101 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 8161 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc 8221 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 8281 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 8341 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 8401 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 8461 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac 8521 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 8581 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 8641 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 8701 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 8761 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 8821 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 8881 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 8941 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 9001 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 9061 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 9121 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 9181 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 9241 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 9301 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 9361 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 9421 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 9481 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 9541 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 9601 tggccttttg ctggcctttt gctcacat //