LOCUS       Exported                7333 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Encodes the AAV-DJ capsid with the N589 residue replaced with a TAG 
            stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-DJ-N589X
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Katrekar D, Moreno AM, Chen G, Worlikar A, Mali P
  TITLE     Oligonucleotide conjugated multi-functional adeno-associated 
            viruses.
  JOURNAL   Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.1038/s41598-018-21742-x.
  PUBMED    29483550
REFERENCE   2  (bases 1 to 7333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7333
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..17
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4009..4020
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(4472..4492)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4472..4490)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4511..4527)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4511..4527)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4524..4546)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4535..4551
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4559..4589)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4604..4625
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4742..4759)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4913..5501)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4993..5012)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5672..6532)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6295..6314
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6533..6637)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6663..7118)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6800..6821)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7012..7031
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7245..7267
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7259..7276
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7260..7276
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7286..7305
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7286..7304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     7333..16
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 cgaggtcgac ggtatcgggg gagctcgcag ggtctccatt ttgaagcggg aggtttgaac
       61 gcgcagccgc catgccgggg ttttacgaga ttgtgattaa ggtccccagc gaccttgacg
      121 agcatctgcc cggcatttct gacagctttg tgaactgggt ggccgagaag gaatgggagt
      181 tgccgccaga ttctgacatg gatctgaatc tgattgagca ggcacccctg accgtggccg
      241 agaagctgca gcgcgacttt ctgacggaat ggcgccgtgt gagtaaggcc ccggaggctc
      301 ttttctttgt gcaatttgag aagggagaga gctacttcca catgcacgtg ctcgtggaaa
      361 ccaccggggt gaaatccatg gttttgggac gtttcctgag tcagattcgc gaaaaactga
      421 ttcagagaat ttaccgcggg atcgagccga ctttgccaaa ctggttcgcg gtcacaaaga
      481 ccagaaatgg cgccggaggc gggaacaagg tggtggatga gtgctacatc cccaattact
      541 tgctccccaa aacccagcct gagctccagt gggcgtggac taatatggaa cagtatttaa
      601 gcgcctgttt gaatctcacg gagcgtaaac ggttggtggc gcagcatctg acgcacgtgt
      661 cgcagacgca ggagcagaac aaagagaatc agaatcccaa ttctgatgcg ccggtgatca
      721 gatcaaaaac ttcagccagg tacatggagc tggtcgggtg gctcgtggac aaggggatta
      781 cctcggagaa gcagtggatc caggaggacc aggcctcata catctccttc aatgcggcct
      841 ccaactcgcg gtcccaaatc aaggctgcct tggacaatgc gggaaagatt atgagcctga
      901 ctaaaaccgc ccccgactac ctggtgggcc agcagcccgt ggaggacatt tccagcaatc
      961 ggatttataa aattttggaa ctaaacgggt acgatcccca atatgcggct tccgtctttc
     1021 tgggatgggc cacgaaaaag ttcggcaaga ggaacaccat ctggctgttt gggcctgcaa
     1081 ctaccgggaa gaccaacatc gcggaggcca tagcccacac tgtgcccttc tacgggtgcg
     1141 taaactggac caatgagaac tttcccttca acgactgtgt cgacaagatg gtgatctggt
     1201 gggaggaggg gaagatgacc gccaaggtcg tggagtcggc caaagccatt ctcggaggaa
     1261 gcaaggtgcg cgtggaccag aaatgcaagt cctcggccca gatagacccg actcccgtga
     1321 tcgtcacctc caacaccaac atgtgcgccg tgattgacgg gaactcaacg accttcgaac
     1381 accagcagcc gttgcaagac cggatgttca aatttgaact cacccgccgt ctggatcatg
     1441 actttgggaa ggtcaccaag caggaagtca aagacttttt ccggtgggca aaggatcacg
     1501 tggttgaggt ggagcatgaa ttctacgtca aaaagggtgg agccaagaaa agacccgccc
     1561 ccagtgacgc agatataagt gagcccaaac gggtgcgcga gtcagttgcg cagccatcga
     1621 cgtcagacgc ggaagcttcg atcaactacg cagacaggta ccaaaacaaa tgttctcgtc
     1681 acgtgggcat gaatctgatg ctgtttccct gcagacaatg cgagagaatg aatcagaatt
     1741 caaatatctg cttcactcac ggacagaaag actgtttaga gtgctttccc gtgtcagaat
     1801 ctcaacccgt ttctgtcgtc aaaaaggcgt atcagaaact gtgctacatt catcatatca
     1861 tgggaaaggt gccagacgct tgcactgcct gcgatctggt caatgtggat ttggatgact
     1921 gcatctttga acaataaatg atttaaatca ggtatggctg ccgatggtta tcttccagat
     1981 tggctcgagg acactctctc tgaaggaata agacagtggt ggaagctcaa acctggccca
     2041 ccaccaccaa agcccgcaga gcggcataag gacgacagca ggggtcttgt gcttcctggg
     2101 tacaagtacc tcggaccctt caacggactc gacaagggag agccggtcaa cgaggcagac
     2161 gccgcggccc tcgagcacga caaagcctac gaccggcagc tcgacagcgg agacaacccg
     2221 tacctcaagt acaaccacgc cgacgccgag ttccaggagc ggctcaaaga agatacgtct
     2281 tttgggggca acctcgggcg agcagtcttc caggccaaaa agaggcttct tgaacctctt
     2341 ggtctggttg aggaagcggc taagacggct cctggaaaga agaggcctgt agagcactct
     2401 cctgtggagc cagactcctc ctcgggaacc ggaaaggcgg gccagcagcc tgcaagaaaa
     2461 agattgaatt ttggtcagac tggagacgca gactcagtcc cagaccctca accaatcgga
