LOCUS Exported 7263 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION AAV plasmid encoding mouse CNDP1 with C-terminal flag tag. ACCESSION . VERSION . KEYWORDS pENN.AAV.CB7.CI.CNDP1-flag.WPRE.rBG SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7263) TITLE Family-wide annotation of physiologic enzyme substrates by in vivo parallel metabolomics JOURNAL Unpublished REFERENCE 2 (bases 1 to 7263) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7263 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR enhancer 204..583 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 585..862 /label=chicken beta-actin promoter intron 863..1879 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 1803..1825 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 1887..1906 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" CDS 3432..3455 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" misc_feature 3486..4074 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3539..3559) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3957..3968) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(4100..4119) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 4165..4220 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" repeat_region 4313..4442 /label=AAV2 ITR primer_bind complement(4461..4478) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(4461..4477) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4470..4492) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 4619..5074 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4706..4725) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4916..4937 /label=F1ori-F /note="F1 origin, forward primer" promoter 5100..5204 /gene="bla" /label=AmpR promoter CDS 5205..6065 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5423..5442) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6236..6824 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6725..6744 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6978..6995 /label=L4440 /note="L4440 vector, forward primer" protein_bind 7112..7133 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7148..7178 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7186..7202 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7191..7213 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 7210..7226 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 7210..7226 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" ORIGIN 1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag ggtaatgggg 181 atcctctaga actatagcta gtcgacattg attattgact agttattaat agtaatcaat 241 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 301 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 361 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 421 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 481 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 541 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac 601 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat 661 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg 721 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca 781 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa 841 aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc 901 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag 961 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt 1021 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg 1081 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc 1141 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt 1201 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa 1261 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt 1321 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg 1381 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca 1441 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg 1501 cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt 1561 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa 1621 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg 1681 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc 1741 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc 1801 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc 1861 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt 1921 tggcaaagaa ttctgcagat atcgccacca tgttctcttc ggcacactct gggctgttgg 1981 agaagctctt ccactacata gacctccacc aggatgagtt cgtgcagaca ctgaaggagt 2041 gggtggccat tgagagtgat tctgtgcagc ctgtgcctcg tctcagacag aagctcttcc 2101 agatgatggc cttggctgca gacaaactcc ggaacctggg agctggggtg gagtccatag 2161 acttgggttc tcagcagatg cctgatggtc agagtctccc catacctcct atcctattgg 2221 ctgagctggg aagtgatccc gagaagccaa ctgtatgctt ctatgggcac ctggacgtgc 2281 agccagctca gaaggacgac gggtggctca cggaccccta cacactgaca gaggtggatg 2341 ggaaactgta tggacgggga gcaacagaca acaagggccc cgtcttggct tggatcaatg 2401 ccgtgagcac cttcagagct ctgcagcagg atcttcccgt gaacatcaaa ctcatcctgg 2461 agggaatgga agaagccggt tccatcgccc tggaggaact cgtcatgaga gaaaaggacc 2521 acttcttctc cagtgtggac tacattgtga tttcagacaa cctgtggctc agccagagga 2581 aacctgcact cacttatgga acccggggga actgctactt cacggtggag gtgaagtgta 2641 gagatcaaga tttccactca ggaacctttg gtggcatcct caacgaacca atggctgact 2701 tggttgctct tctaggtagc ctggtggatt catcaggcca catcctaatt cctggaatct 2761 atgatcaaat ggcacctatt acagagggag aaaagacaat gtataaaaac attgatatgg 2821 acctagaaga ataccagaac atcaaccaag ttgagaaatt tctgtttgat accaaggagg 2881 aacttctgat gcacctgtgg aggtatccgt ctctctccat ccatggcatc gagggtgcct 2941 ttgatgagcc tggaactaaa acagtcattc ccggccgagt cctagggaaa ttttcaatcc 3001 gtctagtccc taccatgtct ccgtctgtgg tggagaaaca ggtaactcag catctcgaag 3061 ctgtcttctc taaaaggaat agtttcaaca agatggccgt ttctatggtc ctaggactcc 3121 acccatggac tgcaaacgtc aatgatactc agtaccttgc agcacagaga accatcaaaa 3181 cagtgtttgg agttaatcct gacatgatcc gggatgggtc aaccattcca attgccaaaa 3241 tattccaggc tatcacacaa aagagtgtga tgatgctccc actgggagct gtggacgatg 3301 gagagcactc tcagaatgag aagatcaaca ggtggaacta catacagggg tcgaaattat 3361 tcgctgcctt tttcctggag ttgtcaaaac agcattcagg acaccagatg ccttccagtg 3421 tctacggagg agactacaag gacgatgacg acaagtagta atgactcgag cctaagctta 3481 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg 3541 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt 3601 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg 3661 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc 3721 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc 3781 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc 3841 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc 3901 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg 3961 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc 4021 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat 4081 accgtcgatg aggatccgat ctttttccct ctgccaaaaa ttatggggac atcatgaagc 4141 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt 4201 ggaatttttt gtgtctctca ctcggaagca attcgttgat ctgaatttcg accacccata 4261 atacccatta ccctggtaga taagtagcat ggcgggttaa tcattaacta caaggaaccc 4321 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga 4381 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc 4441 agccttaatt aacctaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct 4501 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc 4561 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac 4621 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 4681 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 4741 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 4801 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 4861 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 4921 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 4981 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 5041 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc 5101 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac 5161 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt 5221 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag 5281 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg 5341 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa 5401 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc 5461 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag 5521 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa 5581 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc 5641 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg 5701 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa 5761 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa 5821 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg 5881 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag 5941 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg 6001 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt 6061 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt 6121 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 6181 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 6241 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 6301 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 6361 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga 6421 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 6481 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 6541 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 6601 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 6661 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 6721 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 6781 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 6841 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 6901 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca 6961 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca 7021 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg 7081 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac 7141 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac 7201 aatttcacac aggaaacagc tatgaccatg attacgccag atttaattaa ggccttaatt 7261 agg //