LOCUS       Exported               14674 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Bacterial genome editing plasmid with spectinomycin resistance 
            marker and using sacB as a counter-selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    pCpf1b-sp
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14674)
  AUTHORS   Niu TC, Lin GM, Xie LR, Wang ZQ, Xing WY, Zhang JY, Zhang CC
  TITLE     Expanding the potential of CRISPR-Cpf1 based genome editing 
            technology in the cyanobacterium Anabaena PCC 7120.
  JOURNAL   ACS Synth Biol. 2018 Dec 10. doi: 10.1021/acssynbio.8b00437.
  PUBMED    30525474
REFERENCE   2  (bases 1 to 14674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14674
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..446
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             447..1868
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     primer_bind     2359..2378
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        2482..2511
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        6435..6469
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     CDS             complement(6537..6833)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSG"
     primer_bind     6683..6705
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6697..6714
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6698..6714
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6724..6743
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6724..6742
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6790..6810)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6790..6808)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6829..6845)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6829..6845)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6842..6864)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6853..6869
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6877..6907)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6922..6943
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             7653..8444
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9223..9617
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            9959..10046
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             11285..12256
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             12767..13606
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
ORIGIN
        1 cacatatacc tgccgttcac tattatttag tgaaatgaga tattatgata ttttctgaat
       61 tgtgattaaa aaggcaactt tatgcccatg caacagaaac tataaaaaat acagagaatg
      121 aaaagaaaca gatagatttt ttagttcttt aggcccgtag tctgcaaatc cttttatgat
      181 tttctatcaa acaaaagagg aaaatagacc agttgcaatc caaacgagag tctaatagaa
      241 tgaggtcgaa aagtaaatcg cgcgggtttg ttactgataa agcaggcaag acctaaaatg
      301 tgtaaagggc aaagtgtata ctttggcgtc accccttaca tattttaggt ctttttttat
      361 tgtgcgtaac taacttgcca tcttcaaaca ggagggctgg aagaagcaga ccgctaacac
      421 agtacataaa aaaggagaca tgaacgatga acatcaaaaa gtttgcaaaa caagcaacag
      481 tattaacctt tactaccgca ctgctggcag gaggcgcaac tcaagcgttt gcgaaagaaa
      541 cgaaccaaaa gccatataag gaaacatacg gcatttccca tattacacgc catgatatgc
      601 tgcaaatccc tgaacagcaa aaaaatgaaa aatatcaagt tcctgaattc gattcgtcca
      661 caattaaaaa tatctcttct gcaaaaggcc tggacgtttg ggacagctgg ccattacaaa
      721 acgctgacgg cactgtcgca aactatcacg gctaccacat cgtctttgca ttagccggag
