LOCUS Exported 10436 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of RAF1 L86R. ACCESSION . VERSION . KEYWORDS pHAGE-RAF1-L86R SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10436) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 10436) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10436 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(5396..5420) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 5428..5446 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(5471..5490) /label=T7 /note="T7 promoter, forward primer" promoter complement(5472..5490) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(5496..5516) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 5570..5611 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 5632..6199 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 5649..6201 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5793..5810) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 6020..6039 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 6213..6812 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(6213..6232) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6709..6729 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 6826..7414 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6879..6899) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7297..7308) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7489..7722 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7841..7859) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7927..8031 /gene="bla" /label=AmpR promoter CDS 8032..8892 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8250..8269) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9063..9651 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9552..9571 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9805..9822 /label=L4440 /note="L4440 vector, forward primer" primer_bind 9914..9933 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 10062..10083 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 10098..10128 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10136..10152 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 10141..10163 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 10160..10176 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 10160..10176 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 10226..10245 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcaccatg gagcacatac agggagcttg gaagacgatc 3481 agcaatggtt ttggattcaa agatgccgtg tttgatggct ccagctgcat ctctcctaca 3541 atagttcagc agtttggcta tcagcgccgg gcatcagatg atggcaaact cacagatcct 3601 tctaagacaa gcaacactat ccgtgttttc ttgccgaaca agcaaagaac agtggtcaat 3661 gtgcgaaatg gaatgagctt gcatgactgc cttatgaaag cacgcaaggt gaggggcctg 3721 caaccagagt gctgtgcagt gttcagactt ctccacgaac acaaaggtaa aaaagcacgc 3781 ttagattgga atactgatgc tgcgtctttg attggagaag aacttcaagt agatttcctg 3841 gatcatgttc ccctcacaac acacaacttt gctcggaaga cgttcctgaa gcttgccttc 3901 tgtgacatct gtcagaaatt cctgctcaat ggatttcgat gtcagacttg tggctacaaa 3961 tttcatgagc actgtagcac caaagtacct actatgtgtg tggactggag taacatcaga 4021 caactcttat tgtttccaaa ttccactatt ggtgatagtg gagtcccagc actaccttct 4081 ttgactatgc gtcgtatgcg agagtctgtt tccaggatgc ctgttagttc tcagcacaga 4141 tattctacac ctcacgcctt cacctttaac acctccagtc cctcatctga aggttccctc 4201 tcccagaggc agaggtcgac atccacacct aatgtccaca tggtcagcac caccctgcct 4261 gtggacagca ggatgattga ggatgcaatt cgaagtcaca gcgaatcagc ctcaccttca 4321 gccctgtcca gtagccccaa caatctgagc ccaacaggct ggtcacagcc gaaaaccccc 4381 gtgccagcac aaagagagcg ggcaccagta tctgggaccc aggagaaaaa caaaattagg 4441 cctcgtggac agagagattc aagctattat tgggaaatag aagccagtga agtgatgctg 4501 tccactcgga ttgggtcagg ctcttttgga actgtttata agggtaaatg gcacggagat 4561 gttgcagtaa agatcctaaa ggttgtcgac ccaaccccag agcaattcca ggccttcagg 4621 aatgaggtgg ctgttctgcg caaaacacgg catgtgaaca ttctgctttt catggggtac 4681 atgacaaagg acaacctggc aattgtgacc cagtggtgcg agggcagcag cctctacaaa 4741 cacctgcatg tccaggagac caagtttcag atgttccagc taattgacat tgcccggcag 4801 acggctcagg gaatggacta tttgcatgca aagaacatca tccatagaga catgaaatcc 4861 aacaatatat ttctccatga aggcttaaca gtgaaaattg gagattttgg tttggcaaca 4921 gtaaagtcac gctggagtgg ttctcagcag gttgaacaac ctactggctc tgtcctctgg 4981 atggccccag aggtgatccg aatgcaggat aacaacccat tcagtttcca gtcggatgtc 5041 tactcctatg gcatcgtatt gtatgaactg atgacggggg agcttcctta ttctcacatc 5101 aacaaccgag atcagatcat cttcatggtg ggccgaggat atgcctcccc agatcttagt 5161 aagctatata agaactgccc caaagcaatg aagaggctgg tagctgactg tgtgaagaaa 5221 gtaaaggaag agaggcctct ttttccccag atcctgtctt ccattgagct gctccaacac 5281 tctctaccga agatcaaccg gagcgcttcc gagccatcct tgcatcgggc agcccacact 5341 gaggatatca atgcttgcac gctgaccacg tccccgaggc tgcctgtctt ctagaaccca 5401 gctttcttgt acaaagtggt gatatccaat taaccctcac taaaggaaga tgatagttaa 5461 gtgtaagtta ccctatagtg agtcgtatta ccacccaact tttctataca aagtggttga 5521 tatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat 5581 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt tagtaatgag atccctcccc 5641 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat 5701 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt 5761 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt 5821 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc 5881 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc 5941 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat 6001 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc 6061 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg 6121 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct 6181 ttgaaaaaca cgatgataat atggccacac atatgaccga gtacaagccc acggtgcgcc 6241 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact 6301 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc 6361 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 6421 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 6481 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 6541 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 6601 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 6661 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 6721 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca 6781 cctggtgcat gacccgcaag cccggtgcct aaatcgatag atcctaatca acctctggat 6841 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 6901 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 6961 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 7021 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 7081 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 7141 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 7201 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc 7261 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 7321 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag 7381 acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga ccaatgactt 7441 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa 7501 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc 7561 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 7621 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 7681 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc 7741 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggcccgggt 7801 taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt 7861 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga 7921 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 7981 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 8041 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 8101 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 8161 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 8221 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac 8281 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 8341 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 8401 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 8461 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 8521 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 8581 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 8641 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 8701 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 8761 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 8821 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 8881 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 8941 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 9001 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 9061 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 9121 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 9181 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 9241 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 9301 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 9361 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 9421 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 9481 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 9541 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 9601 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 9661 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 9721 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 9781 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca 9841 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct 9901 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga 9961 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc gtggcacgac 10021 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 10081 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 10141 agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt cacaaataaa 10201 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 10261 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac 10321 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa 10381 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt //