LOCUS Exported 9235 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pAG425GAL-ccdB SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9235) TITLE S cerevisiae Advanced Gateway Destination Vectors REFERENCE 2 (bases 1 to 9235) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9235 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(10..28) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" rep_origin 69..1411 /label=2u ori /note="yeast 2u plasmid origin of replication" protein_bind 401..448 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." promoter 1438..1542 /gene="bla" /label=AmpR promoter CDS 1543..2403 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1761..1780) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2574..3162 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3063..3082 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3316..3333 /label=L4440 /note="L4440 vector, forward primer" protein_bind 3450..3471 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 3486..3516 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3524..3540 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 3529..3551 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 3548..3564 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 3548..3564 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 3583..3603 /label=T3 /note="T3 promoter, forward primer" promoter 3585..3603 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 3627..4068 /gene="S. cerevisiae GAL1" /label=GAL1 promoter /note="inducible promoter, regulated by Gal4" protein_bind 3627..3744 /label=UAS /bound_moiety="Gal4" /note="upstream activating sequence mediating Gal4-dependent induction" primer_bind 4039..4062 /label=GAL1 /note="S. cerevisiae GAL1 promoter, forward primer" primer_bind 4083..4099 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind 4083..4099 /label=pBluescriptSK /note="For pBluescript vector" protein_bind 4107..4231 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 4256..4286 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 4340..4999 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(4406..4425) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 5341..5646 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 5525..5544 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(5687..5811) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(5844..5860) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5845..5861) /label=pBluescriptKS /note="For pBluescript vector" terminator 5863..6110 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" primer_bind complement(5879..5897) /label=CYC1 /note="CYC1 transcription termination signal, reverse primer" primer_bind complement(6128..6147) /label=T7 /note="T7 promoter, forward primer" promoter complement(6129..6147) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(6157..6174) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(6157..6173) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6166..6188) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 6314..6769 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6401..6420) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6611..6632 /label=F1ori-F /note="F1 origin, forward primer" promoter 7070..7477 /gene="S. cerevisiae LEU2" /label=LEU2 promoter CDS 7478..8572 /codon_start=1 /gene="S. cerevisiae LEU2" /product="3-isopropylmalate dehydrogenase, required for leucine biosynthesis" /label=LEU2 /note="yeast auxotrophic marker" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG DLGGSNSTTEVGDAVAEEVKKILA" primer_bind complement(9066..9085) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 9185..9207 /label=pGEX 3' /note="pGEX vectors, reverse primer" ORIGIN 1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 61 cttagtatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg 121 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg 181 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac 241 gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata 301 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc 361 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt 421 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa 481 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga 541 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat 601 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct 661 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta 721 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag 781 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc 841 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct 901 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt 961 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg 1021 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa 1081 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa 1141 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta 1201 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt 1261 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt 1321 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt 1381 gttctacaaa atgaagcaca gatgcttcgt tcaggtggca cttttcgggg aaatgtgcgc 1441 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 1501 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 1561 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 1621 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 1681 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 1741 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 1801 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 1861 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 1921 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 1981 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 2041 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 2101 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 2161 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 2221 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 2281 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 2341 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 2401 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 2461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 2521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 2581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 2641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 2701 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac 2761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 2821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 2881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 2941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 3001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 