LOCUS Exported 831 bp ds-DNA linear SYN 13-JUN-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS TLD_062 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 831) AUTHORS Deans TL, Cantor CR, Collins JJ TITLE A tunable genetic switch based on RNAi and repressor proteins for regulating gene expression in mammalian cells. JOURNAL Cell. 2007 Jul 27. 130(2):363-72. PUBMED 17662949 REFERENCE 2 (bases 1 to 831) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..831 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 116..136 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 287..306 /label=LKO.1 5' /note="Human U6 promoter, forward primer" protein_bind 318..336 /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O1 for the tetR and tetA genes" primer_bind complement(430..449) /label=T7 /note="T7 promoter, forward primer" promoter complement(431..449) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(456..473) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(456..472) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(465..487) /label=M13/pUC Forward /note="In lacZ gene" primer_bind complement(700..719) /label=F1ori-R /note="F1 origin, reverse primer" ORIGIN 1 agctctccca tatggtcgac ctgcaggcgg ccgcgaattc actagtgatt aggcaaaacg 61 caccacgtga cggagcgtga ccgcgcgccg agcgcgcgcc aaggtcgggc aggaagaggg 121 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat 181 tagaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta 241 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct 301 taccgtaact tgaaagtact ctatcattga tagagttata tatcttgtgg aaaggacgaa 361 acaccgctac acaaatcagc gatttttcaa gagaaaatcg ctgatttgtg tagaaaaagg 421 cccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt 481 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc 541 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg 601 aatggcgaan ggacgcgccc tgtagcggcg cantaagcgc ggcgggtgtg gtggttacgc 661 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 721 cctttctcgc cacgttcgcc ggctttcccc gtcanctcta aatcgggggc tccctttagg 781 gttccnattt agtgctttnn ggcncctcga ccccaaaaan ttgattnagg g //