LOCUS Exported 9604 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of PTEN R233*. ACCESSION . VERSION . KEYWORDS pHAGE-PTEN-R233* SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9604) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 9604) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9604 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(4661..4685) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 4738..4779 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 4800..5367 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 4817..5369 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(4961..4978) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5188..5207 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5381..5980 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(5381..5400) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 5877..5897 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 5994..6582 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6047..6067) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6465..6476) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6657..6890 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7009..7027) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7095..7199 /gene="bla" /label=AmpR promoter CDS 7200..8060 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7418..7437) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8231..8819 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8720..8739 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8973..8990 /label=L4440 /note="L4440 vector, forward primer" primer_bind 9082..9101 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 9230..9251 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9266..9296 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9304..9320 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9309..9331 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9328..9344 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9328..9344 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 9394..9413 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcaccatg acagccatca tcaaagagat cgttagcaga 3481 aacaaaagga gatatcaaga ggatggattc gacttagact tgacctatat ttatccaaac 3541 attattgcta tgggatttcc tgcagaaaga cttgaaggcg tatacaggaa caatattgat 3601 gatgtagtaa ggtttttgga ttcaaagcat aaaaaccatt acaagatata caatctttgt 3661 gctgaaagac attatgacac cgccaaattt aattgcagag ttgcacaata tccttttgaa 3721 gaccataacc caccacagct agaacttatc aaaccctttt gtgaagatct tgaccaatgg 3781 ctaagtgaag atgacaatca tgttgcagca attcactgta aagctggaaa gggacgaact 3841 ggtgtaatga tatgtgcata tttattacat cggggcaaat ttttaaaggc acaagaggcc 3901 ctagatttct atggggaagt aaggaccaga gacaaaaagg gagtaactat tcccagtcag 3961 aggcgctatg tgtattatta tagctacctg ttaaagaatc atctggatta tagaccagtg 4021 gcactgttgt ttcacaagat gatgtttgaa actattccaa tgttcagtgg cggaacttgc 4081 aatcctcagt ttgtggtctg ccagctaaag gtgaagatat attcctccaa ttcaggaccc 4141 acatgacggg aagacaagtt catgtacttt gagttccctc agccgttacc tgtgtgtggt 4201 gatatcaaag tagagttctt ccacaaacag aacaagatgc taaaaaagga caaaatgttt 4261 cacttttggg taaatacatt cttcatacca ggaccagagg aaacctcaga aaaagtagaa 4321 aatggaagtc tatgtgatca agaaatcgat agcatttgca gtatagagcg tgcagataat 4381 gacaaggaat atctagtact tactttaaca aaaaatgatc ttgacaaagc aaataaagac 4441 aaagccaacc gatacttttc tccaaatttt aaggtgaagc tgtacttcac aaaaacagta 4501 gaggagccgt caaatccaga ggctagcagt tcaacttctg taacaccaga tgttagtgac 4561 aatgaacctg atcattatag atattctgac accactgact ctgatccaga gaatgaacct 4621 tttgatgaag atcagcatac acaaattaca aaagtctaga acccagcttt cttgtacaaa 4681 gtggttgata tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt 4741 aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtta gtaatgagat 4801 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt 4861 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct 4921 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa 4981 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg 5041 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc 5101 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg 5161 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg 5221 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc 5281 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg 5341 gttttccttt gaaaaacacg atgataatat ggccacacat atgaccgagt acaagcccac 5401 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt 5461 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac 5521 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc 5581 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt 5641 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca 5701 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac 5761 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg 5821 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa 5881 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg 5941 accgcgcacc tggtgcatga cccgcaagcc cggtgcctaa atcgatagat cctaatcaac 6001 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta 6061 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 6121 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg 6181 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg 6241 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca 6301 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca 6361 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg 6421 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag 6481 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc 6541 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc ctttaagacc 6601 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga 6661 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg 6721 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 6781 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 6841 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 6901 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 6961 gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac 7021 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt 7081 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 7141 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 7201 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 7261 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 7321 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 7381 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 7441 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 7501 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 7561 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 7621 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 7681 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 7741 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 7801 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 7861 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 7921 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 7981 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 8041 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt 8101 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 8161 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 8221 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 8281 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 8341 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag 8401 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 8461 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 8521 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 8581 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 8641 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 8701 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 8761 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 8821 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 8881 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg 8941 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag 9001 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc 9061 tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 9121 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt 9181 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt 9241 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg 9301 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca 9361 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 9421 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca 9481 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt 9541 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag 9601 tagt //