      781 atcctaaaaa tgcggatgac acatcgattt acatgttcta tcaaaaagtc ggcgaaactt
      841 ctattgacag ctggaaaaac gctggccgcg tctttaaaga cagcgacaaa ttcgatgcaa
      901 atgattctat cctaaaagac caaacacaag aatggtcagg ttcagccaca tttacatctg
      961 acggaaaaat ccgtttattc tacactgatt tctccggtaa acattacggc aaacaaacac
     1021 tgacaactgc acaagttaac gtatcagcat cagacagctc tttgaacatc aacggtgtag
     1081 aggattataa atcaatcttt gacggtgacg gaaaaacgta tcaaaatgta cagcagttca
     1141 tcgatgaagg caactacagc tcaggcgaca accatacgct gagagatcct cactacgtag
     1201 aagataaagg ccacaaatac ttagtatttg aagcaaacac tggaactgaa gatggctacc
     1261 aaggcgaaga atctttattt aacaaagcat actatggcaa aagcacatca ttcttccgtc
     1321 aagaaagtca aaaacttctg caaagcgata aaaaacgcac ggctgagtta gcaaacggcg
     1381 ctctcggtat gattgagcta aacgatgatt acacactgaa aaaagtgatg aaaccgctga
     1441 ttgcatctaa cacagtaaca gatgaaattg aacgcgcgaa cgtctttaaa atgaacggca
     1501 aatggtacct gttcactgac tcccgcggat caaaaatgac gattgacggc attacgtcta
     1561 acgatattta catgcttggt tatgtttcta attctttaac tggcccatac aagccgctga
     1621 acaaaactgg ccttgtgtta aaaatggatc ttgatcctaa cgatgtaacc tttacttact
     1681 cacacttcgc tgtacctcaa gcgaaaggaa acaatgtcgt gattacaagc tatatgacaa
     1741 acagaggatt ctacgcagac aaacaatcaa cgtttgcgcc aagcttcctg ctgaacatca
     1801 aaggcaagaa aacatctgtt gtcaaagaca gcatccttga acaaggacaa ttaacagtta
     1861 acaaataaaa acgcaaaaga aaatgccgat atcctattgg cattttcttt tatttcttat
     1921 caacataaag gtgaatccca tatgaactat ataaaagcag gcaaatggct aaccgtattc
     1981 ctaacctttt ggtaatgact ccaacttatt gatagtgttt tatgttcaga taatgcccga
     2041 tgactttgtc atgcagcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     2101 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     2161 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     2221 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     2281 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     2341 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     2401 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     2461 cgatgtaacc cactcgtgca ctttacactt tatgcttccg gctcgtatgt taggaggtct
     2521 ttatcatgtc aatttatcaa gaatttgtta ataaatatag tttaagtaaa actctaagat
     2581 ttgagttaat cccacagggt aaaacacttg aaaacataaa agcaagaggt ttgattttag
     2641 atgatgagaa aagagctaaa gactacaaaa aggctaaaca aataattgat aaatatcatc
     2701 agttttttat agaggagata ttaagttcgg tttgtattag cgaagattta ttacaaaact
     2761 attctgatgt ttattttaaa cttaaaaaga gtgatgatga taatctacaa aaagatttta
     2821 aaagtgcaaa agatacgata aagaaacaaa tatctgaata tataaaggac tcagagaaat
     2881 ttaagaattt gtttaatcaa aaccttatcg atgctaaaaa agggcaagag tcagatttaa
     2941 ttctatggct aaagcaatct aaggataatg gtatagaact atttaaagcc aatagtgata
     3001 tcacagatat agatgaggcg ttagaaataa tcaaatcttt taaaggttgg acaacttatt
     3061 ttaagggttt tcatgaaaat agaaaaaatg tttatagtag caatgatatt cctacatcta
     3121 ttatttatag gatagtagat gataatttgc ctaaatttct agaaaataaa gctaagtatg
     3181 agagtttaaa agacaaagct ccagaagcta taaactatga acaaattaaa aaagatttgg
     3241 cagaagagct aacctttgat attgactaca aaacatctga agttaatcaa agagtttttt
     3301 cacttgatga agtttttgag atagcaaact ttaataatta tctaaatcaa agtggtatta
     3361 ctaaatttaa tactattatt ggtggtaaat ttgtaaatgg tgaaaataca aagagaaaag
     3421 gtataaatga atatataaat ctatactcac agcaaataaa tgataaaaca ctcaaaaaat
     3481 ataaaatgag tgttttattt aagcaaattt taagtgatac agaatctaaa tcttttgtaa
     3541 ttgataagtt agaagatgat agtgatgtag ttacaacgat gcaaagtttt tatgagcaaa
     3601 tagcagcttt taaaacagta gaagaaaaat ctattaaaga aacactatct ttattatttg
     3661 atgatttaaa agctcaaaaa cttgatttga gtaaaattta ttttaaaaat gataaatctc
     3721 ttactgatct atcacaacaa gtttttgatg attatagtgt tattggtaca gcggtactag
     3781 aatatataac tcaacaaata gcacctaaaa atcttgataa ccctagtaag aaagagcaag
     3841 aattaatagc caaaaaaact gaaaaagcaa aatacttatc tctagaaact ataaagcttg
     3901 ccttagaaga atttaataag catagagata tagataaaca gtgtaggttt gaagaaatac
     3961 ttgcaaactt tgcggctatt ccgatgatat ttgatgaaat agctcaaaac aaagacaatt
     4021 tggcacagat atctatcaaa tatcaaaatc aaggtaaaaa agacctactt caagctagtg
     4081 cggaagatga tgttaaagct atcaaggatc ttttagatca aactaataat ctcttacata
     4141 aactaaaaat atttcatatt agtcagtcag aagataaggc aaatatttta gacaaggatg
     4201 agcattttta tctagtattt gaggagtgct actttgagct agcgaatata gtgcctcttt
     4261 ataacaaaat tagaaactat ataactcaaa agccatatag tgatgagaaa tttaagctca
     4321 attttgagaa ctcgactttg gctaatggtt gggataaaaa taaagagcct gacaatacgg
     4381 caattttatt tatcaaagat gataaatatt atctgggtgt gatgaataag aaaaataaca
     4441 aaatatttga tgataaagct atcaaagaaa ataaaggcga gggttataaa aaaattgttt
     4501 ataaactttt acctggcgca aataaaatgt tacctaaggt tttcttttct gctaaatcta
     4561 taaaatttta taatcctagt gaagatatac ttagaataag aaatcattcc acacatacaa
     4621 aaaatggtag tcctcaaaaa ggatatgaaa aatttgagtt taatattgaa gattgccgaa
     4681 aatttataga tttttataaa cagtctataa gtaagcatcc ggagtggaaa gattttggat
     4741 ttagattttc tgatactcaa agatataatt ctatagatga attttataga gaagttgaaa
     4801 atcaaggcta caaactaact tttgaaaata tatcagagag ctatattgat agcgtagtta
     4861 atcagggtaa attgtaccta ttccaaatct ataataaaga tttttcagct tatagcaaag
     4921 ggcgaccaaa tctacatact ttatattgga aagcgctgtt tgatgagaga aatcttcaag
     4981 atgtggttta taagctaaat ggtgaggcag agctttttta tcgtaaacaa tcaataccta
     5041 aaaaaatcac tcacccagct aaagaggcaa tagctaataa aaacaaagat aatcctaaaa
     5101 aagagagtgt ttttgaatat gatttaatca aagataaacg ctttactgaa gataagtttt
     5161 tctttcactg tcctattaca atcaatttta aatctagtgg agctaataag tttaatgatg
     5221 aaatcaattt attgctaaaa gaaaaagcaa atgatgttca tatattaagt atagatagag
     5281 gtgaaagaca tttagcttac tatactttgg tagatggtaa aggcaatatc atcaaacaag
     5341 atactttcaa catcattggt aatgatagaa tgaaaacaaa ctaccatgat aagcttgctg
     5401 caatagagaa agatagggat tcagctagga aagactggaa aaagataaat aacatcaaag
     5461 agatgaaaga gggctatcta tctcaggtag ttcatgaaat agctaagcta gttatagagt
     5521 ataatgctat tgtggttttt gaggatttaa attttggatt taaaagaggg cgtttcaagg
     5581 tagagaagca ggtctatcaa aagttagaaa aaatgctaat tgagaaacta aactatctag
     5641 ttttcaaaga taatgagttt gataaaactg ggggagtgct tagagcttat cagctaacag
     5701 caccttttga gacttttaaa aagatgggta aacaaacagg tattatctac tatgtaccag
     5761 ctggttttac ttcaaaaatt tgtcctgtaa ctggttttgt aaatcagtta tatcctaagt
     5821 atgaaagtgt cagcaaatct caagagttct ttagtaagtt tgacaagatt tgttataacc
     5881 ttgataaggg ctattttgag tttagttttg attataaaaa ctttggtgac aaggctgcca
     5941 aaggcaagtg gactatagct agctttggga gtagattgat taactttaga aattcagata
     6001 aaaatcataa ttgggatact cgagaagttt atccaactaa agagttggag aaattgctaa
     6061 aagattattc tatcgaatat gggcatggcg aatgtatcaa agcagctatt tgcggtgaga
     6121 gcgacaaaaa gttttttgct aagctaacta gtgtcctaaa tactatctta caaatgcgta
     6181 actcaaaaac aggtactgag ttagattatc taatttcacc agtagcagat gtaaatggca
     6241 atttctttga ttcgcgacag gcgccaaaaa atatgcctca agatgctgat gccaatggtg
     6301 cttatcatat tgggctaaaa ggtctgatgc tactaggtag gatcaaaaat aatcaagagg
     6361 gcaaaaaact caatttggtt atcaaaaatg aagagtattt tgagttcgtg cagaatagga
     6421 ataactaaga attcttgaca gctagctcag tcctaggtat aatgctagcg ctgatttagg
     6481 caaaaacggg tctaagaact ttaaataatt tctactgttg tagatgagag caggtgtcag
     6541 ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg
     6601 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     6661 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc
     6721 gcgtaatacg actcactata gggcgaattg ggtaccgggc cccccctcga ggtcctccag
     6781 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     6841 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6901 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6961 gcccgctttc cacccacctg caaaagtcta agaactttaa ataatttcta ctgttgtaga
     7021 tgctactatt cctgtgcctt cagataattc agtctaagaa ctttaaataa tttctactgt
     7081 tgtagatgtc tagagccttt tgtattagta gccggtctaa gaactttaaa taatttctac
     7141 tgttgtagat tagcgattta tgaaggtcat ttttttgtct agctttaatg cggtagttgg
     7201 taccgtcgat ggcagaaatt cgatatctag atctcatgga tccgcaatgg caacaacgtt
     7261 gcgctcgact ctagaagacc gaaaccttgc gctcgttcgc cagccaggac agaaatgcct
     7321 cgacttcgct gctgcccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac
     7381 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
     7441 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     7501 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     7561 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     7621 agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat
     7681 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     7741 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
     7801 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     7861 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     7921 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     7981 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     8041 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     8101 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     8161 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     8221 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     8281 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     8341 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     8401 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta acaattcgtt
     8461 caagccgacg ccgcttcgcg gcgcggctta actcaagcgt tagatgcact aagcacataa
     8521 ttgctcacag ccaaactatc aggtcaagtc tgcttttatt atttttaagc gtgcataata
     8581 agccctacac aaattgggag atatatcatg aaaggctggc tttttcttgt tatcgcaata
     8641 gttggcgaag taatcgcaac atccgcatta aaatctagcg agggctttac taagctgatc
     8701 cggtggatga ccttttgaat gacctttaat agattatatt actaattaat tggggaccct
     8761 agaggtcccc ttttttattt taaaaatttt ttcacaaaac ggtttacaag cataaagctt
     8821 gctcaatctg aaagcgacca ggtgctcggc gtggcaagac tcgcagcgaa cccgtagaaa
     8881 gccatgctcc agccgcccgc attggagaaa ttcttcaaat tcccgttgca catagcccgg
     8941 caattccttt ccctgctctg ccataagcgc agcgaatgcc gggtaatact cgtcaacgat
     9001 ctgatagaga agggtttgct cgggtcggtg gctctggtaa cgaccagtat cccgatcccg
     9061 gctggccgtc ctggccgcca catgaggcat gttccgcgtc cttgcaatac tgtgtttaca
     9121 tacagtctat cgcttagcgg aaagttcttt taccctcagc cgaaatgcct gccgttgcta
     9181 gacattgcca gccagtgccc gtcactcccg tactaactgt cacgaacccc tgcaataact
