LOCUS       Exported               12341 bp ds-DNA     circular SYN 22-JAN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCL-Eco
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12341)
  AUTHORS   Naviaux RK, Costanzi E, Haas M, Verma IM
  TITLE     The pCL vector system: rapid production of helper-free, high-titer, 
            recombinant retroviruses.
  JOURNAL   J Virol. 1996 Aug . 70(8):5701-5.
  PUBMED    8764092
REFERENCE   2  (bases 1 to 12341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 22, 2024 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12341
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1..861
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(219..238)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1032..1620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1521..1540
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1774..1791
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      1856..1990
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1909..1928)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    2448..2582
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2472..2491)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2526..2545
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2854..2874)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          3004..3069
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(5719..10935)
                     /codon_start=1
                     /gene="gag-pol"
                     /product="Moloney murine leukemia virus Gag-Pol 
                     polyprotein"
                     /label=gag-pol
                     /note="contains a read-through stop codon"
                     /translation="MGQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPT
                     FNVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPK
                     PPPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGAKPKPQVLSDSGGPLIDLLTEDPP
                     PYRDPRPPPSDRDGNGGEATPAGEAPDPSPMASRLRGRREPPVADSTTSQAFPLRAGGN
                     GQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLITHQPTWDDCQQLLGTLLTGE
                     EKQRVLLEARKAVRGDDGRPTQLPNEVDAAFPLERPDWDYTTQAGRNHLVHYRQLLLAG
                     LQNAGRSPTNLAKVKGITQGPNESPSAFLERLKEAYRRYTPYDPEDPGQETNVSMSFIW
                     QSAPDIGRKLERLEDLKNKTLGDLVREAEKIFNKRETPEEREERIRRETEEKEERRRTE
                     DEQKEKERDRRRHREMSKLLATVVSGQKQDRQGGERRRSQLDRDQCAYCKEKGHWAKDC
                     PKKPRGPRGPRPQTSLLTLDD*GGQGQEPPPEPRITLKVGGQPVTFLVDTGAQHSVLTQ
                     NPGPLSDKSAWVQGATGGKRYRWTTDRKVHLATGKVTHSFLHVPDCPYPLLGRDLLTKL
                     KAQIHFEGSGAQVMGPMGQPLQVLTLNIEDEYRLHETSKEPDVSLGSTWLSDFPQAWAE
                     TGGMGLAVRQAPLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPW
                     NTPLLPVKKPGTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKD
                     AFFCLRLHPTSQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFDEALHRDLADFRI
                     QHPDLILLQYVDDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGY
                     LLKEGQRWLTEARKETVMGQPTPKTPRQLREFLGTAGFCRLWIPGFAEMAAPLYPLTKT
                     GTLFNWGPDQQKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWR
                     RPVAYLSKKLDPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPP
                     DRWLSNARMTHYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDILAEAHGTRP
                     DLTDQPLPDADHTWYTDGSSLLQEGQRKAGAAVTTETEVIWAKALPAGTSAQRAELIAL
                     TQALKMAEGKKLNVYTDSRYAFATAHIHGEIYRRRGLLTSEGKEIKNKDEILALLKALF
                     LPKRLSIIHCPGHQKGHSAEARGNRMADQAARKAAITETPDTSTLLIENSSPYTSEHFH
                     YTVTDIKDLTKLGAIYDKTKKYWVYQGKPVMPDQFTFELLDFLHQLTHLSFSKMKALLE
                     RSHSPYYMLNRDRTLKNITETCKACAQVNASKSAVKQGTRVRGHRPGTHWEIDFTEIKP
                     GLYGYKYLLVFIDTFSGWIEAFPTKKETAKVVTKKLLEEIFPRFGMPQVLGTDNGPAFV
                     SKVSQTVADLLGIDWKLHCAYRPQSSGQVERMNRTIKETLTKLTLATGSRDWVLLLPLA
                     LYRARNTPGPHGLTPYEILYGAPPPLVNFPDPDMTRVTNSPSLQAHLQALYLVQHEVWR
                     PLAAAYQEQLDRPVVPHPYRVGDTVWVRRHQTKNLEPRWKGPYTVLLTTPTALKVDGIA
                     AWIHAAHVKAADPGGGPSSRLTWRVQRSQNPLKIRLTREAP"
     misc_feature    5782..6156
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(5828..5844)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     primer_bind     complement(10532..10548)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(10564..10586)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     promoter        complement(11244..11447)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(11273..11293)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        11448..11827
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(11972..11991)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12151..12169)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12237..12341
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
       61 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      121 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      181 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      241 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      301 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      361 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
      421 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
      481 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
      541 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
      601 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
      661 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
      721 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
      781 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
      841 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
      901 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
      961 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1021 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1081 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1141 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     1201 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1261 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1321 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1381 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     1441 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     1501 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     1561 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     1621 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     1681 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     1741 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     1801 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcctc
     1861 caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg
     1921 cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta
     1981 ggggcgggat gggcggagta agacccgcac ccgttcccaa actgcttatt aaagggggta
     2041 tggaggtgct ggaccttgtg acagggccag acagtgtgac agaaatagaa gcagtcctgt
     2101 tttgacaagt tgcctctgga agcctctaca atgcctctct tctttttctc cagagtaagc
     2161 ggaggccagg ggcccccggc ctctgcttaa tactaaaaaa aacagctgtt gtcatagtaa
     2221 tgattgggtg gaaacattct aggcctgggt ggagaggctt tttgcttcct cttgcaaaac
     2281 cacactgccc tctggagggc agttgcctag caactaatta aaagaggatg tcgcacggcc
     2341 agctgcggtc agttagtcac ttcctgctta actgacttga cattttctat tttaagagtc
     2401 gggaggaaaa ttactgtgtt ggaggccctc cgccatcttc tgaagctgat ccagacatga
     2461 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     2521 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     2581 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     2641 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatctc tagtcaaggc
     2701 actatacatc aaatattttt ccataatttt cttgtatagc agtgcagctt tttcctttgt
     2761 ggtgtaaata gcaaagcaag caagagttct attactaaac acagcatgac tcaaaaaact
     2821 tagcaattct gaaggaaagt ccttggggtc ttctaccttt ctcttctttt ttggaggagt
     2881 agaatgttga gagtcagcag tagcctcatc atcactagat ggcatttctt ctgagcaaaa
     2941 caggttttcc tcattaaagg cattccacca ctgctcccat tcatcagttc cataggttgg
     3001 aatctaaaat acacaaacaa ttagaatcag tagtttaaca cattatacac ttaaaaattt
     3061 tatatttacc ttagagcttt aaatctctgt aggtagtttg tccaattatg tcacaccaca
     3121 gaagtaaggt tccttcacaa agatcctcta gctacctagc tagatacact ccgctatcgc
     3181 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     3241 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     3301 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagccagct tacctcccgg
     3361 tggtgggtcg gtggtccctg ggcaggggtc tcccgatccc ggacgagccc ccaaatgaaa
     3421 gacccccgct gacgggtagt caatcactca gaggagaccc tcccaaggaa cagcgagacc
     3481 acaagtcgga tgcaactgca agagggttta ttggatacac gggtacccgg gcgactcagt
     3541 caatcggagg actggcgccc cgagtgaggg gttgtgggct cttttattga gctcggggag
     3601 cagaagcgcg cgaacagaag cgagaagcga actgattggt tagttcaaat aaggcacagg
     3661 gtcatttcag gtccttgggg caccctggaa acatctgatg gttctctagc ttgccaaacc
     3721 tacaggtggg gtctttcatt cccccctttt tctggagact aaataaaatc ttttatttta
     3781 tctatggctc gtactctata ggcttcagct ggtgatattg ttgagtcaaa actagagcct
     3841 ggaccactga tatcctgtct ttaacaaatt ggactaatcg attaagaatg cagggtccga
     3901 agagcaaaat cattaggagt acaatgaggg gtcccataat ggtagatatc aaggtggtaa
     3961 accaagggga tctgttaaac agtccctcaa accatccttg agttgactca aacagtttct
     4021 gtctctgatt aagcctctct ctcaatttgg ccatgctgtc tctcactagt cctgtgtggt
     4081 ccgcatagaa gcaacattct tcttttagag cagcacacag ccctccttct tttagaaata
     4141 acaagtctag gccccttcga ttctgtagga caacttcaga cagggaagtg agagactttt
     4201 ctaggttaga gattgatttt tcaacctccc tgagatcatc ctgtactgcg gcttggagct
     4261 gctggaattg ctgagtggcc attagagcag tagtccctgt tcctattcca gcggcaattc
     4321 cccccatggt tagtccaccc aataataggg ccagggttaa cgacaccggt tctcttttgt
     4381 gtcggttgga tctctcaaac aggccgtaaa catagctggg ggaatgatag gtgactcttg
     4441 gccagagttc gacaagaaca caataatcag tggtaaggtt cagtatggtg gtggagatgc
     4501 atggagtaag cccggtacta caagcccaca tggtacctgt aggggcaact agataatagg
     4561 accctcgact gcttgtctgg gtggtattac atagggcctg atgtgttttg ggaactgctc
     4621 ctatgcagag tccctgtccg gtcacttcgg acagggtcaa cttgtgttgg gaggccacgg
     4681 agcagttggc tggagcagag gtatggttgg agtaggtacc caggacggca accccttcgt
     4741 agtagggggg tcccgctact agacacaacc agcactcttg ggttttgtca ggactggtga
     4801 ggttgagggc ttggtaggct ccgtctacta agtttagcag cctattttcc gttcccgccg
     4861 gtggaagttg ggtaggggag agaggagtcc cactgggtgg tttggtgact gaaggcgact
     4921 taacaggttt gggcttggag agtggctgtt ggtctgccag aacggggttt ggccctattg
     4981 ggacgcgggg tcctagattt tggtatctga gtcggatccc aaatgtaagc cctggatctt
     5041 gtccggagac atacaaacgt aagccccagt aatgtcctgt ggtccaggaa gtaacccgtc
     5101 tcccggcgtc tgtaaaccga ataactaagg ggttgcacca cttattatct ttgcatacct
     5161 ggacagcctg gtcagaggtg agattgttgt ttactgtgat gaaatcccat gatgaggagg
     5221 gcttccagta agctctaccg gttgtctcac agccccaata ggcacagtag aaggagtctg
     5281 gacccccaca tgacttggat tctcgggggc ggtggggccc ggggcaaaca taaaatccct
     5341 catttgattt atgagttgtc tggtctagct tgagtctgtt ccaggcagtg ttgcaccgag
     5401 gggtgaggga ggttaaaggt tcttcgcagt ctctggaaca gcctgggctg ctgccccctg
     5461 agcaacaagg gggccccggg ggagaagaaa aaggggattg atattctagc ccccaataag
     5521 atggtccatg gtgggctaac atacataaat ctggggtaag gtcaggccac caggtccaca
     5581 gagggtggtt gccagaagtt gcccataccg tctcccgatc tccattggtt acctcccagg
     5641 tgatattata gacttgatga ggactggagc cgggcgaagc agtactgacc cctctgagca
     5701 tcagaagaat taaggggatt agggggcctc gcgggttaac cttattttta aggggttttg
     5761 agagcgttga acgcgccatg tcagtctaga ggatggtcca cccccggggt cggcagcctt
     5821 cacgtgggcg gcgtgtatcc aagctgcgat gccgtctact ttgagggcgg tgggggtggt
     5881 cagcaggact gtgtaaggtc ctttccagcg aggttctagg ttcttagtct ggtgtcggcg
