LOCUS Exported 12529 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of FLT1 G886V. ACCESSION . VERSION . KEYWORDS pHAGE-FLT1-G886V SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12529) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 12529) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12529 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(7466..7490) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 7543..7584 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 7605..8172 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 7622..8174 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(7766..7783) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 7993..8012 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 8186..8905 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 8186..8902 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(8231..8252) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(8492..8511) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 8839..8860 /label=EGFP-C /note="EGFP, forward primer" misc_feature 8919..9507 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8972..8992) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(9390..9401) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 9582..9815 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(9934..9952) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 10020..10124 /gene="bla" /label=AmpR promoter CDS 10125..10985 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(10343..10362) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 11156..11744 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 11645..11664 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11898..11915 /label=L4440 /note="L4440 vector, forward primer" primer_bind 12007..12026 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 12155..12176 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 12191..12221 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 12229..12245 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 12234..12256 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 12253..12269 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 12253..12269 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 12319..12338 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcaccatg gtcagctact gggacaccgg ggtcctgctg 3481 tgcgcgctgc tcagctgtct gcttctcaca ggatctagtt caggttcaaa attaaaagat 3541 cctgaactga gtttaaaagg cacccagcac atcatgcaag caggccagac actgcatctc 3601 caatgcaggg gggaagcagc ccataaatgg tctttgcctg aaatggtgag taaggaaagc 3661 gaaaggctga gcataactaa atctgcctgt ggaagaaatg gcaaacaatt ctgcagtact 3721 ttaaccttga acacagctca agcaaaccac actggcttct acagctgcaa atatctagct 3781 gtacctactt caaagaagaa ggaaacagaa tctgcaatct atatatttat tagtgataca 3841 ggtagacctt tcgtagagat gtacagtgaa atccccgaaa ttatacacat gactgaagga 3901 agggagctcg tcattccctg ccgggttacg tcacctaaca tcactgttac tttaaaaaag 3961 tttccacttg acactttgat ccctgatgga aaacgcataa tctgggacag tagaaagggc 4021 ttcatcatat caaatgcaac gtacaaagaa atagggcttc tgacctgtga agcaacagtc 4081 aatgggcatt tgtataagac aaactatctc acacatcgac aaaccaatac aatcatagat 4141 gtccaaataa gcacaccacg cccagtcaaa ttacttagag gccatactct tgtcctcaat 4201 tgtactgcta ccactccctt gaacacgaga gttcaaatga cctggagtta ccctgatgaa 4261 aaaaataaga gagcttccgt aaggcgacga attgaccaaa gcaattccca tgccaacata 4321 ttctacagtg ttcttactat tgacaaaatg cagaacaaag acaaaggact ttatacttgt 4381 cgtgtaagga gtggaccatc attcaaatct gttaacacct cagtgcatat atatgataaa 4441 gcattcatca ctgtgaaaca tcgaaaacag caggtgcttg aaaccgtagc tggcaagcgg 4501 tcttaccggc tctctatgaa agtgaaggca tttccctcgc cggaagttgt atggttaaaa 4561 gatgggttac ctgcgactga gaaatctgct cgctatttga ctcgtggcta ctcgttaatt 4621 atcaaggacg taactgaaga ggatgcaggg aattatacaa tcttgctgag cataaaacag 4681 tcaaatgtgt ttaaaaacct cactgccact ctaattgtca atgtgaaacc ccagatttac 4741 gaaaaggccg tgtcatcgtt tccagacccg gctctctacc cactgggcag cagacaaatc 4801 ctgacttgta ccgcatatgg tatccctcaa cctacaatca agtggttctg gcacccctgt 4861 aaccataatc attccgaagc aaggtgtgac ttttgttcca ataatgaaga gtcctttatc 4921 ctggatgctg acagcaacat gggaaacaga attgagagca tcactcagcg catggcaata 4981 atagaaggaa agaataagat ggctagcacc ttggttgtgg ctgactctag aatttctgga 5041 atctacattt gcatagcttc caataaagtt gggactgtgg gaagaaacat aagcttttat 5101 atcacagatg tgccaaatgg gtttcatgtt aacttggaaa aaatgccgac ggaaggagag 5161 gacctgaaac tgtcttgcac agttaacaag ttcttataca gagacgttac ttggatttta 5221 ctgcggacag ttaataacag aacaatgcac tacagtatta gcaagcaaaa aatggccatc 5281 actaaggagc actccatcac tcttaatctt accatcatga atgtttccct gcaagattca 5341 ggcacctatg cctgcagagc caggaatgta tacacagggg aagaaatcct ccagaagaaa 5401 gaaattacaa tcagagatca ggaagcacca tacctcctgc gaaacctcag tgatcacaca 5461 gtggccatca gcagttccac cactttagac tgtcatgcta atggtgtccc cgagcctcag 5521 atcacttggt ttaaaaacaa ccacaaaata caacaagagc ctggaattat tttaggacca 5581 ggaagcagca cgctgtttat tgaaagagtc acagaagagg atgaaggtgt ctatcactgc 5641 aaagccacca accagaaggg ctctgtggaa agttcagcat acctcactgt tcaaggaacc 5701 tcggacaagt ctaatctgga gctgatcact ctaacatgca cctgtgtggc tgcgactctc 5761 ttctggctcc tattaaccct ctttatccga aaaatgaaaa ggtcttcttc tgaaataaag 5821 actgactacc tatcaattat aatggaccca gatgaagttc ctttggatga gcagtgtgag 5881 cggctccctt atgatgccag caagtgggag tttgcccggg agagacttaa actgggcaaa 5941 tcacttggaa gaggggcttt tggaaaagtg gttcaagcat cagcatttgg cattaagaaa 6001 tcacctacgt gccggactgt ggctgtgaaa atgctgaaag agggggccac ggccagcgag 6061 tacaaagctc tgatgactga gctaaaaatc ttgacccaca ttgtccacca tctgaacgtg 6121 gttaacctgc tgggagcctg caccaagcaa ggagggcctc tgatggtgat tgttgaatac 6181 tgcaaatatg gaaatctctc caactacctc aagagcaaac gtgacttatt ttttctcaac 6241 aaggatgcag cactacacat ggagcctaag aaagaaaaaa tggagccagg cctggaacaa 6301 ggcaagaaac caagactaga tagcgtcacc agcagcgaaa gctttgcgag ctccggcttt 6361 caggaagata aaagtctgag tgatgttgag gaagaggagg attctgacgg tttctacaag 6421 gagcccatca ctatggaaga tctgatttct tacagttttc aagtggccag aggcatggag 6481 ttcctgtctt ccagaaagtg cattcatcgg gacctggcag cgagaaacat tcttttatct 6541 gagaacaacg tggtgaagat ttgtgatttt ggccttgccc gggatattta taagaacccc 6601 gattatgtga gaaaaggaga tactcgactt cctctgaaat ggatggctcc tgaatctatc 6661 tttgacaaaa