LOCUS Exported 9872 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of PTPN11 E139D. ACCESSION . VERSION . KEYWORDS pHAGE-PTPN11-E139D SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9872) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 9872) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9872 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(4832..4856) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 4864..4882 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(4907..4926) /label=T7 /note="T7 promoter, forward primer" promoter complement(4908..4926) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(4932..4952) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 5006..5047 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 5068..5635 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 5085..5637 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5229..5246) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5456..5475 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5649..6248 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(5649..5668) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6145..6165 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 6262..6850 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6315..6335) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6733..6744) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6925..7158 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7277..7295) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7363..7467 /gene="bla" /label=AmpR promoter CDS 7468..8328 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7686..7705) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8499..9087 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8988..9007 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9241..9258 /label=L4440 /note="L4440 vector, forward primer" primer_bind 9350..9369 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 9498..9519 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9534..9564 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9572..9588 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9577..9599 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9596..9612 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9596..9612 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 9662..9681 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcaccatg acatcgcgga gatggtttca cccaaatatc 3481 actggtgtgg aggcagaaaa cctactgttg acaagaggag ttgatggcag ttttttggca 3541 aggcctagta aaagtaaccc tggagacttc acactttccg ttagaagaaa tggagctgtc 3601 acccacatca agattcagaa cactggtgat tactatgacc tgtatggagg ggagaaattt 3661 gccactttgg ctgagttggt ccagtattac atggaacatc acgggcaatt aaaagagaag 3721 aatggagatg tcattgagct taaatatcct ctgaactgtg cagatcctac ctctgaaagg 3781 tggtttcatg gacatctctc tgggaaagaa gcagagaaat tattaactga aaaaggaaaa 3841 catggtagtt ttcttgtacg agacagccag agccaccctg gagattttgt tctttctgtg 3901 cgcactggtg atgacaaagg ggagagcaat gacggcaagt ctaaagtgac ccatgttatg 3961 attcgctgtc aggaactgaa atacgacgtt ggtggaggag aacggtttga ttctttgaca 4021 gatcttgtgg aacattataa gaagaatcct atggtggaaa cattgggtac agtactacaa 4081 ctcaagcagc cccttaacac gactcgtata aatgctgctg aaatagaaag cagagttcga 4141 gaactaagca aattagctga gaccacagat aaagtcaaac aaggcttttg ggaagaattt 4201 gagacactac aacaacagga gtgcaaactt ctctacagcc gaaaagaggg tcaaaggcaa 4261 gaaaacaaaa acaaaaatag atataaaaac atcctgccct ttgatcatac cagggttgtc 4321 ctacacgatg gtgatcccaa tgagcctgtt tcagattaca tcaatgcaaa tatcatcatg 4381 cctgaatttg aaaccaagtg caacaattca aagcccaaaa agagttacat tgccacacaa 4441 ggctgcctgc aaaacacggt gaatgacttt tggcggatgg tgttccaaga aaactcccga 4501 gtgattgtca tgacaacgaa agaagtggag agaggaaaga gtaaatgtgt caaatactgg 4561 cctgatgagt atgctctaaa agaatatggc gtcatgcgtg ttaggaacgt caaagaaagc 4621 gccgctcatg actatacgct aagagaactt aaactttcaa aggttggaca agggaatacg 4681 gagagaacgg tctggcaata ccactttcgg acctggccgg accacggcgt gcccagcgac 4741 cctgggggcg tgctggactt cctggaggag gtgcaccata agcaggagag catcatggat 4801 gcagggccgg tcgtggtgca ctgcaggtag aacccagctt tcttgtacaa agtggtgata 4861 tccaattaac cctcactaaa ggtattagag agaaattgta gagattccct atagtgagtc 4921 gtattaccac ccaacttttc tatacaaagt ggttgatatc cagcacagtg gcggccgctc 4981 gagtctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga 5041 ttctacgcgt accggttagt aatgagatcc ctcccccccc cctaacgtta ctggccgaag 5101 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc 5161 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg 5221 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc 5281 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc 5341 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa 5401 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct 5461 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg 5521 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg 5581 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg 5641 ccacacatat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca 5701 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg 5761 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg 5821 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca 5881 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt 5941 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc 6001 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg 6061 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg 6121 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg 6181 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg 6241 gtgcctaaat cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact 6301 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 6361 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg 6421 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg 6481 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg 6541 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc 6601 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca 6661 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc 6721 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct 6781 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc 6841 gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc 6901 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc 6961 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg 7021 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag 7081 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag 7141 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg 7201 caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat 7261 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata 7321 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 7381 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 7441 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 7501 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 7561 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 7621 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 7681 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc 7741 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 7801 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 7861 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 7921 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 7981 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 8041 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 8101 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 8161 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 8221 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 8281 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 8341 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 8401 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 8461 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 8521 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 8581 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 8641 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 8701 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 8761 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 8821 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 8881 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 8941 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 9001 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 9061 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 9121 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 9181 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 9241 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 9301 cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt atttatgcag 9361 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 9421 gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc 9481 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac 9541 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga 9601 aacagctatg acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt 9661 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa 9721 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg 9781 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt 9841 atttgttaaa tatgtactac aaacttagta gt //