LOCUS Exported 10749 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBABEpuro HA Brca1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10749) AUTHORS Cortez D, Wang Y, Qin J, Elledge SJ TITLE Requirement of ATM-dependent phosphorylation of brca1 in the DNA damage response to double-strand breaks. JOURNAL Science. 1999 Nov 5. 286(5442):1162-6. PUBMED 10550055 REFERENCE 2 (bases 1 to 10749) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10749 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(12..29) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(183..771) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(263..282) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(931..1791) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1554..1573 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1792..1896) /gene="bla" /label=AmpR promoter LTR 1927..2396 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 2461..2660 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 2861..3277 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 3207..3229 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 3245..3261 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" CDS 3348..3374 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" primer_bind complement(8998..9018) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 9003..9332 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 9183..9318 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9245..9264 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 9342..9941 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(9342..9361) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 9838..9858 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 10162..10631 /note="long terminal repeat from Moloney murine leukemia virus" ORIGIN 1 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 61 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 121 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 181 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 241 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 301 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 361 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 421 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 481 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 541 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 601 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 661 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 721 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 781 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 841 gtcatgagat tatcaaaaag gatcttcacc tagatccttt tgcggccgca aatcaatcta 901 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 961 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 1021 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 1081 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 1141 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 1201 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 1261 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 1321 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 1381 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 1441 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 1501 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 1561 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 1621 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 1681 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 1741 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 1801 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 1861 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 1921 tgcagcctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag 1981 ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt 2041 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt 2101 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct 2161 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag 2221 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg 2281 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct 2341 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatttg 2401 ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc accgggaggc 2461 aagctggcca gcaacttatc tgtgtctgtc cgattgtcta gtgtctatga ctgattttat 2521 gcgcctgcgt cggtactagt tagctaacta gctctgtatc tggcggaccc gtggtggaac 2581 tgacgagttc tgaacacccg gccgcaaccc tgggagacgt cccagggact ttgggggccg 2641 tttttgtggc ccgacctgag gaagggagtc gatgtggaat ccgaccccgt caggatatgt 2701 ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc 2761 ggtttggaac cgaagccgcg cgtcttgtct gctgcagcgc tgcagcatcg ttctgtgttg 2821 tctctgtctg actgtgtttc tgtatttgtc tgaaaattag ggccagactg ttaccactcc 2881 cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt 2941 agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt 3001 cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt 3061 cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc 3121 cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc 3181 tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc 3241 ctccctttat ccagccctca ctccttctct aggcgccggc cggatcccag tgtggtggta 3301 cgtaggaatt cgccagcaca gtggtcgacc ccaagcttac catggcctac ccctacgacg 3361 tgcccgacta cgcctccctc ggatccatgg atttatctgc tcttcgcgtt gaagaagtac 3421 aaaatgtcat taatgctatg cagaaaatct tagagtgtcc catctgtctg gagttgatca 3481 aggaacctgt ctccacaaag tgtgaccaca tattttgcaa attttgcatg ctgaaacttc 3541 tcaaccagaa gaaagggcct tcacagtgtc ctttatgtaa gaatgatata accaaaagga 3601 gcctacaaga aagtacgaga tttagtcaac ttgttgaaga gctattgaaa atcatttgtg 3661 cttttcagct tgacacaggt ttggagtatg caaacagcta taattttgca aaaaaggaaa 3721 ataactctcc tgaacatcta aaagatgaag tttctatcat ccaaagtatg ggctacagaa 3781 accgtgccaa aagacttcta cagagtgaac ccgaaaatcc ttccttgcag gaaaccagtc 3841 tcagtgtcca actctctaac cttggaactg tgagaactct gaggacaaag cagcggatac 3901 aacctcaaaa gacgtctgtc tacattgaat tgggatctga ttcttctgaa gataccgtta 3961 ataaggcaac ttattgcagt gtgggagatc aagaattgtt acaaatcacc cctcaaggaa 4021 ccagggatga aatcagtttg gattctgcaa aaaaggctgc ttgtgaattt tctgagacgg 4081 atgtaacaaa tactgaacat catcaaccca gtaataatga tttgaacacc actgagaagc 4141 gtgcagctga gaggcatcca gaaaagtatc agggtagttc tgtttcaaac ttgcatgtgg 4201 agccatgtgg cacaaatact catgccagct cattacagca tgagaacagc agtttattac 4261 tcactaaaga cagaatgaat gtagaaaagg ctgaattctg taataaaagc aaacagcctg 4321 gcttagcaag gagccaacat aacagatggg ctggaagtaa ggaaacatgt aatgataggc 4381 ggactcccag cacagaaaaa aaggtagatc tgaatgctga tcccctgtgt gagagaaaag 4441 aatggaataa gcagaaactg ccatgctcag agaatcctag agatactgaa gatgttcctt 4501 ggataacact aaatagcagc attcagaaag ttaatgagtg gttttccaga agtgatgaac 4561 tgttaggttc tgatgactca catgatgggg agtctgaatc aaatgccaaa gtagctgatg 4621 tattggacgt tctaaatgag gtagatgaat attctggttc ttcagagaaa atagacttac 4681 tggccagtga tcctcatgag gctttaatat gtaaaagtga aagagttcac tccaaatcag 4741 tagagagtaa tattgaagac aaaatatttg ggaaaaccta tcggaagaag gcaagcctcc 4801 ccaacttaag ccatgtaact gaaaatctaa ttataggagc atttgttact gagccacaga 4861 taatacaaga gcgtcccctc acaaataaat taaagcgtaa aaggagacct acatcaggcc 4921 ttcatcctga ggattttatc aagaaagcag atttggcagt tcaaaagact cctgaaatga 4981 taaatcaggg aactaaccaa acggagcaga atggtcaagt gatgaatatt actaatagtg 5041 gtcatgagaa taaaacaaaa ggtgattcta ttcagaatga gaaaaatcct aacccaatag 5101 aatcactcga aaaagaatct gctttcaaaa cgaaagctga acctataagc agcagtataa 5161 gcaatatgga actcgaatta aatatccaca attcaaaagc acctaaaaag aataggctga 5221 ggaggaagtc ttctaccagg catattcatg cgcttgaact agtagtcagt agaaatctaa 5281 gcccacctaa ttgtactgaa ttgcaaattg atagttgttc tagcagtgaa gagataaaga 5341 aaaaaaagta caaccaaatg ccagtcaggc acagcagaaa cctacaactc atggaaggta 5401 aagaacctgc aactggagcc aagaagagta acaagccaaa tgaacagaca agtaaaagac 5461 atgacagtga tactttccca gagctgaagt taacaaatgc acctggttct tttactaagt 5521 gttcaaatac cagtgaactt aaagaatttg tcaatcctag ccttccaaga gaagaaaaag 5581 aagagaaact agaaacagtt aaagtgtcta ataatgctga agaccccaaa gatctcatgt 5641 taagtggaga aagggttttg caaactgaaa gatctgtaga gagtagcagt atttcactgg 5701 tacctggtac tgattatggc actcaggaaa gtatctcgtt actggaagtt agcactctag 5761 ggaaggcaaa aacagaacca aataaatgtg tgagtcagtg tgcagcattt gaaaacccca 5821 agggactaat tcatggttgt tccaaagata atagaaatga cacagaaggc tttaagtatc 5881 cattgggaca tgaagttaac cacagtcggg aaacaagcat agaaatggaa gaaagtgaac 5941 ttgatgctca gtatttgcag aatacattca aggtttcaaa gcgccagtca tttgctctgt 6001 tttcaaatcc aggaaatgca gaagaggaat gtgcaacatt ctctgcccac tctgggtcct 6061 taaagaaaca aagtccaaaa gtcacttttg aatgtgaaca aaaggaagaa aatcaaggaa 6121 agaatgagtc taatatcaag cctgtacaga cagttaatat cactgcaggc tttcctgtgg 6181 ttggtcagaa agataagcca gttgataatg ccaaatgtag tatcaaagga ggctctaggt 6241 tttgtctatc atctcagttc agaggcaacg aaactggact cattactcca aataaacatg 6301 gacttttaca aaacccatat cgtataccac cactttttcc catcaagtca tttgttaaaa 6361 ctaaatgtaa gaaaaatctg ctagaggaaa actttgagga acattcaatg tcacctgaaa 6421 gagaaatggg aaatgagaac attccaagta cagtgagcac aattagccgt aataacatta 6481 gagaaaatgt ttttaaagga gccagctcaa gcaatattaa tgaagtaggt tccagtacta 6541 atgaagtggg ctccagtatt aatgaaatag gttccagtga tgaaaacatt caagcagaac 6601 taggtagaaa cagagggcca aaattgaatg ctatgcttag attaggggtt ttgcaacctg 6661 aggtctataa acaaagtctt