LOCUS       Exported                4010 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Vector to measure enhancer activity of candidates from a DNA library
            by STARR-seq..
ACCESSION   .
VERSION     .
KEYWORDS    hSTARR-seq_ORI vector
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Muerdter F, Boryn LM, Woodfin AR, Neumayr C, Rath M, Zabidi MA, 
            Pagani M, Haberle V, Kazmar T, Catarino RR, Schernhuber K, Arnold 
            CD, Stark A
  TITLE     Resolving systematic errors in widely used enhancer activity assays 
            in human cells.
  JOURNAL   Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534.
  PUBMED    29256496
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(630..1218)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(710..729)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     intron          1314..1446
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1429..1448
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1581..1611
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1665..2324
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1731..1750)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2666..2971
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2850..2869
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     polyA_signal    3049..3170
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3060..3079)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3114..3133
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3415..3432)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             3590..440
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gaggccctaa ggagctgact gcatttcttc ataatatggg tgatcatgtg acccggcttg
       61 accgctggga accagagttg aacgaagcca taccgaacga cgagcgtgat accacgatgc
      121 cagtagcaat ggccacaact cttcggaaac tactcactgg cgaacttctt actctagcat
      181 cacgacagca gctcatagac tggatggagg cggacaaagt agcaggacca cttcttcgct
      241 cggccctccc tgctggctgg ttcattgctg acaaatcggg ggccggtgaa cgcggctctc
      301 gcggcatcat tgctgcgctg gggcctgatg gtaagccctc acgaatcgta gtgatctaca
      361 cgacggggag tcaggccact atggacgaac gaaataggca gatcgctgag atcggtgcct
      421 cactgatcaa gcactggtaa ccactgcagt ggtttagcat ttgcggccgc tgtcagacca
      481 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
      541 ggtgaagatc ctttttgata atctcatgac atgtgagcaa aaggccagca aaaggccagg
      601 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
      661 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
      721 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
      781 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
      841 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
      901 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
      961 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1021 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1081 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1141 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1201 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1261 aacgaaaact cacgttaagg gattttgggg cccggtcgtg aggcactggg caggtaagta
     1321 tcaaggttac aagacaggtt taaggagacc aatagaaact gggcttgtcg agacagagaa
     1381 gactcttgcg tttctgatag gcacctattg gtcttactga catccacttt gcctttctct
     1441 ccacaggtgt ccaccctgca ggcttaagca tggctagcaa aggagaagaa ctcttcactg
     1501 gagttgtccc aattcttgtt gaattagatt gatctagagc atgcaccggt gatatcgcgg
     1561 ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt
     1621 tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     1681 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     1741 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     1801 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     1861 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
     1921 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     1981 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     2041 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
     2101 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     2161 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     2221 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
     2281 atgaattaca acagtactgc gatgagtggc agggcggggc gtaaacgcgt ggatccggct
     2341 tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat
     2401 atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac
     2461 agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc
     2521 ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa
     2581 gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt
     2641 gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag
     2701 ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat
     2761 ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc
     2821 ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc
     2881 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
     2941 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
     3001 tgcaggatat cgtcgacgaa ttcggccggc cgcttcgagc agacatgata agatacattg
     3061 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     3121 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     3181 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     3241 aaaacctcta caaatgtggt aaaatcgata aggatccgac cgatgccctt gagagccttc
     3301 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
     3361 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct
     3421 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3481 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt cttgagagac
     3541 gtactagtaa ccctgataaa tgcttcaata atattgaaaa aggacgagta tgagcatcca
     3601 acattttcgt gtcgcactca ttcccttctt tgcggcattt tgcttgcctg tttttgcaca
     3661 ccccgaaacg ctggtgaaag taaaagatgc tgaagatcaa ctgggtgcaa gagtgggcta
     3721 tatcgaactg gatctcaata gcggcaagat ccttgagtct ttccgccccg aagaacgatt
     3781 cccgatgatg agcactttca aagtactgct atgtggcgcg gtgttgtccc gtatagacgc
     3841 cgggcaagag cagcttggtc gccgtataca ctactcacaa aacgacttgg ttgagtactc
     3901 gccggtcacg gaaaagcatc ttacggatgg catgacggta agagaattgt gtagtgctgc
     3961 cattaccatg agcgacaaca ccgcggccaa cttacttctg acaacgatcg
//