tctacagcac caagagcgac gtgtggtctt acggagtatt gctgtgggaa 6721 atcttctcct taggtgggtc tccataccca ggagtacaaa tggatgagga cttttgcagt 6781 cgcctgaggg aaggcatgag gatgagagct cctgagtact ctactcctga aatctatcag 6841 atcatgctgg actgctggca cagagaccca aaagaaaggc caagatttgc agaacttgtg 6901 gaaaaactag gtgatttgct tcaagcaaat gtacaacagg atggtaaaga ctacatccca 6961 atcaatgcca tactgacagg aaatagtggg tttacatact caactcctgc cttctctgag 7021 gacttcttca aggaaagtat ttcagctccg aagtttaatt caggaagctc tgatgatgtc 7081 agatacgtaa atgctttcaa gttcatgagc ctggaaagaa tcaaaacctt tgaagaactt 7141 ttaccgaatg ccacctccat gtttgatgac taccagggcg acagcagcac tctgttggcc 7201 tctcccatgc tgaagcgctt cacctggact gacagcaaac ccaaggcctc gctcaagatt 7261 gacttgagag taaccagtaa aagtaaggag tcggggctgt ctgatgtcag caggcccagt 7321 ttctgccatt ccagctgtgg gcacgtcagc gaaggcaagc gcaggttcac ctacgaccac 7381 gctgagctgg aaaggaaaat cgcgtgctgc tccccgcccc cagactacaa ctcggtggtc 7441 ctgtactcca ccccacccat ctagaaccca gctttcttgt acaaagtggt tgatatccag 7501 cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc tatccctaac 7561 cctctcctcg gtctcgattc tacgcgtacc ggttagtaat gagatccctc ccccccccct 7621 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt 7681 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg 7741 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc 7801 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt 7861 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta 7921 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg 7981 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag 8041 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag 8101 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa 8161 acacgatgat aatatggcca cacatatggt gagcaagggc gaggagctgt tcaccggggt 8221 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg 8281 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg 8341 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt 8401 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg 8461 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga 8521 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa 8581 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta 8641 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat 8701 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg 8761 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc 8821 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct 8881 cggcatggac gagctgtaca agtaaatcga tagatcctaa tcaacctctg gattacaaaa 8941 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg 9001 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 9061 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg 9121 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct 9181 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 9241 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg 9301 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc 9361 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 9421 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc 9481 ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga cttacaaggc 9541 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc 9601 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg 9661 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 9721 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 9781 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 9841 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg ggttaattaa 9901 ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg 9961 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc 10021 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac 10081 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt 10141 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag 10201 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg 10261 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa 10321 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc 10381 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag 10441 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa 10501 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc 10561 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg 10621 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa 10681 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa 10741 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg 10801 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag 10861 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg 10921 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt 10981 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt 11041 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 11101 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 11161 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 11221 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 11281 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga 11341 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 11401 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 11461 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 11521 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 11581 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 11641 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 11701 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 11761 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 11821 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca 11881 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca 11941 aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg gctgactaat 12001 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg 12061 aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac gacaggtttc 12121 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg 12181 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat 12241 aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat aaagcatttt 12301 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga 12361 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta 12421 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt 12481 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt //