LOCUS Exported 9623 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt. ACCESSION . VERSION . KEYWORDS pFH52 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9623) AUTHORS Hahn F, Korolev A, Sanjurjo Loures L, Nekrasov V TITLE A modular cloning toolkit for genome editing in plants. JOURNAL BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. PUBMED 32326901 REFERENCE 2 (bases 1 to 9623) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9623 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 438..783 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" primer_bind 726..746 /label=35S promoter /note="CaMV 35S promoter, forward primer" CDS 874..4977 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 4990..5010 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" terminator 5026..5278 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" misc_feature 5326..5350 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS complement(5506..6654) /codon_start=1 /product="trans-acting replication protein that binds to and activates oriV" /label=trfA /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR" rep_origin complement(6990..7578) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7070..7089) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7749..8609) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8372..8391 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8610..8714) /gene="bla" /label=AmpR promoter rep_origin 8801..9432 /label=oriV /note="incP origin of replication" misc_feature 9524..9548 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" ORIGIN 1 tctgtgaaga caagcaagaa ttcaagcttg gaggtcaaca tggtggagca cgacactctg 61 gtctactcca aaaatgtcaa agatacagtc tcagaagatc aaagggctat tgagactttt 121 caacaaagga taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc 181 atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga 241 aaggctatca ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg 301 aggagcatcg tggaaaaaga agaggttcca accacgtcta caaagcaagt ggattgatgt 361 gataacatgg tggagcacga cactctggtc tactccaaaa atgtcaaaga tacagtctca 421 gaagatcaaa gggctattga gacttttcaa caaaggataa tttcgggaaa cctcctcgga 481 ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga aggtggctcc 541 tacaaatgcc atcattgcga taaaggaaag gctatcattc aagatctctc tgccgacagt 601 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga ggttccaacc 661 acgtctacaa agcaagtgga ttgatgtgac atctccactg acgtaaggga tgacgcacaa 721 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg 781 acacgctcga gtataagagc tcatttttac aacaattacc aacaacaaca aacaacaaac 841 aacattacaa ttacatttac aattatcgat acaatggata agaagtactc tatcggactc 901 gatatcggaa ctaactctgt gggatgggct gtgatcaccg atgagtacaa ggtgccatct 961 aagaagttca aggttctcgg aaacaccgat aggcactcta tcaagaaaaa ccttatcggt 1021 gctctcctct tcgattctgg tgaaactgct gaggctacca gactcaagag aaccgctaga 1081 agaaggtaca ccagaagaaa gaacaggatc tgctacctcc aagagatctt ctctaacgag 1141 atggctaaag tggatgattc attcttccac aggctcgaag agtcattcct cgtggaagaa 1201 gataagaagc acgagaggca ccctatcttc ggaaacatcg ttgatgaggt ggcataccac 1261 gagaagtacc ctactatcta ccacctcaga aagaagctcg ttgattctac tgataaggct 1321 gatctcaggc tcatctacct cgctctcgct cacatgatca agttcagagg acacttcctc 1381 atcgagggtg atctcaaccc tgataactct gatgtggata agttgttcat ccagctcgtg 1441 cagacctaca accagctttt cgaagagaac cctatcaacg cttcaggtgt ggatgctaag 1501 gctatcctct ctgctaggct ctctaagtca agaaggcttg agaacctcat tgctcagctc 1561 cctggtgaga agaagaacgg acttttcgga aacttgatcg ctctctctct cggactcacc 1621 cctaacttca agtctaactt cgatctcgct gaggatgcaa agctccagct ctcaaaggat 1681 acctacgatg atgatctcga taacctcctc gctcagatcg gagatcagta cgctgatttg 1741 ttcctcgctg ctaagaacct ctctgatgct atcctcctca gtgatatcct cagagtgaac 1801 accgagatca ccaaggctcc actctcagct tctatgatca agagatacga tgagcaccac 1861 caggatctca cacttctcaa ggctcttgtt agacagcagc tcccagagaa gtacaaagag 1921 attttcttcg atcagtctaa gaacggatac gctggttaca tcgatggtgg tgcatctcaa 1981 gaagagttct acaagttcat caagcctatc ctcgagaaga tggatggaac cgaggaactc 2041 ctcgtgaagc tcaatagaga ggatcttctc agaaagcaga ggaccttcga taacggatct 2101 atccctcatc agatccacct cggagagttg cacgctatcc ttagaaggca agaggatttc 2161 tacccattcc tcaaggataa cagggaaaag attgagaaga ttctcacctt cagaatccct 2221 tactacgtgg