LOCUS Exported 11731 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of RET G751E. ACCESSION . VERSION . KEYWORDS pHAGE-RET-G751E SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11731) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 11731) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11731 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(6668..6692) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 6745..6786 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 6807..7374 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 6824..7376 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(6968..6985) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 7195..7214 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 7388..8107 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 7388..8104 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(7433..7454) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(7694..7713) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 8041..8062 /label=EGFP-C /note="EGFP, forward primer" misc_feature 8121..8709 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8174..8194) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8592..8603) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 8784..9017 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(9136..9154) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 9222..9326 /gene="bla" /label=AmpR promoter CDS 9327..10187 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9545..9564) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10358..10946 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10847..10866 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11100..11117 /label=L4440 /note="L4440 vector, forward primer" primer_bind 11209..11228 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 11357..11378 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 11393..11423 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11431..11447 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 11436..11458 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 11455..11471 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 11455..11471 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 11521..11540 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcaccatg gcgaaggcga cgtccggtgc cgcggggctg 3481 cgtctgctgt tgctgctgct gctgccgctg ctaggcaaag tggcattggg cctctacttc 3541 tcgagggatg cttactggga gaagctgtat gtggaccagg cggccggcac gcccttgctg 3601 tacgtccatg ccctgcggga cgcccctgag gaggtgccca gcttccgcct gggccagcat 3661 ctctacggca cgtaccgcac acggctgcat gagaacaact ggatctgcat ccaggaggac 3721 accggcctcc tctaccttaa ccggagcctg gaccatagct cctgggagaa gctcagtgtc 3781 cgcaaccgcg gctttcccct gctcaccgtc tacctcaagg tcttcctgtc acccacatcc 3841 cttcgtgagg gcgagtgcca gtggccaggc tgtgcccgcg tatacttctc cttcttcaac 3901 acctcctttc cagcctgcag ctccctcaag ccccgggagc tctgcttccc agagacaagg 3961 ccctccttcc gcattcggga gaaccgaccc ccaggcacct tccaccagtt ccgcctgctg 4021 cctgtgcagt tcttgtgccc caacatcagc gtggcctaca ggctcctgga gggtgagggt 4081 ctgcccttcc gctgcgcccc ggacagcctg gaggtgagca cgcgctgggc cctggaccgc 4141 gagcagcggg agaagtacga gctggtggcc gtgtgcaccg tgcacgccgg cgcgcgcgag 4201 gaggtggtga tggtgccctt cccggtgacc gtgtacgacg aggacgactc ggcgcccacc 4261 ttccccgcgg gcgtcgacac cgccagcgcc gtggtggagt tcaagcggaa ggaggacacc 4321 gtggtggcca cgctgcgtgt cttcgatgca gacgtggtac ctgcatcagg ggagctggtg 4381 aggcggtaca caagcacgct gctccccggg gacacctggg cccagcagac cttccgggtg 4441 gaacactggc ccaacgagac ctcggtccag gccaacggca gcttcgtgcg ggcgaccgta 4501 catgactata ggctggttct caaccggaac ctctccatct cggagaaccg caccatgcag 4561 ctggcggtgc tggtcaatga ctcagacttc cagggcccag gagcgggcgt cctcttgctc 4621 cacttcaacg tgtcggtgct gccggtcagc ctgcacctgc ccagtaccta ctccctctcc 4681 gtgagcagga gggctcgccg atttgcccag atcgggaaag tctgtgtgga aaactgccag 4741 gcattcagtg gcatcaacgt ccagtacaag ctgcattcct ctggtgccaa ctgcagcacg 4801 ctaggggtgg tcacctcagc cgaggacacc tcggggatcc tgtttgtgaa tgacaccaag 4861 gccctgcggc ggcccaagtg tgccgaactt cactacatgg tggtggccac cgaccagcag 4921 acctctaggc aggcccaggc ccagctgctt gtaacagtgg aggggtcata tgtggccgag 4981 gaggcgggct gccccctgtc ctgtgcagtc agcaagagac ggctggagtg tgaggagtgt 5041 ggcggcctgg gctccccaac aggcaggtgt gagtggaggc aaggagatgg caaagggatc 5101 accaggaact tctccacctg ctctcccagc accaagacct gccccgacgg ccactgcgat 5161 gttgtggaga cccaagacat caacatttgc cctcaggact gcctccgggg cagcattgtt 5221 gggggacacg agcctgggga gccccggggg attaaagctg gctatggcac ctgcaactgc 5281 ttccctgagg aggagaagtg cttctgcgag cccgaagaca tccaggatcc actgtgcgac 5341 gagctgtgcc gcacggtgat cgcagccgct gtcctcttct ccttcatcgt ctcggtgctg 5401 ctgtctgcct tctgcatcca ctgctaccac aagtttgccc acaagccacc catctcctca 5461 gctgagatga ccttccggag gcccgcccag gccttcccgg tcagctactc ctcttccagt 5521 gcccgccggc cctcgctgga ctccatggag aaccaggtct ccgtggatgc cttcaagatc 5581 ctggaggatc caaagtggga attccctcgg aagaacttgg ttcttggaaa aactctagga 5641 gaaggcgaat ttggaaaagt ggtcaaggca acggccttcc atctgaaagg cagagcagag 5701 tacaccacgg tggccgtgaa gatgctgaaa gagaacgcct ccccgagtga gcttcgagac 5761 ctgctgtcag agttcaacgt cctgaagcag gtcaaccacc cacatgtcat caaattgtat 5821 ggggcctgca gccaggatgg cccgctcctc ctcatcgtgg agtacgccaa atacggctcc 5881 ctgcggggct tcctccgcga gagccgcaaa gtggggcctg gctacctggg cagtggaggc 5941 agccgcaact ccagctccct ggaccacccg gatgagcggg ccctcaccat gggcgacctc 6001 atctcatttg cctggcagat ctcacagggg atgcagtatc tggccgagat gaagctcgtt 6061 catcgggact tggcagccag aaacatcctg gtagctgagg ggcggaagat gaagatttcg 6121 gatttcggct tgtcccgaga tgtttatgaa gaggattcgt acgtgaagag gagccagggt 6181 cggattccag ttaaatggat ggcaattgaa tccctttttg atcatatcta caccacgcaa 6241 agtgatgtat ggtcttttgg