cctggaagta attgtaagca tcctgaaata aaaaagcaag 6721 aatatgaaga agtagttcag actgttaata cagatttctc tccatatctg atttcagata 6781 acttagaaca gcctatggga agtagtcatg catctcaggt ttgttctgag acacctgatg 6841 acctgttaga tgatggtgaa ataaaggaag atactagttt tgctgaaaat gacattaagg 6901 aaagttctgc tgtttttagc aaaagcgtcc agagaggaga gcttagcagg agtcctagcc 6961 ctttcaccca tacacatttg gctcagggtt accgaagagg ggccaagaaa ttagagtcct 7021 cagaagagaa cttatctagt gaggatgaag agcttccctg cttccaacac ttgttatttg 7081 gtaaagtaaa caatatacct tctcagtcta ctaggcatag caccgttgct accgagtgtc 7141 tgtctaagaa cacagaggag aatttattat cattgaagaa tagcttaaat gactgcagta 7201 accaggtaat attggcaaag gcatctcagg aacatcacct tagtgaggaa acaaaatgtt 7261 ctgctagctt gttttcttca cagtgcagtg aattggaaga cttgactgca aatacaaaca 7321 cccaggatcc tttcttgatt ggttcttcca aacaaatgag gcatcagtct gaaagccagg 7381 gagttggtct gagtgacaag gaattggttt cagatgatga agaaagagga acgggcttgg 7441 aagaaaataa tcaagaagag caaagcatgg attcaaactt aggtgaagca gcatctgggt 7501 gtgagagtga aacaagcgtc tctgaagact gctcagggct atcctctcag agtgacattt 7561 taaccactca gcagagggat accatgcaac ataacctgat aaagctccag caggaaatgg 7621 ctgaactaga agctgtgtta gaacagcatg ggagccagcc ttctaacagc tacccttcca 7681 tcataagtga ctcctctgcc cttgaggacc tgcgaaatcc agaacaaagc acatcagaaa 7741 aagcagtatt aacttcacag aaaagtagtg aataccctat aagccagaat ccagaaggcc 7801 tttctgctga caagtttgag gtgtctgcag atagttctac cagtaaaaat aaagaaccag 7861 gagtggaaag gtcatcccct tctaaatgcc catcattaga tgataggtgg tacatgcaca 7921 gttgctctgg gagtcttcag aatagaaact acccatctca agaggagctc attaaggttg 7981 ttgatgtgga ggagcaacag ctggaagagt ctgggccaca cgatttgacg gaaacatctt 8041 acttgccaag gcaagatcta gagggaaccc cttacctgga atctggaatc agcctcttct 8101 ctgatgaccc tgaatctgat ccttctgaag acagagcccc agagtcagct cgtgttggca 8161 acataccatc ttcaacctct gcattgaaag ttccccaatt gaaagttgca gaatctgccc 8221 agggtccagc tgctgctcat actactgata ctgctgggta taatgcaatg gaagaaagtg 8281 tgagcaggga gaagccagaa ttgacagctt caacagaaag ggtcaacaaa agaatgtcca 8341 tggtggtgtc tggcctgacc ccagaagaat ttatgctcgt gtacaagttt gccagaaaac 8401 accacatcac tttaactaat ctaattactg aagagactac tcatgttgtt atgaaaacag 8461 atgctgagtt tgtgtgtgaa cggacactga aatattttct aggaattgcg ggaggaaaat 8521 gggtagttag ctatttctgg gtgacccagt ctattaaaga aagaaaaatg ctgaatgagc 8581 atgattttga agtcagagga gatgtggtca atggaagaaa ccaccaaggt ccaaagcgag 8641 caagagaatc ccaggacaga aagatcttca gggggctaga aatctgttgc tatgggccct 8701 tcaccaacat gcccacagat caactggaat ggatggtaca gctgtgtggt gcttctgtgg 8761 tgaaggagct ttcatcattc acccttggca caggtgtcca cccaattgtg gttgtgcagc 8821 cagatgcctg gacagaggac aatggcttcc atgcaattgg gcagatgtgt gaggcacctg 8881 tggtgacccg agagtgggtg ttggacagtg tagcactcta ccagtgccag gagctggaca 8941 cctacctgat accccagatc ccccacagcc actactgaga tatcggctcg accctgtgga 9001 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 9061 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 9121 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 9181 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 9241 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 9301 gaggcttttt tggaggccta ggcttttgca aaaagcttac catgaccgag tacaagccca 9361 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt 9421 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca 9481 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg 9541 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg 9601 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc 9661 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca 9721 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg 9781 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca 9841 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag 9901 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc 9961 agcgcccgac cgaaaggagc gcacgacccc atgcatcgat aaaataaaag attttattta 10021 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag 10081 taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag ttcagatcaa 10141 ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt 10201 cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca aacaggatat 10261 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt 10321 ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga 10381 aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg 10441 cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc gccagtcctc 10501 cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg 10561 acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg 10621 ggggtctttc acatgcagca tgtatcaaaa ttaatttggt tttttttctt aagtatttac 10681 attaaatggc catagttgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 10741 attgggcgc //