LOCUS       Exported                9784 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Production of SIV-VLPs containing Vpx protein.
ACCESSION   .
VERSION     .
KEYWORDS    pSIV-D3psi/delta env/delta Vif/delta Vpr
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9784)
  AUTHORS   Kim K, Dauphin A, Komurlu S, McCauley SM, Yurkovetskiy L, Carbone C,
            Diehl WE, Strambio-De-Castillia C, Campbell EM, Luban J
  TITLE     Cyclophilin A protects HIV-1 from restriction by human TRIM5alpha.
  JOURNAL   Nat Microbiol. 2019 Dec;4(12):2044-2051. doi: 
            10.1038/s41564-019-0592-5. Epub 2019 Oct 21.
  PUBMED    31636416
REFERENCE   2  (bases 1 to 9784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9784
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        32..136
                     /gene="bla"
                     /label=AmpR promoter
     CDS             137..997
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(355..374)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1168..1756
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1657..1676
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1842..1858
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1842..1858
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     enhancer        1955..2334
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2335..2538
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2489..2509
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2535..2559
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    9633..9767
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9670..9689)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9724..9743
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
       61 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      121 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      181 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      241 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      301 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      361 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      421 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      481 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      541 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      601 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      661 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      721 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      781 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      841 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      901 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      961 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1021 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1081 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1141 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1201 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1261 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1321 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1381 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1441 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1501 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1561 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1621 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1681 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1741 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1801 gccttttgct cacatgtggg ccctaggccg gcctcggccg ctctagaact agtggatccg
     1861 ggccgctcta gagagcttgg cccattgcat acgttgtatc catatcataa tatgtacatt
     1921 tatattggct catgtccaac attaccgcca tgttgacatt gattattgac tagttattaa
     1981 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     2041 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     2101 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     2161 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     2221 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     2281 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     2341 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     2401 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     2461 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     2521 gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
     2581 tgttttgacc tccatagaag acaccgggac cgatccagcc tccggtcgag gccgcaagct
     2641 tggcctccgg ttgcaggtaa gtgcaacaca aaaaagaaat agctgtcttg ttatccagga
     2701 agggataata agatagagtg ggagatgggc gcgagaaact ccgtcttgtc agggaagaaa
     2761 gcagatgaat tagaaaaaat taggctacga cccggcggaa agaaaaagta catgttgaag
     2821 catgtagtat gggcagcaaa tgaattagat agatttggat tagcagaaag cctgttggag
     2881 aacaaagaag gatgtcaaaa aatactttcg gtcttagctc cattagtgcc aacaggctca
     2941 gaaaatttaa aaagccttta taatactgtc tgcgtcatct ggtgcattca cgcagaagag
     3001 aaagtgaaac acactgagga agcaaaacag atagtgcaga gacacctagt ggtggaaaca
     3061 ggaacagcag aaactatgcc aaaaacaagt agaccaacag caccatctag cggcagagga
     3121 ggaaattacc cagtacaaca aataggtggt aactatgtcc acctgccatt aagcccgaga
     3181 acattaaatg cctgggtaaa attgatagag gaaaagaaat ttggagcaga agtagtgcca
     3241 ggatttcagg cactgtcaga aggctgcacc ccctatgaca ttaatcagat gttaaattgt
     3301 gtgggagacc atcaagcggc tatgcagatt atcagagata ttataaatga ggaggctgca
     3361 gattgggact tgcagcaccc acaaccagct ccacaacaag gacagcttag ggagccgtca
     3421 ggatcagata ttgcaggaac aactagttca gtagatgaac aaatccagtg gatgtacaga
     3481 caacagaacc ccataccagt aggcaacatt tacaggagat ggatccaact ggggttgcaa
     3541 aaatgtgtca gaatgtataa cccaacaaac attctagatg taaaacaagg gccaaaagag
     3601 ccatttcaga gctatgtaga caggttctac aaaagcttaa gagcagaaca aacagatgca
     3661 gcagtaaaga attggatgac tcaaacactg ctgattcaaa atgctaaccc agattgcaag
     3721 ctagtgctga aggggctggg tgtgaatccc accctagaag aaatgctgac ggcttgtcaa
     3781 ggagtagggg gaccaggaca gaaggctaga ttaatggcag aagccctgaa agaggccctc
     3841 gcaccagtgc caatcccttt tgcagcagcc cagaagaggg gaccaagaaa gccaattaag
     3901 tgttggaatt gtgggaagga gggacactct gcaaggcaat gcagagcccc aagaagacag
     3961 ggatgctgga aatgtggaaa aatggaccat gttatggcca aatgcccaga cagacaggcg
     4021 ggttttttag gccttggtcc atggggaaag aagccccgca atttccccat ggctcaagtg
     4081 catcaggggc tgacgccaac tgctccccca gaggacccag ctgtggatct gctaaagaac
     4141 tacatgcagt tgggcaagca gcagagagaa agcagagaga agccttacaa ggaggtgaca
     4201 gaggatttgc tgcacctcaa ttctctcttt ggaggagacc agtagtcact gctcatattg
     4261 aaggacagcc tgtagaagta ttattggata caggggctga tgattctatt gtaacaggaa
     4321 tagagttagg tccacattat accccaaaaa tagtaggagg aataggaggt tttattaata
     4381 ctaaagaata caaaaatgta aaaatagaag ttttaggcaa aaggattaaa gggacaatca
     4441 tgacagggga cactccgatt aacatttttg gtaggaattt gctaacagct ctggggatgt
     4501 ctctaaatct tcccatagct aaggtagagc ctgtaaaagt caccttaaag ccaggaaagg
     4561 ttggaccaaa attgaagcag tggccattat caaaagaaaa gatagttgca ttaagagaaa
     4621 tctgtgaaaa gatggaaaag gatggtcagt tggaggaagc tcccccgacc aatccataca
     4681 acacccccac atttgccata aagaaaaaag ataagaacaa atggagaatg ctgatagatt
     4741 ttagggaact aaatagggtc actcaggact ttacagaagt ccaattagga ataccacacc
     4801 ctgcaggact agcaaaaagg aaaaggatta cagtactgga tataggtgat gcatatttct
     4861 ccatacctct agatgaagaa tttaggcagt acactgcctt tactttacca tcagtaaata
     4921 atgcagagcc aggaaaacga tacatttata aggttctgcc tcagggatgg aaggggtcac
     4981 cagccatctt ccaatacact atgagacatg tgctagaacc cttcaggaag gcaaatccag
     5041 atgtgacctt agtccagtat atggatgaca tcttaatagc tagtgacagg acagacctgg
     5101 aacatgacag ggtagtttta cagctaaagg aactcttaaa tagcataggg ttctctaccc
     5161 cagaagagaa attccaaaaa gatcccccat ttcaatggat ggggtacgaa ttgtggccga
     5221 caaaatggaa gttgcaaaag atagagttgc cacaaagaga gacctggaca gtgaatgata
     5281 tacagaagtt agtaggagta ttaaattggg cagctcaaat ttatccaggt ataaaaacca
     5341 aacatctctg taggttaatt agaggaaaaa tgactctaac agaggaagtt cagtggactg
     5401 agatggcaga agcagaatat gaggaaaata agataattct cagtcaggaa caagaaggat
     5461 gttattacca agaaggcaag ccattagaag ccacggtaat aaagagtcag gacaatcagt
     5521 ggtcttataa aattcaccaa gaagacaaaa tactgaaagt aggaaaattt gcaaagataa
     5581 agaatacaca taccaatgga gttagactat tagcacatgt aatacagaaa ataggaaagg
     5641 aagcaatagt gatctgggga caggtcccaa aattccactt accagttgag agggatgtat
     5701 gggaacagtg gtggacagac tattggcagg taacctggat accggagtgg gattttatct
     5761 caacgccacc actagtaaga ttagtcttca atctagtgaa ggaccctata gagggagaag
     5821 aaacctatta tacagatgga tcatgtaata aacagtcaaa agaagggaaa gcaggatata
     5881 tcacagatag gggcaaagac aaagtaaaag tgttagaaca gactactaat caacaagcag
     5941 aattagaagc atttctcatg gcattgacag actcagggcc aaagacaaat attatagtag
     6001 attcacaata tgttatggga ataataacag gatgccctac agaatcagag agcaggctag
     6061 ttaaccaaat aatagaagaa atgattaaaa agtcagaaat ttatgtagca tgggtaccag
     6121 cacacaaagg tataggagga aaccaagaaa tagaccacct agttagtcag gggattagac
     6181 aagttctctt cttggaaaag atagagccag cacaagaaga acatgataaa taccatagta
     6241 atgtaaaaga attggtattc aaatttggat tacccagaat agtggccaga cagatagtag
     6301 acacctgtga taaatgtcat cagaaaggag aagctataca tgggcaggta aattcagatc
     6361 tagggacttg gcaaatggac tgtacccatc tagaaggaaa aatagtcata gttgcagtac
     6421 atgtagctag tggattcata gaagcagaag taattccaca agagacagga agacagacag
     6481 cactatttct gttaaaattg gcaggcagat ggcctattac acatctacac acagataatg
     6541 gtgctaactt tgcctcgcaa gaagtaaaga tggttgcatg gtgggcaggg atagagcaca
     6601 cctttggggt accatacaat ccacagagtc agggagtagt ggaagcaatg aatcaccacc
     6661 tgaaaaatca aatagataga atcagggaac aagcaaattc agtagaaacc atagtattaa
     6721 tggcagttca ttgcatgaat tttaaaagaa ggggaggaat aggggatatg actccagcag
     6781 aaagattaat taacatgatc actacagaac aagaaataca atttcaacaa tcaaaaaact
     6841 caaaatttaa aaattttcgg gtctattaca gagaaggcag agatcaactg tggaagggac
     6901 ccggtgagct attgtggaaa ggggaaggag cagtcatctt aaaggtaggg acagacatta
     6961 aggtagtacc cagaagaaag gctaaaatta tcaaagatta cggaggagga aaagaggtgg
     7021 atagcagttc ccacatggag gataccggag aggctagaga ggtggcatag cctcataaaa
     7081 tatctgaaat agaaaactaa agatctacaa aaggtttgct atgtgcccca ttttaaggtc
     7141 ggatgggcat ggtggacctg cagcagagta atcttccccc tacaggaagg aagccattta
     7201 gaagtacaag ggtattggca tttgacacca gaaagagggt ggctcagtac ttatgcagtg
     7261 aggataacat ggtactcaag gaacttttgg acagatgtaa caccagacta tgcagacatt
     7321 ttactgcata gcacttattt cccttgcttt acagcgggag aagtgagaag ggccatcagg
     7381 ggagaacaac tgctgtcttg ctgcaagttc ccgagagcac ataggtacca ggtaccaagc
     7441 ctacagtact tagcactaaa agtagtaagc gatgtcagat cccagggaga gaatcccacc
     7501 tggaaacagt ggagaagaga caataggaga agccttcgaa tggctaaaca gaacagtaga
     7561 ggagataaac agagaggcag taaaccacct accaagggag ctgattttcc aggtttggca
     7621 aaggtcttgg gaatactggc atgatgaaca agggatgtca caaagctatg taaaatacag
     7681 atacttgtgt ttaatgcaaa aggctttatt tatgcattgc aagaaaggct gtagatgtct
     7741 aggggaagga cacggggcag gaggatggag accaggacct cctcctcctc cccctccagg
     7801 actagcataa taggaagaaa gacctccgga aaatgaaggc ccacaaaggg aaccatggga
     7861 tgaatgggta gtggaggttt aggaagaatt gaaagaagaa gctttaaaac attttgatcc
     7921 tcgcttgcta actgcacttg gtaatcatat ctataataga catggagaca cccttgaggg
     7981 agcaggagaa ctcattagaa tcctccaacg agcgctcttc atgcatttta gaggcggatg
     8041 caaccactcc agaatcggcc aacctggggg aggaaatcct ctctcaacta taccgccctc
     8101 ttgaggcgtg ctataacaca tgctattgta aaaagtgttg ctaccattgc cagttttgtt
     8161 ttcttaaaaa gggattgggg atatgttatg agcagtcacg aaagagaaga agaactccga
     8221 aaaaggctaa ggctaataca tcttctgcat caaacaagta agtatgccat tgcatacgtt
     8281 gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac cgccatgttg
     8341 acattgatta ttgactagta tcaccatgag tgcagaggtg gcagaactgt atcgattgga
     8401 gttgggagat tataaattag tagagatcac tccgattggc ttggccccca cagatgtgaa
     8461 gaggtacact actggtggca cctcaagaaa taaaagaggg gtctttgtgc tagggttctt
     8521 gggttttctc gcaacggcag gttctgcaat gggcgcggcg tcgttgacgc tgaccgctca
     8581 gtcccggact ttattggctg ggatagtgca gcaacagcaa cagctgttgg acgtggtcaa
     8641 gagacaacaa gaattgttgc gactgaccgt ctggggaaca aagaacctcc agactagggt
     8701 cactgccatc gagaagtact taaaggacca ggcgcagcta aatgcttggg gatgtgcgtt
     8761 tagacaagtc tgccacacta ctgtaccatg gccaaatgca agtctaacac cagactggaa
     8821 caatgatact tggcaagagt gggagcgaaa ggttgacttc ttggaggaaa atataacagc
     8881 cctcctagaa gaggcacaaa ttcaacaaga gaagaacatg tatgaattac aaaagttgaa
     8941 tagctgggat gtgtttggca attggtttga ccttgcttct tggataaagt atatacaata
     9001 tggaatttat gtagttgtag gagtaatact gttaagaata gtgatctata tagtacaaat
     9061 gctagctaag ttaaggcagg ggtataggcc agtgttctct tccccaccct cttatttcca
     9121 gtagactcat acccaacagg acccggcact gccaaccaga gaaggcaaag aaggagacgg
     9181 tggagaaggc ggtggcaaca gctcctggcc ttggcagata gaatatattc atttcctgat
     9241 ccgccaactg atacgcctct tgacttggct attcagcaac tgcagaacct tgctatcgag
     9301 agcataccag atcctccaac caatactcca gaggctctct gcgaccctac gaagggttcg
     9361 agaagtcctc aggactgaac tgacctacct acaatatggg tggagctatt tccatgaggc
     9421 ggtccaagcc ggctggagat ctgcgacaga aactcttgcg ggcgcgtgga gagacttatg
     9481 ggagactctt aggagaggtg gaagatggat cctcgcaatc cctagagatc ataatcagcc
     9541 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     9601 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     9661 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     9721 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcgaa ttcgatatca
     9781 ttgg
//