gacctctcgc tagaggaaac tcaagattcg cttggatgac cagaaagtct 2281 gaggaaacca tcaccccttg gaacttcgaa gaggtggtgg ataagggtgc tagtgctcag 2341 tctttcatcg agaggatgac caacttcgat aagaaccttc caaacgagaa ggtgctccct 2401 aagcactctt tgctctacga gtacttcacc gtgtacaacg agttgaccaa ggttaagtac 2461 gtgaccgagg gaatgaggaa gcctgctttt ttgtcaggtg agcaaaagaa ggctatcgtt 2521 gatctcttgt tcaagaccaa cagaaaggtg accgtgaagc agctcaaaga ggattacttc 2581 aagaaaatcg agtgcttcga ttcagttgag atttctggtg ttgaggatag gttcaacgca 2641 tctctcggaa cctaccacga tctcctcaag atcattaagg ataaggattt cttggataac 2701 gaggaaaacg aggatatctt ggaggatatc gttcttaccc tcaccctctt tgaagataga 2761 gagatgattg aagaaaggct caagacctac gctcatctct tcgatgataa ggtgatgaag 2821 cagttgaaga gaagaagata cactggttgg ggaaggctct caagaaagct cattaacgga 2881 atcagggata agcagtctgg aaagacaatc cttgatttcc tcaagtctga tggattcgct 2941 aacagaaact tcatgcagct catccacgat gattctctca cctttaaaga ggatatccag 3001 aaggctcagg tttcaggaca gggtgatagt ctccatgagc atatcgctaa cctcgctgga 3061 tctcctgcaa tcaagaaggg aatcctccag actgtgaagg ttgtggatga gttggtgaag 3121 gtgatgggaa ggcataagcc tgagaacatc gtgatcgaaa tggctagaga gaaccagacc 3181 actcagaagg gacagaagaa ctctagggaa aggatgaaga ggatcgagga aggtatcaaa 3241 gagcttggat ctcagatcct caaagagcac cctgttgaga acactcagct ccagaatgag 3301 aagctctacc tctactacct ccagaacgga agggatatgt atgtggatca agagttggat 3361 atcaacaggc tctctgatta cgatgttgat catatcgtgc cacagtcatt cttgaaggat 3421 gattctatcg ataacaaggt gctcaccagg tctgataaga acaggggtaa gagtgataac 3481 gtgccaagtg aagaggttgt gaagaaaatg aagaactatt ggaggcagct cctcaacgct 3541 aagctcatca ctcagagaaa gttcgataac ttgactaagg ctgagagggg aggactctct 3601 gaattggata aggcaggatt catcaagagg cagcttgtgg aaaccaggca gatcactaag 3661 cacgttgcac agatcctcga ttctaggatg aacaccaagt acgatgagaa cgataagttg 3721 atcagggaag tgaaggttat caccctcaag tcaaagctcg tgtctgattt cagaaaggat 3781 ttccaattct acaaggtgag ggaaatcaac aactaccacc acgctcacga tgcttacctt 3841 aacgctgttg ttggaaccgc tctcatcaag aagtatccta agctcgagtc agagttcgtg 3901 tacggtgatt acaaggtgta cgatgtgagg aagatgatcg ctaagtctga gcaagagatc 3961 ggaaaggcta ccgctaagta tttcttctac tctaacatca tgaatttctt caagaccgag 4021 attaccctcg ctaacggtga gatcagaaag aggccactca tcgagacaaa cggtgaaaca 4081 ggtgagatcg tgtgggataa gggaagggat ttcgctaccg ttagaaaggt gctctctatg 4141 ccacaggtga acatcgttaa gaaaaccgag gtgcagaccg gtggattctc taaagagtct 4201 atcctcccta agaggaactc tgataagctc attgctagga agaaggattg ggaccctaag 4261 aaatacggtg gtttcgattc tcctaccgtg gcttactctg ttctcgttgt ggctaaggtt 4321 gagaagggaa agagtaagaa gctcaagtct gttaaggaac ttctcggaat cactatcatg 4381 gaaaggtcat ctttcgagaa gaacccaatc gatttcctcg aggctaaggg atacaaagag 4441 gttaagaagg atctcatcat caagctccca aagtactcac tcttcgaact cgagaacggt 4501 agaaagagga tgctcgcttc tgctggtgag cttcaaaagg gaaacgagct tgctctccca 4561 tctaagtacg ttaactttct ttacctcgct tctcactacg agaagttgaa gggatctcca 4621 gaagataacg agcagaagca acttttcgtt gagcagcaca agcactactt ggatgagatc 4681 atcgagcaga tctctgagtt ctctaaaagg gtgatcctcg ctgatgcaaa cctcgataag 4741 gtgttgtctg cttacaacaa gcacagagat aagcctatca gggaacaggc agagaacatc 4801 atccatctct tcacccttac caacctcggt gctcctgctg ctttcaagta cttcgataca 4861 accatcgata ggaagagata cacctctacc aaagaagtgc tcgatgctac cctcatccat 4921 cagtctatca ctggactcta cgagactagg atcgatctct cacagctcgg tggtgattca 4981 agggctgatc ctaagaagaa gaggaaggtt tgagcttgtc aagcagatcg ttcaaacatt 5041 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa 5101 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg 5161 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa 5221 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcga 5281 cgctactatt gtcttctgca cgaagtggtt taaactatca gtgtttgaca ggatatattg 5341 gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg 5401 aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt ccccagatca 5461 ggcgctggct gctgaacccc cagccggaac tgaccccaca aggccctagc gtttgcaatg 5521 caccaggtca tcattgaccc aggcgtgttc caccaggccg ctgcctcgca actcttcgca 5581 ggcttcgccg acctgctcgc gccacttctt cacgcgggtg gaatccgatc cgcacatgag 5641 gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat 5701 ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat atgaatttcg tgtagtggtc 5761 gccagcaaac agcacgacga tttcctcgtc gatcaggacc tggcaacggg acgttttctt 5821 gccacggtcc