LOCUS Exported 9473 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses human cGAS-HA; Puromycin selection marker. ACCESSION . VERSION . KEYWORDS pLenti-CMV-cGAS-HA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9473) AUTHORS Barnett KC, Coronas-Serna JM, Zhou W, Ernandes MJ, Cao A, Kranzusch PJ, Kagan JC TITLE Phosphoinositide Interactions Position cGAS at the Plasma Membrane to Ensure Efficient Distinction between Self- and Viral DNA. JOURNAL Cell. 2019 Mar 7;176(6):1432-1446.e11. doi: 10.1016/j.cell.2019.01.049. Epub 2019 Feb 28. PUBMED 30827685 REFERENCE 2 (bases 1 to 9473) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9473 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..227 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 228..408 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 455..580 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1073..1306 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1491..1535 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 1802..1918 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" enhancer 1941..2244 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2245..2448 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2399..2419 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 2445..2469 /label=LNCX /note="Human CMV promoter, forward primer" CDS 4097..4123 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" misc_feature 4133..4721 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4186..4206) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4604..4615) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" promoter 4735..5234 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(4770..4793) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 5167..5186 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 5255..5854 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(5255..5274) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 5751..5771 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 5992..6225 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 6297..6418 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6334..6353) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6388..6407 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 6458..6593 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6520..6539 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(6613..6632) /label=T7 /note="T7 promoter, forward primer" promoter complement(6614..6632) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(6642..6659) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(6642..6658) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6651..6673) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 6800..7255 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6887..6906) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7097..7118 /label=F1ori-F /note="F1 origin, forward primer" promoter 7281..7385 /gene="bla" /label=AmpR promoter CDS 7386..8246 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7604..7623) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8417..9005 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8906..8925 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9159..9176 /label=L4440 /note="L4440 vector, forward primer" protein_bind 9293..9314 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9329..9359 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9367..9383 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9372..9394 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9391..9407 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9391..9407 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 9426..9446 /label=T3 /note="T3 promoter, forward primer" promoter 9428..9446 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" ORIGIN 1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg 61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc 121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc 181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt 241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc 481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac 541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat 601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa 661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga 721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc 781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat 841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa 901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca 961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac 1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt 1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga 1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg 1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg 1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt 1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat 1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt 1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac 1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt 1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag 1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc 1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg 1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa 1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa 1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat 1921 cgataagctt gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg 1981 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata 2041 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta 2101 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc 2161 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 2221 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2281 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2341 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2401 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2461 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc 2521 tagagccacc atgcagcctt ggcacggaaa ggccatgcag agagcttccg aggccggagc 2581 cactgccccc aaggcttccg cacggaatgc caggggcgcc ccgatggatc ccaccgagtc 2641 tccggctgcc cccgaggccg ccctgcctaa ggcgggaaag ttcggccccg ccaggaagtc 2701 gggatcccgg cagaaaaaga gcgccccgga cacccaggag aggccgcccg tccgcgcaac 2761 tggggcccgc gccaaaaagg cccctcagcg cgcccaggac acgcagccgt ctgacgccac 2821 cagcgcccct ggggcagagg ggctggagcc tcctgcggct cgggagccgg ctctttccag 2881 ggctggttct tgccgccaga ggggcgcgcg ctgctccacg aagccaagac ctccgcccgg 2941 gccctgggac gtgcccagcc ccggcctgcc ggtctcggcc cccattctcg tacggaggga 3001 tgcggcgcct ggggcctcga agctccgggc ggttttggag aagttgaagc tcagccgcga 3061 tgatatctcc acggcggcgg ggatggtgaa aggggttgtg gaccacctgc tgctcagact 3121 gaagtgcgac tccgcgttca gaggcgtcgg gctgctgaac accgggagct actatgagca 3181 cgtgaagatt tctgcaccta atgaatttga tgtcatgttt aaactggaag tccccagaat 3241 tcaactagaa gaatattcca acactcgtgc atattacttt gtgaaattta aaagaaatcc 3301 gaaagaaaat cctctgagtc agtttttaga aggtgaaata ttatcagctt ctaagatgct 3361 gtcaaagttt aggaaaatca ttaaggaaga aattaacgac attaaagata cagatgtcat 3421 catgaagagg aaaagaggag ggagccctgc tgtaacactt cttattagtg aaaaaatatc 3481 tgtggatata accctggctt tggaatcaaa aagtagctgg cctgctagca cccaagaagg 3541 cctgcgcatt caaaactggc tttcagcaaa agttaggaag caactacgac taaagccatt 3601 ttaccttgta cccaagcatg caaaggaagg aaatggtttc caagaagaaa catggcggct 3661 atccttctct cacatcgaaa aggaaatttt gaacaatcat ggaaaatcta aaacgtgctg 3721 tgaaaacaaa gaagagaaat gttgcaggaa agattgttta aaactaatga aatacctttt 3781 agaacagctg aaagaaaggt ttaaagacaa aaaacatctg gataaattct cttcttatca 3841 tgtgaaaact gccttctttc acgtatgtac ccagaaccct caagacagtc agtgggaccg 3901 caaagacctg ggcctctgct ttgataactg cgtgacatac tttcttcagt gcctcaggac 3961 agaaaaactt gagaattatt ttattcctga attcaatcta ttctctagca acttaattga 4021 caaaagaagt aaagaatttc tgacaaagca aattgaatat gaaagaaaca atgagtttcc 4081 agtttttgat gaattttacc catacgatgt tccagattac gcttgagtcg acaatcaacc 4141 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 4201 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 4261 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 4321 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 4381 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 4441 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 4501 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt 4561 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 4621 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 4681 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc taccgggtag 4741 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt 4801 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac 4861 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt 4921 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca 4981 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca 5041 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt 5101 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag 5161 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat 5221 ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc ccacggtgcg 5281 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga 5341 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct 5401 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga 5461 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc 5521 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat 5581 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg 5641 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga 5701 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc 5761 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg 5821 cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc 5881 gaccgaaagg agcgcacgac cccatgcatc tcgagggccc ggtaccttta agaccaatga 5941 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc 6001 tagctcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag 6061 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 6121 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 6181 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc 6241 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact 6301 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 6361 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 6421 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 6481 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 6541 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac 6601 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa 6661 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct 6721 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc 6781 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 6841 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 6901 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 6961 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt 7021 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 7081 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 7141 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag 7201 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg 7261 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 7321 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 7381 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 7441 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 7501 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 7561 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 7621 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 7681 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 7741 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 7801 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 7861 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 7921 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 7981 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 8041 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 8101 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 8161 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 8221 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 8281 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 8341 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 8401 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 8461 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 8521 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 8581 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 8641 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 8701 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 8761 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 8821 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 8881 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 8941 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 9001 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 9061 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 9121 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 9181 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 9241 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga 9301 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt 9361 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca 9421 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taa //