LOCUS Exported 5080 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION –2.3kb promoter of zebrafish fabp2/ifabp gene in Gateway 5' Entry vector. ACCESSION . VERSION . KEYWORDS p5E-2.3k-fabp2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5080) TITLE –2.3kb promoter of zebrafish fabp2/ifabp gene in Gateway 5' Entry vector JOURNAL Unpublished REFERENCE 2 (bases 1 to 5080) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5080 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 1..589 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 490..509 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 743..760 /label=L4440 /note="L4440 vector, forward primer" terminator 919..946 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 1038..1124 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 1173..1195 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 1187..1204 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 1188..1204 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 1244..1339 /gene="mutant version of attL" /label=attL4 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 1341..1359 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind 1384..1400 /label=pBluescriptKS /note="For pBluescript vector" primer_bind 1385..1401 /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3705..3721) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3705..3721) /label=pBluescriptSK /note="For pBluescript vector" promoter complement(3758..3776) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" protein_bind 3776..3900 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter complement(3973..3991) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3996..4012) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3996..4012) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4114..4133) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 4125..4934 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(4192..4211) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" ORIGIN 1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat 781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga 901 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc 961 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc 1021 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata 1081 aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc 1141 tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc 1201 cagtcttaag ctcgggcccg agttaacgct accatggagc tccaaataat gattttattt 1261 tgactgatag tgacctgttc gttgcaacaa attgataagc aatgcttttt tataatgcca 1321 actttgtata gaaaagttgg taatacgact cactataggg cgaattgggt accgggcccc 1381 ccctcgaggt cgacggtatc gataagcttc ccttccagct gcaacccatc actgggaaac 1441 actcatacac tcacagtcac acagtttagc ctacgcaatt cacctatata ttcatcacat 1501 atctttggac ttgtggggga aaccggaaac tccacacaga aatgccaact gacccagctg 1561 aggttcaaac cagcccttct tactgtgagg tgacagcgct agccattgcg ccaccgctcc 1621 accctttatt ttaatttatt taattgaatt tattttattg tatttgtcct aaattgccag 1681 tacaatcagt acaatcatta gaatggtttc tgaaggttca tgcctgtaat aactgcttta 1741 tatttaaatt tgtctatagc aataagattt agtaataata ttaataattt tatattctat 1801 tctattatta tattatatat acattttcac actaaaccga aaagagactt cttattaacg 1861 acctcaaaaa aataactaaa tctgaaaaag atcactgtta cgcagattct gcaaattcta 1921 caaaatgttt gtagtaaata tagtataaaa gatgttttta gcttactttt tacattattt 1981 tatacattta aacatgcatg aaatcaatta ttatcagctt ggaccttttc ttgtccctac 2041 tcttagtggg acgatgccaa aggtcttaca atgggacgat accaaaggtc ttcagaggtc 2101 ttatttaatt gcactgttaa gagattaagg ataaggtaat gatgagataa accaccatca 2161 gagattatca tgtctcagtt tgatatctcg tctctttatc ttgtcatgga gcctccaggt 2221 aaaacttcca cactgcatgt tgcttaattt gaatcatttg caggctttgt acactattca 2281 tcattacaga taatgctgaa gctgttcttt cacagccttg catcaatatt tacaacattt 2341 tcaaacaata caaggggata attcagtaat acaatttaaa ataaggttcc aataactata 2401 ttactaataa ctaacaatga gcaaaataca taatgctgtt attttagtat aactggatga 2461 gtactgcaga ttttaaataa aatattcatg gaattactgt cgttactgta taatgttggt 2521 gaatgtatgg tctgtttttg acatttgtgt gcatttattt ttatatctaa agtgttttat 2581 agatttgcat tttaatatta gtagtaaatg caaatgataa atactttatc tttaaattaa 2641 aaatattatt atgcattact tatctgtgat aaatatatcg tatgtgttat gttactgata 2701 aaatgttaca ataataatgg ttcggttagg gttaaggttt taggacaaaa cacaactgtt 2761 cattagtatt cagatcagat ctatgaaata atattggcag gtgcaaattt tcattttaat 2821 aatgtactag tactgtaaat atttgagtta gctttagaac tggctttgaa gtatattcac 2881 tggtagctgt ctgttaagta gaactgcaca aaaaaaaggt acgaggaaga acgaagcatt 2941 gccatatact gtattctcaa ttttagctgc aatagctgca cttttgtagt aaaaagttct 3001 tcaaagaccc tttttatata gtgtgaagaa cttttcaata atcttaaaac atttttctac 3061 ataaataaaa tctttgtgca atgaaatatt tcaaaaagta ctttataaaa ccaaccattc 3121 atgtcaacac agagccttaa aataattgtt accagtattt ctcaatagcc aatgaggcat 3181 gcagcaagat tgtaaactaa tttaactgta accattaaat ctaaagtttt gacctttgac 3241 ctttcaccac gattgtttaa aaatcaatac atgtggctag aattcatagc tttcaaacac 3301 tcttcatagt ccctcagtag ttactgcaac acaaggcact tactagtata acagacacac 3361 cctacacagt ttaaacagcg aacaattcaa acaaaagcag gactttgtta tgagaacaac 3421 agagcgtgta tgtttgacca tcgcagtaca agtgagtgct tgttctcagg acttgaaatc 3481 tccgctcttt gcacaatcaa tgaataagca aggcatgctg ggatgtgtgt aacatatagc 3541 ctgttgggcg ggtgagattt atacttggtg agctttactc ggccacatca gcatgaagat 3601 aatactcaga taaggcaacg cttcgccact cgcacaggta taaaagagtg gctcggggta 3661 aagttaggcc actgtcagga tcacacaaca gtctgtcatc atcggatcca ctagttctag 3721 agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattcaag 3781 tttgtacaaa aaagttgaac gagaaacgta aaatgatata aatatcaata tattaaatta 3841 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatgc agtcactatg 3901 aaccaactac ttagatggta ttagtgacct gtagaattcg agctctagag ctgcagggcg 3961 gccgcgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct 4021 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa 4081 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac 4141 gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat 4201 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg 4261 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg 4321 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta 4381 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc 4441 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc 4501 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc 4561 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg 4621 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat 4681 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg 4741 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg 4801 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt 4861 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg 4921 atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga 4981 cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc 5041 actgagcgtc agaccccgta gaaaagatca aaggatcttc //