     5941 gacccacact gtgtcgccga ctcggtaagg gtgaggtacc accggtcggt ccagttgttc
     6001 ttggtaggct gccgccagag gtctccagac ttcgtgctgg actaagtaga gagcctgtaa
     6061 gtgagcttgg agagaggggc tgttagtaac tcttgtcatg tcagggtcag ggaagtttac
     6121 aaggggcggg ggtgccccat ataagatctc atatggggtg aggccatggg ggcccggcgt
     6181 gttgcgggct cggtacaggg ctaaggggag taggagcacc cagtctctag agccagttgc
     6241 aagcgttaat ttagttaaag tctccttgat ggttctattc attctttcta cctggcctga
     6301 gctttggggt ctgtatgcac aatgtaattt ccaatcaatc cccaacagat cggccactgt
     6361 ctgactcacc ttggagacga aggcaggccc attgtcagtt cccaatacct gaggcatgcc
     6421 gaacctgggg aagatctcct ctagtagctt cttggttacg accttggcgg tttctttctt
     6481 ggttgggaag gcttctatcc agccagaaaa ggtatctata aaaactagaa gatatttata
     6541 gccatacaat ccgggcttta tctcggtgaa atcgatctcc caatgagtgc cgggccgatg
     6601 cccgcggacc ctagttccct gtttaacggc agacttgctg gcgttgactt gtgcacaagc
     6661 tttgcaggtc tcagtgatat ttttgagtgt tcgatcccgg ttcagcatgt agtagggact
     6721 gtggcttctc tctaggagag ccttcatttt tgagaagctg aggtgagtca gctgatgaag
     6781 aaagtctaat aattcaaaag taaactggtc aggcatcaca ggttttcctt ggtagaccca
     6841 atacttcttt gttttatcat aaatggcccc caacttggtt aggtccttta tatcagtcac
     6901 tgtgtaatga aaatgttctg aggtgtaggg tgatgaattt tctatgagga gggtagaggt
     6961 gtctggagtc tctgtgatgg ctgcctttcg ggccgcttgg tcagccatcc ggttgcctct
     7021 agcctcggcg ctgtgtccct tttgatgtcc tggacaatgg attatgctaa gtcttttggg
     7081 cagaaagagg gcttttagta gggccaagat ctcgtcttta tttttgatct ctttgccttc
     7141 tgatgtgagc aacccacgcc ttctgtatat ttctccatgg atatgggcag tagcaaaagc
     7201 ataacggcta tcagtataaa catttagctt cttaccttct gccatcttta gggcctgggt
     7261 gagtgctatc agttcagccc gctgagcgga tgtcccggct ggcagggctt tagcccagat
     7321 tacctcggtc tcggtggtca ccgcagctcc cgccttacgc tgtccctctt gtaagagact
     7381 gcttccatcc gtgtaccagg tgtggtcggc gtctgggagc ggctggtccg ttaggtcggg
     7441 tcgggttccg tgggcttcgg ccaggatatc aaggcagttg tgttgcagcc cttcctcagg
     7501 cagtgggagc agcgtagccg ggttcagggc taccaccggt ccgaactgga cccggtccgt
     7561 gtccaaaagc aaggcctgat agtgagtcat ccgggcgttg gaaagccagc ggtcgggggg
     7621 ttgtttgact agtgcctcta ctgcatgggg ggccagaatg actagtggct gtcccatggt
     7681 tagcttgcct gcatcctttg tcagtacggc aatggctgct accatccgta ggcaaggggg
     7741 ccacccagct gctactgggt ctagcttttt ggacaggtag gccaccggcc gacgccaagg
     7801 tcccagtttt tgcgttagga cacctttggc gtagccctgc ttctcgtcga caaagagttc
     7861 aaagggctta gtcaaatctg gcaaccccag ggctggggca gttagaagag cttgcttgat
     7921 ttcttgatag gccttttgtt ggtctgggcc ccaattaaac agagtccccg ttttggtgag
     7981 agggtacaag ggggctgcca tttctgcaaa cccagggatc cagaggcgac agaagcctgc
     8041 cgtccctagg aactccctta gttgtcgagg ggtcttcgga gtaggctgcc ccatcacagt
     8101 ctcttttctg gcctcagtca gccatctctg accctctttt agaagatacc ccagatactt
     8161 gacctgtttc tggcaaattt gggctttctt ggccgaggcc cgatacccga ggttccctag
     8221 ggtttgtaac agggcccgag taccttgttg gcagtctagc tcagaagtgg cggccagcag
     8281 taagtcatcc acgtactgta gcaggatcaa gtctgggtgc tggatccgga agtctgctag
     8341 gtctctgtgc agtgcctcat caaacagggt gggactgttt ttgaaaccct gtgggagtct
     8401 ggtccaggtc aattgtcctg agattcccat ctctggatct ctccactcaa aggcgaagag
     8461 aggctgactg gtggggtgga gtctcaggca gaaaaaggca tcctttaaat caagcacagt
     8521 gtaccactgg tgggacggtg ggagcccgct caagaggttg taagggttgg gcacggtggg
     8581 gtggatgtct tccacccgct tgttgacttc tctcagatcc tggacaggcc tataatcatt
     8641 agtccctggt ttcttaacgg gtagcagggg cgtgttccag ggggactggc agggtaccag
     8701 tattccctgg tccaacagtc tctgtatgtg gggcttgatc cccagtctgg cttcttgtga
     8761 catggggtat tgttttatgg acacgggggt agaggttgct ttcagaggta tgatcagagg
     8821 agcttggcga actgccagtc ccatgccccc ggtttccgcc caggcctgag gaaaatcaga
     8881 cagccatgtg gaccctagag aaacatctgg ctcttttgag gtctcatgta gccgatactc
     8941 atcttctata tttagggtca acacttgcag gggctgcccc attggtccca taacctgagc
     9001 tcctgatccc tcaaagtgga tttgggcttt tagtttagtc agcaaatctc ttcctaacag