3061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 3121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 3181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 3241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 3301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa 3361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 3421 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc 3481 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa 3541 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa 3601 agggaacaaa agctggagct ctagtacgga ttagaagccg ccgagcgggt gacagccctc 3661 cgaaggaaga ctctcctccg tgcgtcctcg tcttcaccgg tcgcgttcct gaaacgcaga 3721 tgtgcctcgc gccgcactgc tccgaacaat aaagattcta caatactagc ttttatggtt 3781 atgaagagga aaaattggca gtaacctggc cccacaaacc ttcaaatgaa cgaatcaaat 3841 taacaaccat aggatgataa tgcgattagt tttttagcct tatttctggg gtaattaatc 3901 agcgaagcga tgatttttga tctattaaca gatatataaa tgcaaaaact gcataaccac 3961 tttaactaat actttcaaca ttttcggttt gtattacttc ttattcaaat gtaataaaag 4021 tatcaacaaa aaattgttaa tatacctcta tactttaacg tcaaggagaa aaaaccccgg 4081 attctagaac tagtggatcc cccatcacaa gtttgtacaa aaaagctgaa cgagaaacgt 4141 aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag actacataat 4201 actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc caggctttac 4261 actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc 4321 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc 4381 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa 4441 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa 4501 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg 4561 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt 4621 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg 4681 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt 4741 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac 4801 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg 4861 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc 4921 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga 4981 gtggcagggc ggggcgtaaa cgcgtggatc cggcttacta aaagccagat aacagtatgc 5041 gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat 5101 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat 5161 cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga 5221 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg 5281 aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg ggctggtgaa 5341 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta 5401 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt 5461 ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc 5521 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg 5581 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga 5641 atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat 5701 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt 5761 gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg tgatgggctg 5821 caggaattcg atatcaagct tatcgatacc gtcgacctcg agtcatgtaa ttagttatgt 5881 cacgcttaca ttcacgccct ccccccacat ccgctctaac cgaaaaggaa ggagttagac 5941 aacctgaagt ctaggtccct atttattttt ttatagttat gttagtatta agaacgttat 6001 ttatatttca aatttttctt ttttttctgt acagacgcgt gtacgcatgt aacattatac 6061 tgaaaacctt gcttgagaag gttttgggac gctcgaaggc tttaatttgc ggccggtacc 6121 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg 6181 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc 6241 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct 6301 gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 6361 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 6421 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta 6481 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 6541 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg 6601 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat 6661 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt 6721 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc ctgatgcggt 6781 attttctcct tacgcatctg tgcggtattt cacaccgcat atcgaccggt cgaggagaac 6841 ttctagtata tctacatacc taatattatt gccttattaa aaatggaatc ccaacaatta 6901 catcaaaatc cacattctct tcaaaatcaa ttgtcctgta cttccttgtt catgtgtgtt 6961 caaaaacgtt atatttatag gataattata ctctatttct caacaagtaa ttggttgttt 7021 ggccgagcgg tctaaggcgc ctgattcaag aaatatcttg accgcagtta actgtgggaa 7081 tactcaggta tcgtaagatg caagagttcg aatctcttag caaccattat ttttttcctc 7141 aacataacga gaacacacag gggcgctatc gcacagaatc aaattcgatg actggaaatt 7201 ttttgttaat ttcagaggtc gcctgacgca tatacctttt tcaactgaaa aattgggaga 7261 aaaaggaaag gtgagagcgc cggaaccggc ttttcatata gaatagagaa gcgttcatga 7321 ctaaatgctt gcatcacaat acttgaagtt gacaatatta tttaaggacc tattgttttt 7381 tccaataggt ggttagcaat cgtcttactt tctaactttt cttacctttt acatttcagc 7441 aatatatata tatatatttc aaggatatac cattctaatg tctgccccta agaagatcgt 7501 cgttttgcca ggtgaccacg ttggtcaaga aatcacagcc gaagccatta aggttcttaa 7561 agctatttct gatgttcgtt ccaatgtcaa gttcgatttc gaaaatcatt taattggtgg 7621 tgctgctatc gatgctacag gtgttccact tccagatgag gcgctggaag cctccaagaa 7681 ggctgatgcc gttttgttag gtgctgtggg tggtcctaaa tggggtaccg gtagtgttag 7741 acctgaacaa ggtttactaa aaatccgtaa agaacttcaa ttgtacgcca acttaagacc 7801 atgtaacttt gcatccgact ctcttttaga cttatctcca atcaagccac aatttgctaa 7861 aggtactgac ttcgttgttg tcagagaatt agtgggaggt atttactttg gtaagagaaa 7921 ggaagacgat ggtgatggtg tcgcttggga tagtgaacaa tacaccgttc cagaagtgca 7981 aagaatcaca agaatggccg ctttcatggc cctacaacat gagccaccat tgcctatttg 8041 gtccttggat aaagctaatg ttttggcctc ttcaagatta tggagaaaaa ctgtggagga 8101 aaccatcaag aacgaattcc ctacattgaa ggttcaacat caattgattg attctgccgc 8161 catgatccta gttaagaacc caacccacct aaatggtatt ataatcacca gcaacatgtt 8221 tggtgatatc atctccgatg aagcctccgt tatcccaggt tccttgggtt tgttgccatc 8281 tgcgtccttg gcctctttgc cagacaagaa caccgcattt ggtttgtacg aaccatgcca 8341 cggttctgct ccagatttgc caaagaataa ggtcaaccct atcgccacta tcttgtctgc 8401 tgcaatgatg ttgaaattgt cattgaactt gcctgaagaa ggtaaggcca ttgaagatgc 8461 agttaaaaag gttttggatg caggtatcag aactggtgat ttaggtggtt ccaacagtac 8521 caccgaagtc ggtgatgctg tcgccgaaga agttaagaaa atccttgctt aaaaagattc 8581 tcttttttta tgatatttgt acataaactt tataaatgaa attcataata gaaacgacac 8641 gaaattacaa aatggaatat gttcataggg tagacgaaac tatatacgca atctacatac 8701 atttatcaag aaggagaaaa aggaggatgt aaaggaatac aggtaagcaa attgatacta 8761 atggctcaac gtgataagga aaaagaattg cactttaaca ttaatattga caaggaggag 8821 ggcaccacac aaaaagttag gtgtaacaga aaatcatgaa actatgattc ctaatttata 8881 tattggagga ttttctctaa aaaaaaaaaa atacaacaaa taaaaaacac tcaatgacct 8941 gaccatttga tggagtttaa gtcaatacct tcttgaacca tttcccataa tggtgaaagt 9001 tccctcaaga attttactct gtcagaaacg gccttaacga cgtagtcgat atggtgcact 9061 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc 9121 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc 9181 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcga //