LOCUS Exported 9317 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral plasmids encoding human OCT4 (POU5f1) along with GFP linked via P2A.. ACCESSION . VERSION . KEYWORDS pLVH-EF1a-GFP-P2A-OCT4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9317) AUTHORS Shao Z, Zhang R, Khodadadi-Jamayran A, Chen B, Crowley MR, Festok MA, Crossman DK, Townes TM, Hu K TITLE The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis. JOURNAL Nat Commun. 2016 Mar 7;7:10869. doi: 10.1038/ncomms10869. PUBMED 26947130 REFERENCE 2 (bases 1 to 9317) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9317 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2028..2143 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" promoter 2197..2408 /label=EF-1-alpha core promoter /note="core promoter for human elongation factor EF-1-alpha" intron 2409..3347 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3304..3324 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" regulatory 3364..3373 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3370..4086 /codon_start=1 /product="Aequorea coerulescens GFP" /label=AcGFP1 /note="mammalian codon-optimized" /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF GFVTAAAITHGMDELYK" CDS 4114..4170 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" misc_feature 5285..5873 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5338..5358) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5756..5767) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6080..6713 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" primer_bind complement(6842..6858) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6842..6858) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6855..6877) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6866..6882 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6890..6920) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6935..6956 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7073..7090) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7244..7832) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7324..7343) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8003..8863) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8626..8645 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8864..8968) /gene="bla" /label=AmpR promoter polyA_signal 9016..9150 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9053..9072) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9107..9126 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgtgccc gtcagtgggc agagcgcaca tcgcccacag 2221 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg 2281 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag 2341 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca 2401 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc 2461 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc 2521 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg 2581 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc 2641 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg 2701 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta 2761 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg 2821 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc 2881 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc 2941 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag 3001 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa 3061 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc 3121 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg 3181 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag 3241 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca 3301 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgacccg 3361 gtcgccacca tggtgagcaa gggcgccgag ctgttcaccg gcatcgtgcc catcctgatc 3421 gagctgaatg gcgatgtgaa tggccacaag ttcagcgtga gcggcgaggg cgagggcgat 3481 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcctgtgccc 3541 tggcccaccc tggtgaccac cctgagctac ggcgtgcagt gcttctcacg ctaccccgat 3601 cacatgaagc agcacgactt cttcaagagc gccatgcctg agggctacat ccaggagcgc 3661 accatcttct tcgaggatga cggcaactac aagtcgcgcg ccgaggtgaa gttcgagggc 3721 gataccctgg tgaatcgcat cgagctgacc ggcaccgatt tcaaggagga tggcaacatc 3781 ctgggcaata agatggagta caactacaac gcccacaatg tgtacatcat gaccgacaag 3841 gccaagaatg gcatcaaggt gaacttcaag atccgccaca acatcgagga tggcagcgtg 3901 cagctggccg accactacca gcagaatacc cccatcggcg atggccctgt gctgctgccc 3961 gataaccact acctgtccac ccagagcgcc ctgtccaagg accccaacga gaagcgcgat 4021 cacatgatct acttcggctt cgtgaccgcc gccgccatca cccacggcat ggatgagctg 4081 tacaagtccg gactcagatc tcgaggatct ggagcaacaa acttctcact actcaaacaa 4141 gcaggtgacg tggaggagaa tcccggcccc atggcgggac acctggcttc ggatttcgcc 4201 ttctcgcccc ctccaggtgg tggaggtgat gggccagggg ggccggagcc gggctgggtt 4261 gatcctcgga cctggctaag cttccaaggc cctcctggag ggccaggaat cgggccgggg 4321 gttgggccag gctctgaggt gtgggggatt cccccatgcc ccccgccgta tgagttctgt 4381 ggggggatgg cgtactgtgg gccccaggtt ggagtggggc tagtgcccca aggcggcttg 4441 gagacctctc agcctgaggg tgaagcagga gtcggggtgg agagcaactc cgatggggcc 4501 tccccggagc cctgcaccgt cacccctggt gccgtgaagc tggagaagga gaagctggag 4561 caaaacccgg aggagtccca ggacatcaaa gctctgcaga aagaactcga gcaatttgcc 4621 aagctcctga agcagaagag gatcaccctg ggatatacac aggccgatgt ggggctcacc 4681 ctgggggttc tatttgggaa ggtattcagc caaacgacca tctgccgctt tgaggctctg 4741 cagcttagct tcaagaacat gtgtaagctg cggcccttgc tgcagaagtg ggtggaggaa 4801 gctgacaaca atgaaaatct tcaggagata tgcaaagcag aaaccctcgt gcaggcccga 4861 aagagaaagc gaaccagtat cgagaaccga gtgagaggca acctggagaa tttgttcctg 4921 cagtgcccga aacccacact gcagcagatc agccacatcg cccagcagct tgggctcgag 4981 aaggatgtgg tccgagtgtg gttctgtaac cggcgccaga agggcaagcg atcaagcagc 5041 gactatgcac aacgagagga ttttgaggct gctgggtctc ctttctcagg gggaccagtg 5101 tcctttcctc tggccccagg gccccatttt ggtaccccag gctatgggag ccctcacttc 5161 actgcactgt actcctcggt ccctttccct gagggggaag cctttccccc tgtctctgtc 5221 accactctgg gctctcccat gcattcaaac tgataaggat ccaccggatc taggcgtctg 5281 gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 5341 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 5401 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 5461 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 5521 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 5581 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 5641 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct 5701 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 5761 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 5821 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat 5881 taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct 5941 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc 6001 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 6061 ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt 6121 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg 6181 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca 6241 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg 6301 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca 6361 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct 6421 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg 6481 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct 6541 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 6601 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 6661 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 6721 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 6781 gaggccttga cattgctagc gttttaccgt cgacctctag ctagagcttg gcgtaatcat 6841 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 6901 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 6961 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 7021 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 7081 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 7141 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 7201 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 7261 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 7321 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 7381 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 7441 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 7501 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 7561 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 7621 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 7681 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 7741 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 7801 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 7861 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 7921 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 7981 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 8041 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 8101 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 8161 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 8221 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 8281 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 8341 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 8401 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 8461 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 8521 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 8581 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 8641 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 8701 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 8761 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 8821 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 8881 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 8941 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg 9001 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc 9061 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 9121 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat 9181 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc 9241 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt 9301 actacaaact tagtagt //