     2521 gaacctcccg cagccccctc aggtgtggga tctcttacaa tggctgcagg cggtggcgca
     2581 ccaatggcag acaataacga gggcgccgac ggagtgggta attcctcggg aaattggcat
     2641 tgcgattcca catggatggg cgacagagtc atcaccacca gcacccgaac ctgggccctg
     2701 cccacctaca acaaccacct ctacaagcaa atctccaaca gcacatctgg aggatcttca
     2761 aatgacaacg cctacttcgg ctacagcacc ccctgggggt attttgactt taacagattc
     2821 cactgccact tttcaccacg tgactggcag cgactcatca acaacaactg gggattccgg
     2881 cccaagagac tcagcttcaa gctcttcaac atccaggtca aggaggtcac gcagaatgaa
     2941 ggcaccaaga ccatcgccaa taacctcacc agcaccatcc aggtgtttac ggactcggag
     3001 taccagctgc cgtacgttct cggctctgcc caccagggct gcctgcctcc gttcccggcg
     3061 gacgtgttca tgattcccca gtacggctac ctaacactca acaacggtag tcaggccgtg
     3121 ggacgctcct ccttctactg cctggaatac tttccttcgc agatgctgag aaccggcaac
     3181 aacttccagt ttacttacac cttcgaggac gtgcctttcc acagcagcta cgcccacagc
     3241 cagagcttgg accggctgat gaatcctctg attgaccagt acctgtacta cttgtctcgg
     3301 actcaaacaa caggaggcac gacaaatacg cagactctgg gcttcagcca aggtgggcct
     3361 aatacaatgg ccaatcaggc aaagaactgg ctgccaggac cctgttaccg ccagcagcga
     3421 gtatcaaaga catctgcgga taacaacaac agtgaatact cgtggactgg agctaccaag
     3481 taccacctca atggcagaga ctctctggtg aatccgggcc cggccatggc aagccacaag
     3541 gacgatgaag aaaagttttt tcctcagagc ggggttctca tctttgggaa gcaaggctca
     3601 gagaaaacaa atgtggacat tgaaaaggtc atgattacag acgaagagga aatcaggaca
     3661 accaatcccg tggctacgga gcagtatggt tctgtatcta ccaacctcca gagaggctag
     3721 agacaagcag ctaccgcaga tgtcaacaca caaggcgttc ttccaggcat ggtctggcag
     3781 gacagagatg tgtaccttca ggggcccatc tgggcaaaga ttccacacac ggacggacat
     3841 tttcacccct ctcccctcat gggtggattc ggacttaaac accctccgcc tcagatcctg
     3901 atcaagaaca cgcctgtacc tgcggatcct ccgaccacct tcaaccagtc aaagctgaac
     3961 tctttcatca cccagtattc tactggccaa gtcagcgtgg agatcgagtg ggagctgcag
     4021 aaggaaaaca gcaagcgctg gaaccccgag atccagtaca cctccaacta ctacaaatct
     4081 acaagtgtgg actttgctgt taatacagaa ggcgtgtact ctgaaccccg ccccattggc
     4141 acccgttacc tcacccgtaa tctgtaattg cctgttaatc aataaaccgg ttgattcgtt
     4201 tcagttgaac tttggtctct gcgaagggcg aattcgttta aacctgcagg actagaggtc
     4261 ctgtattaga ggtcacgtga gtgttttgcg acattttgcg acaccatgtg gtcacgctgg
     4321 gtatttaagc ccgagtgagc acgcagggtc tccattttga agcgggaggt ttgaacgcgc
     4381 agccgccaag ccgaattctg cagatatcca tcacactggc ggccgctcga ctagagcggc
     4441 cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg
     4501 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     4561 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     4621 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     4681 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     4741 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     4801 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     4861 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     4921 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     4981 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     5041 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     5101 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     5161 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     5221 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     5281 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     5341 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     5401 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5461 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5521 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     5581 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     5641 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     5701 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     5761 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     5821 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     5881 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     5941 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     6001 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     6061 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     6121 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     6181 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     6241 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     6301 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6361 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6421 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     6481 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     6541 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     6601 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     6661 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     6721 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     6781 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     6841 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     6901 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     6961 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     7021 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     7081 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
     7141 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     7201 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     7261 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
     7321 gccccccctc gat
//
