LOCUS Exported 4386 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses FKBP-CFAST10 in mammalian cells. ACCESSION . VERSION . KEYWORDS pAG241 FKBP-CFAST10 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4386) AUTHORS Tebo AG, Gautier A TITLE A split fluorescent reporter with rapid and reversible complementation. JOURNAL Nat Commun. 2019 Jun 27;10(1):2822. doi: 10.1038/s41467-019-10855-0. PUBMED 31249300 REFERENCE 2 (bases 1 to 4386) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4386 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 6..309 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 310..513 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 464..484 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 560..569 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 569..598 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 605..925 /codon_start=1 /product="human FK506-binding protein FKBP12" /label=FKBP /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL KLE" polyA_signal 1119..1240 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1156..1175) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1210..1229 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1247..1702) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1384..1405) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1596..1615 /label=F1ori-R /note="F1 origin, reverse primer" promoter 1729..1833 /gene="bla" /label=AmpR promoter promoter 1835..2192 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(1858..1878) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2043..2178 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2105..2124 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2227..3021 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2281..2300) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 2891..2910 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3209..3228) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3253..3300 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 3629..4217 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4118..4137 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 61 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 121 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 181 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 481 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag 541 cgctaccgga ctcagatctg ccaccatgga acaaaagctt atttctgaag aggacttgga 601 attcggagtg caggtggaaa ccatctcccc aggagacggg cgcaccttcc ccaagcgcgg 661 ccagacctgc gtggtgcact acaccgggat gcttgaagat ggaaagaaat ttgattcctc 721 ccgggacaga aacaagccct ttaagtttat gctaggcaag caggaggtga tccgaggctg 781 ggaagaaggg gttgcccaga tgagtgtggg tcagagagcc aaactgacta tatctccaga 841 ttatgcctat ggtgccactg ggcacccagg catcatccca ccacatgcca ctctcgtctt 901 cgatgtggag cttctaaaac tggaagaatc cggaggaggc ggcagcggcg gagggggatc 961 cggtgacagc tattgggtct ttgtgaaacg gtaataggcg gccgcgactc tagatcataa 1021 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc 1081 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata 1141 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 1201 attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt 1261 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag 1321 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 1381 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 1441 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 1501 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 1561 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 1621 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 1681 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct 1741 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 1801 taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga 1861 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 1921 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 1981 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 2041 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 2101 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 2161 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg 2221 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg 2281 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg 2341 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat 2401 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca 2461 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg 2521 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat 2581 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa 2641 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg 2701 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg 2761 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg 2821 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat 2881 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac 2941 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc 3001 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc 3061 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg 3121 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt 3181 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa 3241 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc 3301 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt 3361 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag 3421 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat 3481 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 3541 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 3601 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 3661 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 3721 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc 3781 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 3841 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 3901 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 3961 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 4021 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 4081 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 4141 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 4201 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 4261 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 4321 ccgccatgca ttagttatta atagtaatca attacggggt cattagttca tagcccatat 4381 atggag //