tgtcctgctg tgggagatcg tgaccctagg gggaaacccc 6301 tatcctggga ttcctcctga gcggctcttc aaccttctga agaccggcca ccggatggag 6361 aggccagaca actgcagcga ggagatgtac tgcctgatgc tgcaatgctg gaagcaggag 6421 ccggacaaaa ggccggtgtt tgcggacatc agcaaagacc tggagaagat gatggttaag 6481 aggagagact acttggacct tgcggcgtcc actccatctg actccctgat ttatgacgac 6541 ggcctctcag aggaggagac accgctggtg gactgtaata atgcccccct ccctcgagcc 6601 ctcccttcca catggattga aaacaaactc tatggtagaa tttcccatgc atttactaga 6661 ttctagaacc cagctttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctcg 6721 agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat 6781 tctacgcgta ccggttagta atgagatccc tccccccccc ctaacgttac tggccgaagc 6841 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct 6901 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt 6961 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct 7021 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc 7081 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag 7141 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc 7201 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga 7261 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt 7321 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc 7381 cacacatatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 7441 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 7501 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 7561 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca 7621 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 7681 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 7741 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 7801 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca 7861 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca 7921 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 7981 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca 8041 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 8101 caagtaaatc gatagatcct aatcaacctc tggattacaa aatttgtgaa agattgactg 8161 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt 8221 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc 8281 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt 8341 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga 8401 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct 8461 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat 8521 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct 8581 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc 8641 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg 8701 cctccccgcc tgagatcctt taagaccaat gacttacaag gcagctgtag atcttagcca 8761 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct 8821 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 8881 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 8941 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 9001 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc 9061 aaagaaatga atatcagaga gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt 9121 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa 9181 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 9241 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 9301 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 9361 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 9421 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 9481 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 9541 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc 9601 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 9661 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 9721 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 9781 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 9841 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat 9901 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 9961 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 10021 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 10081 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 10141 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 10201 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 10261 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 10321 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 10381 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 10441 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 10501 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 10561 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 10621 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 10681 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 10741 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 10801 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 10861 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 10921 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 10981 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 11041 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 11101 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc 11161 gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga 11221 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 11281 cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca 11341 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact 11401 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa 11461 acagctatga catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt 11521 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag 11581 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt 11641 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta 11701 tttgttaaat atgtactaca aacttagtag t //