LOCUS Exported 3719 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBJ1-human b2m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3719) AUTHORS Olson R, Huey-Tubman KE, Dulac C, Bjorkman PJ TITLE Structure of a pheromone receptor-associated MHC molecule with an open and empty groove. JOURNAL PLoS Biol. 2005 Aug . 3(8):e257. PUBMED 16089503 REFERENCE 2 (bases 1 to 3719) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3719 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 40..58 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(128..148) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 133..746 /label=SR-alpha promoter /note="hybrid promoter consisting of the SV40 early promoter plus part of the long terminal repeat of human T-lymphotrophic virus 1" rep_origin 313..448 /label=SV40 ori /note="SV40 origin of replication" primer_bind 375..394 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" LTR 478..746 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" primer_bind complement(1163..1179) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1163..1179) /label=pBluescriptSK /note="For pBluescript vector" polyA_signal 1222..1356 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1246..1265) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 1300..1319 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(1499..1521) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 1662..1681 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(1796..1813) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1967..2555) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2047..2066) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2726..3586) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3349..3368 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3587..3691) /gene="bla" /label=AmpR promoter ORIGIN 1 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc 61 tttcgtcttc aagaattggt cgaccaattc tcatgtttga cagcttatca tcgataagct 121 tggctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga 181 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc 241 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc 301 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 361 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag 421 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctcct cgatcgaggg 481 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 541 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 601 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 661 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 721 ttttctgttc tgcgccgtta cagatccaag ctcctcgacc catgtctcgc tccgtggcct 781 tagctgtgct cgcgctactc tctctttctg gcctggaggc tatccagcgt actccaaaga 841 ttcaggttta ctcacgtcat ccagcagaga atggaaagtc aaatttcctg aattgctatg 901 tgtctgggtt tcatccatcc gacattgaag ttgacttact gaagaatgga gagagaattg 961 aaaaagtgga gcattcagac ttgtctttca gcaaggactg gtctttctat ctcttgtact 1021 acactgaatt cacccccact gaaaaagatg agtatgcctg ccgtgtgaac catgtgactt 1081 tgtcacagcc caagatagtt aagtgggatc gagacatgta agcagcatca taagcttgat 1141 atcgaattcc tgcagcccgg gggatccact agttctagag cggccgcgaa ttcgatatca 1201 agcttatcga taccgtcgag ggatccagac atgataagat acattgatga gtttggacaa 1261 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct 1321 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt 1381 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa 1441 tgtggtatgg ctgattatga tccggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc 1501 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca 1561 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc 1621 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt 1681 actgagagtg caccataggt cgaccggtcg accggtcgac cggtcgacct atgcggtgtg 1741 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc 1801 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 1861 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 1921 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 1981 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 2041 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 2101 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 2161 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 2221 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 2281 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 2341 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 2401 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 2461 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 2521 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 2581 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 2641 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 2701 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 2761 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 2821 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 2881 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 2941 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 3001 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 3061 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 3121 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 3181 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 3241 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 3301 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 3361 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 3421 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 3481 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 3541 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 3601 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 3661 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgac //