LOCUS Exported 9006 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pAG426GPD-EGFP-ccdB SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9006) TITLE S cerevisiae Advanced Gateway Destination Vectors REFERENCE 2 (bases 1 to 9006) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9006 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(10..28) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" rep_origin 69..1411 /label=2u ori /note="yeast 2u plasmid origin of replication" protein_bind 401..448 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." promoter 1438..1542 /gene="bla" /label=AmpR promoter CDS 1543..2403 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1761..1780) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2574..3162 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3063..3082 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3316..3333 /label=L4440 /note="L4440 vector, forward primer" protein_bind 3450..3471 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 3486..3516 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3524..3540 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 3529..3551 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 3548..3564 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 3548..3564 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 3583..3603 /label=T3 /note="T3 promoter, forward primer" promoter 3585..3603 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 3628..4271 /gene="S. cerevisiae TDH3" /label=GAP promoter /note="promoter for glyceraldehyde-3-phosphate dehydrogenase" primer_bind 4165..4188 /label=GPDpro-F /note="S. cerevisiae GPD promoter, forward primer" primer_bind 4277..4293 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind 4277..4293 /label=pBluescriptSK /note="For pBluescript vector" CDS 4306..5022 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 4306..5022 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(4351..4372) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(4612..4631) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 4959..4980 /label=EGFP-C /note="EGFP, forward primer" protein_bind 5047..5171 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 5196..5226 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 5280..5960 /codon_start=1 /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE DPAY" primer_bind complement(5346..5365) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 6280..6585 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 6464..6483 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(6626..6750) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" terminator 6757..7004 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" primer_bind complement(6773..6791) /label=CYC1 /note="CYC1 transcription termination signal, reverse primer" primer_bind complement(7022..7041) /label=T7 /note="T7 promoter, forward primer" promoter complement(7023..7041) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(7051..7068) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(7051..7067) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7060..7082) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 7208..7663 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7295..7314) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7505..7526 /label=F1ori-F /note="F1 origin, forward primer" CDS complement(7794..8597) /codon_start=1 /gene="S. cerevisiae URA3" /product="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" /label=URA3 /note="yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN" promoter complement(8598..8813) /gene="S. cerevisiae URA3" /label=URA3 promoter primer_bind complement(8837..8856) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 8956..8978 /label=pGEX 3' /note="pGEX vectors, reverse primer" ORIGIN 1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 61 cttagtatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg 121 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg 181 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac 241 gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata 301 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc 361 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt 421 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa 481 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga 541 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat 601 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct 661 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta 721 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag 781 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc 841 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct 901 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt 961 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg 1021 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa 1081 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa 1141 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta 1201 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt 1261 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt 1321 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt 1381 gttctacaaa atgaagcaca gatgcttcgt tcaggtggca cttttcgggg aaatgtgcgc 1441 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 1501 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 1561 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 1621 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 1681 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 1741 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 1801 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 1861 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 1921 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 1981 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 2041 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 2101 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 2161 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 2221 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 2281 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 2341 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 2401 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 2461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 2521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 2581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 2641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 2701 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac 2761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 2821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 2881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 2941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 3001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 3061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 3121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 3181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 3241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 3301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa 3361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 3421 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc 3481 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa 3541 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa 3601 agggaacaaa agctggagct cagtttatca ttatcaatac tcgccatttc aaagaatacg 3661 taaataatta atagtagtga ttttcctaac tttatttagt caaaaaatta gccttttaat 3721 tctgctgtaa cccgtacatg cccaaaatag ggggcgggtt acacagaata tataacatcg 3781 taggtgtctg ggtgaacagt ttattcctgg catccactaa atataatgga gcccgctttt 3841 taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 3901 catcagttca taggtccatt ctcttagcgc aactacagag aacaggggca caaacaggca 3961 aaaaacgggc acaacctcaa tggagtgatg caacctgcct ggagtaaatg atgacacaag 4021 gcaattgacc cacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 4081 ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 4141 acttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 4201 ttaaacttct taaattctac ttttatagtt agtctttttt ttagttttaa aacaccagaa 4261 cttagtttcg acggattcta gaactagtgg atcccccggg acaaaatggt gagcaagggc 4321 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc 4381 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg 4441 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg 4501 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc 4561 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc 4621 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag 4681 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac 4741 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac 4801 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag 4861 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag 4921 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg 4981 accgccgccg ggatcactct cggcatggac gagctgtaca agggtggacc aggtggtgga 5041 catcaaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat 5101 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc 5161 cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt 5221 ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa 5281 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac 5341 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata 5401 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc 5461 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg 5521 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa 5581 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt 5641 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga 5701 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg 5761 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg 5821 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg 5881 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcaggcg gggcgtaatc 5941 tagaggatcc ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg 6001 cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag gtatgctatg 6061 aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg 6121 atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg 6181 ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa 6241 tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc 6301 tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg 6361 cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtcccc 6421 cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat 6481 atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa 6541 aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccctt 6601 atacacagcc agtctgcagg tcgaccatag tgactggata tgttgtgttt tacagtatta 6661 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt 6721 ttctcgttca gctttcttgt acaaagtggt tcgatgtcat gtaattagtt atgtcacgct 6781 tacattcacg ccctcccccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg 6841 aagtctaggt ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat 6901 ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa 6961 ccttgcttga gaaggttttg ggacgctcga aggctttaat ttgcggccgg tacccaattc 7021 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg 7081 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg 7141 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg 7201 cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc 7261 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt 7321 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc 7381 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt 7441 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt 7501 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt 7561 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa 7621 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttcctgatg cggtattttc 7681 tccttacgca tctgtgcggt atttcacacc gcatagggta ataactgata taattaaatt 7741 gaagctctaa tttgtgagtt tagtatacat gcatttactt ataatacagt tttttagttt 7801 tgctggccgc atcttctcaa atatgcttcc cagcctgctt ttctgtaacg ttcaccctct 7861 accttagcat cccttccctt tgcaaatagt cctcttccaa caataataat gtcagatcct 7921 gtagagacca catcatccac ggttctatac tgttgaccca atgcgtctcc cttgtcatct 7981 aaacccacac cgggtgtcat aatcaaccaa tcgtaacctt catctcttcc acccatgtct 8041 ctttgagcaa taaagccgat aacaaaatct ttgtcgctct tcgcaatgtc aacagtaccc 8101 ttagtatatt ctccagtaga tagggagccc ttgcatgaca attctgctaa catcaaaagg 8161 cctctaggtt cctttgttac ttcttctgcc gcctgcttca aaccgctaac aatacctggg 8221 cccaccacac cgtgtgcatt cgtaatgtct gcccattctg ctattctgta tacacccgca 8281 gagtactgca atttgactgt attaccaatg tcagcaaatt ttctgtcttc gaagagtaaa 8341 aaattgtact tggcggataa tgcctttagc ggcttaactg tgccctccat ggaaaaatca 8401 gtcaagatat ccacatgtgt ttttagtaaa caaattttgg gacctaatgc ttcaactaac 8461 tccagtaatt ccttggtggt acgaacatcc aatgaagcac acaagtttgt ttgcttttcg 8521 tgcatgatat taaatagctt ggcagcaaca ggactaggat gagtagcagc acgttcctta 8581 tatgtagctt tcgacatgat ttatcttcgt ttcctgcagg tttttgttct gtgcagttgg 8641 gttaagaata ctgggcaatt tcatgtttct tcaacactac atatgcgtat atataccaat 8701 ctaagtctgt gctccttcct tcgttcttcc ttctgttcgg agattaccga atcaaaaaaa 8761 tttcaaggaa accgaaatca aaaaaaagaa taaaaaaaaa atgatgaatt gaaaaggtgg 8821 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc 8881 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac 8941 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac 9001 gcgcga //