LOCUS Exported 10459 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of RAF1 S257L. ACCESSION . VERSION . KEYWORDS pHAGE-RAF1-S257L SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10459) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 10459) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10459 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(5396..5420) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 5473..5514 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 5535..6102 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 5552..6104 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5696..5713) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5923..5942 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 6116..6835 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 6116..6832 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(6161..6182) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6422..6441) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6769..6790 /label=EGFP-C /note="EGFP, forward primer" misc_feature 6849..7437 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6902..6922) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7320..7331) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7512..7745 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7864..7882) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7950..8054 /gene="bla" /label=AmpR promoter CDS 8055..8915 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8273..8292) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9086..9674 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9575..9594 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9828..9845 /label=L4440 /note="L4440 vector, forward primer" primer_bind 9937..9956 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 10085..10106 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 10121..10151 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10159..10175 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 10164..10186 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 10183..10199 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 10183..10199 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 10249..10268 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcaccatg gagcacatac agggagcttg gaagacgatc 3481 agcaatggtt ttggattcaa agatgccgtg tttgatggct ccagctgcat ctctcctaca 3541 atagttcagc agtttggcta tcagcgccgg gcatcagatg atggcaaact cacagatcct 3601 tctaagacaa gcaacactat ccgtgttttc ttgccgaaca agcaaagaac agtggtcaat 3661 gtgcgaaatg gaatgagctt gcatgactgc cttatgaaag cactcaaggt gaggggcctg 3721 caaccagagt gctgtgcagt gttcagactt ctccacgaac acaaaggtaa aaaagcacgc 3781 ttagattgga atactgatgc tgcgtctttg attggagaag aacttcaagt agatttcctg 3841 gatcatgttc ccctcacaac acacaacttt gctcggaaga cgttcctgaa gcttgccttc 3901 tgtgacatct gtcagaaatt cctgctcaat ggatttcgat gtcagacttg tggctacaaa 3961 tttcatgagc actgtagcac caaagtacct actatgtgtg tggactggag taacatcaga 4021 caactcttat tgtttccaaa ttccactatt ggtgatagtg gagtcccagc actaccttct 4081 ttgactatgc gtcgtatgcg agagtctgtt tccaggatgc ctgttagttc tcagcacaga 4141 tattctacac ctcacgcctt cacctttaac acctccagtc cctcatctga aggttccctc 4201 tcccagaggc agaggttgac atccacacct aatgtccaca tggtcagcac caccctgcct 4261 gtggacagca ggatgattga ggatgcaatt cgaagtcaca gcgaatcagc ctcaccttca 4321 gccctgtcca gtagccccaa caatctgagc ccaacaggct ggtcacagcc gaaaaccccc 4381 gtgccagcac aaagagagcg ggcaccagta tctgggaccc aggagaaaaa caaaattagg 4441 cctcgtggac agagagattc aagctattat tgggaaatag aagccagtga agtgatgctg 4501 tccactcgga ttgggtcagg ctcttttgga actgtttata agggtaaatg gcacggagat 4561 gttgcagtaa agatcctaaa ggttgtcgac ccaaccccag agcaattcca ggccttcagg 4621 aatgaggtgg ctgttctgcg caaaacacgg catgtgaaca ttctgctttt catggggtac 4681 atgacaaagg acaacctggc aattgtgacc cagtggtgcg agggcagcag cctctacaaa 4741 cacctgcatg tccaggagac caagtttcag atgttccagc taattgacat tgcccggcag 4801 acggctcagg gaatggacta tttgcatgca aagaacatca tccatagaga catgaaatcc 4861 aacaatatat ttctccatga aggcttaaca gtgaaaattg gagattttgg tttggcaaca 4921 gtaaagtcac gctggagtgg ttctcagcag gttgaacaac ctactggctc tgtcctctgg 4981 atggccccag aggtgatccg aatgcaggat aacaacccat tcagtttcca gtcggatgtc 5041 tactcctatg gcatcgtatt gtatgaactg atgacggggg agcttcctta ttctcacatc 5101 aacaaccgag atcagatcat cttcatggtg ggccgaggat atgcctcccc agatcttagt 5161 aagctatata agaactgccc caaagcaatg aagaggctgg tagctgactg tgtgaagaaa 5221 gtaaaggaag agaggcctct ttttccccag atcctgtctt ccattgagct gctccaacac 5281 tctctaccga agatcaaccg gagcgcttcc gagccatcct tgcatcgggc agcccacact 5341 gaggatatca atgcttgcac gctgaccacg tccccgaggc tgcctgtctt ctagaaccca 5401 gctttcttgt acaaagtggt tgatatccag cacagtggcg gccgctcgag tctagagggc 5461 ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc 5521 ggttagtaat gagatccctc ccccccccct aacgttactg gccgaagccg cttggaataa 5581 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg 5641 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc 5701 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct 5761 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac 5821 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc 5881 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta 5941 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg 6001 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg 6061 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca cacatatggt 6121 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 6181 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 6241 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 6301 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca 6361 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 6421 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 6481 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 6541 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 6601 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 6661 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 6721 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 6781 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaatcga 6841 tagatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact 6901 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg 6961 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg 7021 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa 7081 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc 7141 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg 7201 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt 7261 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt 7321 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc 7381 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg 7441 agatccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga 7501 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg 7561 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 7621 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 7681 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 7741 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat 7801 atcagagagt gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa 7861 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga 7921 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 7981 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 8041 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg 8101 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag 8161 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg 8221 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg 8281 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt 8341 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga 8401 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac 8461 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc 8521 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc 8581 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac 8641 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag 8701 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 8761 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 8821 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg 8881 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 8941 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 9001 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 9061 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 9121 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 9181 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 9241 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 9301 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 9361 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 9421 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 9481 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 9541 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 9601 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 9661 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 9721 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 9781 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 9841 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 9901 attaatgcag caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 9961 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 10021 caaaaagctc cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg 10081 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg 10141 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca 10201 tgattacgaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 10261 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc 10321 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac 10381 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat 10441 gtactacaaa cttagtagt //