     9061 aggataggga cagtctggta catggaggaa agagtgggtg accttaccgg tagctagatg
     9121 tactttgcga tccgtggtcc agcgataccg ctttcctcca gtagcccctt ggacccaggc
     9181 agacttatca cttaggggtc caggattttg ggtcagcacg gagtgttggg ccccagtatc
     9241 taccaggaag gtgacgggtt gccccccgac tttgagggtt atcctgggtt cagggggggg
     9301 ctcctgaccc tgacctccct agtcatctag ggtcaggagg gaggtctggg gtcttggtcc
     9361 ccgaggtcct cgtggtttct tgggacaatc tttagcccag tgcccctttt ctttgcagta
     9421 ggcacactgg tcgcgatcga gttgggacct ccttcgttct cctccctgtc tatcctgttt
     9481 ctgtccacta acgacagtgg ccaatagctt gctcatctct ctatgtctcc tacgatctct
     9541 ttctttctct ttctgctcat cctctgtcct acggcgttct tctttttcct ctgtttctct
     9601 cctgatacgt tcctctcttt cttccggggt ttctcgttta ttaaagatct tttctgcctc
     9661 tctaaccaaa tctccaagcg tcttgttttt taaatcttct aacctctcta actttctccc
     9721 aatgtctggg gcagactgcc aaatgaaaga catagacaca ttagtttctt gccctgggtc
     9781 ctcagggtca taaggagtgt acctgcgata ggcttcctta agtctctcta ggaaggccga
     9841 gggagactca ttgggccctt gtgttattcc ttttaccttg gccaaattgg tggggcttct
     9901 gcccgcgttt tggagacccg ctaggagcaa ctggcgatag tggactaggt ggttcctacc
     9961 tgcctgggtg gtgtaatccc agtctgggcg ctcgagggga aaagcggcat cgacttcatt
    10021 gggcagttga gtggggcgcc catcatcgcc ccgcaccgcc tttctagcct ctaagagcac
    10081 ccgttgtttt tcttctccgg tcagcagagt ccccaacagc tgctgacagt cgtcccaggt
    10141 gggctgatgg gtgatgagga cagactcgat cagagctgtc agtttacctg gatcttcaga
    10201 aaaagaaggg ttattatttt tccagttgta aaggtcagaa gaggagaacg gccagtattg
    10261 aagctgtccg tttcctcctg cgcggagggg gaatgcctgc gaggtagtgg agtcggccac
    10321 agggggctcc cgtctcccac gtaggcgaga tgccattggg gaggggtccg gtgcctctcc
    10381 cgcaggggtc gcttctccac catttccgtc cctgtcggaa gggggtggtc ttgggtccct
    10441 ataaggcggg gggtcttctg taagtaggtc gatgagcggc cccccactgt cagaaagaac
    10501 ttgaggttta ggtttggcgc ctagagaagg agtgagggct ggataaaggg aggatcgagg
    10561 cggggtcgaa cgaggaggtt caagggggag agacggggcg gatggaggaa gaggaggcgg
    10621 aggcttaggg tgtacaaagg gcttgaccca gggagggggg tcaaaagcca aggcttccca
    10681 ggtcacgatg taggggacct ggtctgggtg tccatgcggg ccaggtgaaa agaccttgat
    10741 cttaacctgg gtgatgaggt ctcggttaaa ggtgccgtct cgcggccatc cgacgttaaa
    10801 ggttggccat tctgcagagc agaaggtaac ccaacgtctc ttcttgacat ctaccgactg
    10861 gttgtgagcg atccgctcga catctttcca gtgacctaag gtcaaactta agggagtggt
    10921 aacagtctgg cccatatttt cagacaaata cagaaacaca gtcagacaga gacaacacag
    10981 aacgatgccc aagcttggcc agcttacctc ccggtggtgg gtcggtggtc cctgggcagg
    11041 ggtctcccga tcccggacga gcccccaaat gaaagacccc cgctgacggg tagtcaatca
    11101 ctcagaggag accctcccaa ggaacagcga gaccacaagt cggatgcaac tgcaagaggg
    11161 tttattggat acacgggtac ccgggcgact cagtcaatcg gaggactggc gcgccgagtg
    11221 aggggttgtg ggctctttta ttgagctctg cttatataga cctcccaccg tacacgccta
    11281 ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt
    11341 tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag
    11401 tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat ggtaatagcg
    11461 atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca
    11521 taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga
    11581 tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa
    11641 tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg
    11701 gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc
    11761 atatatgggc tatgaactaa tgaccccgta attgattact attaataact agtcaataat
    11821 caatgtcaac gcgctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
    11881 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
    11941 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
    12001 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
    12061 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
    12121 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
    12181 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
    12241 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
    12301 aaccctgata aatgcttcaa taatattgaa aaaggaagag t
//