     9241 gtcacgcccc cctgcaataa ctgtcacgaa cccctgcaat aactgtcacg cccccaaacc
     9301 tgcaaaccca gcaggggcgg gggctggcgg ggtgttggaa aaatccatcc atgattatct
     9361 aagaataatc cactaggcgc ggttatcagc gcccttgtgg ggcgctgctg cccttgccca
     9421 atatgcccgg ccagaggccg gatagctggt ctattcgctg cgctaggcta cacaccgccc
     9481 caccgctgcg cggcaggggg aaaggcgggc aaagcccgct aaaccccaca ccaaaccccg
     9541 cagaaatacg ctggagcgct tttagccgct ttagcggcct ttccccctac ccgaagggtg
     9601 ggggcgcgtg tgcagccccg cagggcctgt ctcggtcgat cattcagccc ggctcatcct
     9661 tctggcgtgg cggcagaccg aacaaggcgc ggtcgtggtc gcgttcaagg tacgcatcca
     9721 ttgccgccat gagccgatcc tccggccact cgctgctgtt caccttggcc aaaatcatgg
     9781 cccccaccag caccttgcgc cttgtttcgt tcttgcgctc ttgctgctgt tcccttgccc
     9841 gcacccgctg aatttcggca ttgattcgcg ctcgttgttc ttcgagcttg gccagccgat
     9901 ccgccgcctt gttgctcccc ttaaccatct tgacacccca ttgttaatgt gctgtctcgt
     9961 aggctatcat ggaggcacag cggcggcaat cccgacccta ctttgtaggg gagggcgcac
    10021 ttaccggttt ctcttcgaga aactggccta acggccaccc ttcgggcggt gcgctctccg
    10081 agggccattg catggagccg aaaagcaaaa gcaacagcga ggcagcatgg cgatttatca
    10141 ccttacggcg aaaaccggca gcaggtcggg cggccaatcg gccagggcca aggccgacta
    10201 catccagcgc gaaggcaagt atgcccgcga catggatgaa gtcttgcacg ccgaatccgg
    10261 gcacatgccg gagttcgtcg agcggcccgc cgactactgg gatgctgccg acctgtatga
    10321 acgcgccaat gggcggctgt tcaaggaggt cgaatttgcc ctgccggtcg agctgaccct
    10381 cgaccagcag aaggcgctgg cgtccgagtt cgcccagcac ctgaccggtg ccgagcgcct
    10441 gccgtatacg ctggccatcc atgccggtgg cggcgagaac ccgcactgcc acctgatgat
    10501 ctccgagcgg atcaatgacg gcatcgagcg gcccgccgct cagtggttca agcggtacaa
    10561 cggcaagacc ccggagaagg gcggggcaca gaagaccgaa gcgctcaagc ccaaggcatg
    10621 gcttgagcag acccgcgagg catgggccga ccatgccaac cgggcattag agcgggctgg
    10681 ccacgacgcc cgcattgacc acagaacact tgaggcgcag ggcatcgagc gcctgcccgg
    10741 tgttcacctg gggccgaacg tggtggagat ggaaggccgg ggcatccgca ccgaccgggc
    10801 agacgtggcc ctgaacatcg acaccgccaa cgcccagatc atcgacttac aggaataccg
    10861 ggaggcaata gaccatgaac gcaatcgaca gagtgaagaa atccagaggc atcaacgagt
    10921 tagcggagca gatcgaaccg ctggcccaga gcatggcgac actggccgac gaagcccggc
    10981 aggtcatgag ccagacccag caggccagcg aggcgcaggc ggcggagtgg ctgaaagccc
    11041 agcgccagac aggggcggca tgggtggagc tggccaaaga gttgcgggag gtagccgccg
    11101 aggtgagcag cgccgcgcag agcgcccgga gcgcgtcgcg ggggtggcac tggaagctat
    11161 ggctaaccgt gatgctggct tccatgatgc ctacggtggt gctgctgatc gcatcgttgc
    11221 tcttgctcga cctgacgcca ctgacaaccg aggacggctc gatctggctg cgcttggtgg
    11281 cccgatgaag aacgacagga ctttgcaggc cataggccga cagctcaagg ccatgggctg
    11341 tgagcgcttc gatatcggcg tcagggacgc caccaccggc cagatgatga accgggaatg
    11401 gtcagccgcc gaagtgctcc agaacacgcc atggctcaag cggatgaatg cccagggcaa
    11461 tgacgtgtat atcaggcccg ccgagcagga gcggcatggt ctggtgctgg tggacgacct
    11521 cagcgagttt gacttggatg acatgaaagc cgagggccgg gagcctgccc tggtagtgga
    11581 aaccagcccg aagaactatc aggcatgggt caaggtggcc gacgccgcag gcggtgaact
    11641 tcgggggcag attgcccgga cgctggccag cgagtacgac gccgacccgg ccagcgccga
    11701 cagccgccac tatggccgct tggcgggctt caccaaccgc aaggacaagc acaccacccg
    11761 cgccggttat cagccgtggg tgctgctgcg tgaatccaag ggcaagaccg ccaccgctgg
    11821 cccggcgctg gtgcagcagg ctggccagca gatcgagcag gcccagcggc agcaggagaa
    11881 ggcccgcagg ctggccagcc tcgaactgcc cgagcggcag cttagccgcc accggcgcac
    11941 ggcgctggac gagtaccgca gcgagatggc cgggctggtc aagcgcttcg gtgatgacct
    12001 cagcaagtgc gactttatcg ccgcgcagaa gctggccagc cggggccgca gtgccgagga
    12061 aatcggcaag gccatggccg aggccagccc agcgctggca gagcgcaagc ccggccacga
    12121 agcggattac atcgagcgca ccgtcagcaa ggtcatgggt ctgcccagcg tccagcttgc
    12181 gcgggccgag ctggcacggg caccggcacc ccgccagcga ggcatggaca ggggcgggcc
    12241 agatttcagc atgtagtgct tgcgttggta ctcacgcctg ttatactatg agtactcacg
    12301 cacagaaggg ggttttatgg aatacgaaaa aagcgcttca gggtcggtct acctgatcaa
    12361 aagtgacaag ggctattggt tgcccggtgg ctttggttat acgtcaaaca aggccgaggc
    12421 tggccgcttt tcagtcgctg atatggccag ccttaacctt gacggctgca ccttgtcctt
    12481 gttccgcgaa gacaagcctt tcggccccgg caagtttctc ggtgactgat atgaaagacc
    12541 aaaaggacaa gcagaccggc gacctgctgg ccagccctga cgctgtacgc caagcgcgat
    12601 atgccgagcg catgaaggcc aaagggatgc gtcagcgcaa gttctggctg accgacgacg
    12661 aatacgaggc gctgcgcgag tgcctggaag aactcagagc ggcgcagggc gggggtagtg
    12721 accccgccag cgcctaacca ccaactgcct gcaaaggagg caatcaatgg ctacccataa
    12781 gcctatcaat attctggagg cgttcgcagc agcgccgcca ccgctggact acgttttgcc
    12841 caacatggtg gccggtacgg tcggggcgct ggtgtcgccc ggtggtgccg gtaaatccat
    12901 gctggccctg caactggccg cacagattgc aggcgggccg gatctgctgg aggtgggcga
    12961 actgcccacc ggcccggtga tctacctgcc cgccgaagac ccgcccaccg ccattcatca
    13021 ccgcctgcac gcccttgggg cgcacctcag cgccgaggaa cggcaagccg tggctgacgg
    13081 cctgctgatc cagccgctga tcggcagcct gcccaacatc atggccccgg agtggttcga
    13141 cggcctcaag cgcgccgccg agggccgccg cctgatggtg ctggacacgc tgcgccggtt
    13201 ccacatcgag gaagaaaacg ccagcggccc catggcccag gtcatcggtc gcatggaggc
    13261 catcgccgcc gataccgggt gctctatcgt gttcctgcac catgccagca agggcgcggc
    13321 catgatgggc gcaggcgacc agcagcaggc cagccggggc agctcggtac tggtcgataa
    13381 catccgctgg cagtcctacc tgtcgagcat gaccagcgcc gaggccgagg aatggggtgt
    13441 ggacgacgac cagcgccggt tcttcgtccg cttcggtgtg agcaaggcca actatggcgc
    13501 accgttcgct gatcggtggt tcaggcggca tgacggcggg gtgctcaagc ccgccgtgct
    13561 ggagaggcag cgcaagagca agggggtgcc ccgtggtgaa gcctaagaac aagcacagcc
    13621 tcagccacgt ccggcacgac ccggcgcact gtctggcccc cggcctgttc cgtgccctca
    13681 agcggggcga gcgcaagcgc agcaagctgg acgtgacgta tgactacggc gacggcaagc
    13741 ggatcgagtt cagcggcccg gagccgctgg gcgctgatga tctgcgcatc ctgcaagggc
    13801 tggtggccat ggctgggcct aatggcctag tgcttggccc ggaacccaag accgaaggcg
    13861 gacggcagct ccggctgttc ctggaaccca agtgggaggc cgtcaccgct gatgccatgg
    13921 tggtcaaagg tagctatcgg gcgctggcaa aggaaatcgg ggcagaggtc gatagtggtg
    13981 gggcgctcaa gcacatacag gactgcatcg agcgcctttg gaaggtatcc atcatcgccc
    14041 agaatggccg caagcggcag gggtttcggc tgctgtcgga gtacgccagc gacgaggcgg
    14101 acgggcgcct gtacgtggcc ctgaacccct tgatcgcgca ggccgtcatg ggtggcggcc
    14161 agcatgtgcg catcagcatg gacgaggtgc gggcgctgga cagcgaaacc gcccgcctgc
    14221 tgcaccagcg gctgtgtggc tggatcgacc ccggcaaaac cggcaaggct tccatagata
    14281 ccttgtgcgg ctatgtctgg ccgtcagagg ccagtggttc gaccatgcgc aagcgccgcc
    14341 agcgggtgcg cgaggcgttg ccggagctgg tcgcgctggg ctggacggta accgagttcg
    14401 cggcgggcaa gtacgacatc acccggccca aggcggcagg ctgacccccc ccactctatt
    14461 gtaaacaaga catttttatc ttttatattc aatggcttat tttcctgcta attggtaata
    14521 ccatgaaaaa taccatgctc agaaaaggct taacaatatt ttgaaaaatt gcctactgag
    14581 cgctgccgca cagctccata ggccgctttc ctggctttgc ttccagatgt atgctcttct
    14641 gctctagcta gaggatcgat cctttttaac ccat
//