aggacgcgga agcggtgcag cagcgacacc gattccaggt gcccaacgcg 5881 gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac aggcattcct cggccttcgt 5941 gtaataccgg ccattgatcg accagcccag gtcctggcaa agctcgtaga acgtgaaggt 6001 gatcggctcg ccgatagggg tgcgcttcgc gtactccaac acctgctgcc acaccagttc 6061 gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg 6121 gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag 6181 ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc 6241 gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg 6301 cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt 6361 catagttcct cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct cctgttcaag 6421 acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg gcagggggag ccagttgcac 6481 gctgtcgcgc tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg actggaaggt 6541 ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa 6601 ccccgcgtcg atcagttctt gcctgtatgc cttccggtca aacgtccgat tcattcaccc 6661 tccttgcggg attgccccga ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc 6721 gcctggtgcc ttggtgtcca gataatccac cttatcggca atgaagtcgg tcccgtagac 6781 cgtctggccg tccttctcgt acttggtatt ccgaatcttg ccctgcacga ataccagcga 6841 ccccttgccc aaatacttgc cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa 6901 gaagtcggtg cgctcctgct tgtcgccggc atcgttgcgc cacatctagg atctgccagg 6961 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 7021 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 7081 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 7141 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 7201 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 7261 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 7321 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 7381 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 7441 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 7501 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 7561 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 7621 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 7681 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 7741 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 7801 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 7861 tctggcccca gtgctgcaat gataccgcga gaaccacgct caccggctcc agatttatca 7921 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 7981 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 8041 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 8101 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 8161 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 8221 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 8281 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 8341 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 8401 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 8461 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 8521 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 8581 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 8641 tatcaaggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 8701 ataggggttc cgcgcacgaa ttggccagcg ctgccatttt tggggtgagg ccgttcgcgg 8761 ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt agcgggccgg gagggttcga 8821 gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta 8881 aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa agacaggtta gcggtggccg 8941 aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc ctgtggacag cccctcaaat 9001 gtcaataggt gcgcccctca tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc 9061 cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc gcagggcact tatccccagg 9121 cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag 9181 gctggccagc tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt caacgccgcg 9241 ccgggtgagt cggcccctca agtgtcaacg tccgcccctc atctgtcagt gagggccaag 9301 ttttccgcga ggtatccaca acgccggcgg ccgcggtgtc tcgcacacgg cttcgacggc 9361 gtttctggcg cgtttgcagg gccatagacg gccgccagcc cagcggcgag ggcaaccagc 9421 ccggtgagcg tcgcaaagga gatcctgatc tgactgatgg gctgcctgta tcgagtggtg 9481 attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg 9541 gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact 9601 ggggtggatg cagtgggccc cac //