LOCUS       Exported               12039 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  HPHex_CrisprCas9-yl_RFP: CAS9 vector with Hygromycinex marker for 
            gRNA cloning using GoldenGate.
ACCESSION   .
VERSION     .
KEYWORDS    JME4580
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12039)
  AUTHORS   Larroude M, Trabelsi H, Nicaud JM, Rossignol T
  TITLE     A set of Yarrowia lipolytica CRISPR/Cas9 vectors for exploiting 
            wild-type strain diversity.
  JOURNAL   Biotechnol Lett. 2020 Jan 23. pii: 10.1007/s10529-020-02805-4. doi: 
            10.1007/s10529-020-02805-4.
  PUBMED    31974649
REFERENCE   2  (bases 1 to 12039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12039
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      12..69
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(93..110)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(264..852)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(344..363)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1023..1883)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1646..1665
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1884..1988)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      2078..2121
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     protein_bind    2700..2721
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2736..2766
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2774..2790
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2808..2819
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2826..3503
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      3548..3619
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3635..3662
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    4007..4040
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             4405..5430
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(5652..5674)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5774..5793
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5987..6009
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6001..6018
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6002..6018
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             7631..11734
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             11747..11767
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      11778..12025
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(11794..11812)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 tcttcctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa
       61 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc
      121 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      181 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      241 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
      301 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      361 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      421 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      481 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      541 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      601 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
      661 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
      721 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
      781 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
      841 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
      901 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
      961 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1021 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1081 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1141 cccagtgctg caatgatacc gcgcgaccca cgctcaccgg ctccagattt atcagcaata
     1201 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1261 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1321 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     1381 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     1441 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     1501 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     1561 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     1621 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     1681 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     1741 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     1801 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     1861 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     1921 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     1981 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     2041 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta
     2101 gctttcgcta aggatgattt ctggaattcg aagagcctct gacctgacgt ccccagttgc
     2161 aaaagttgac acaactctag atctgcttcc aaatatagaa tcataacaag ggttagggtg
     2221 tgattatata atattggtct taattgatgt gctagggctt taaaagttgg ttaaaataac
     2281 gctctaatgc ctttttaata tattgtcttt ttcaaaatct caaatcggac acttcttcgt
     2341 gtatgagact ccattttttg gctccgtcac gtgatatgta ttatcagcta tagtggtgta
     2401 aacaaagttt tttactagct gtaatggcat tttgtcggag tggtaaatcg ccttcttgtt
     2461 gtgcgttcga gttctggact ctgcactggg ctactttgaa aaatacctct aatgcgccga
     2521 tggtttagtg gtaaaatcca tcgttgccat cgatgggccc ccggttcgag agacggggcc
     2581 ggccatctgg gccctagagc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     2641 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     2701 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     2761 atgttgtgtg gaattgtgag cggataacaa tttcacacat actagagaaa gaggagaaat
     2821 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg
     2881 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg
     2941 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg
     3001 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca
     3061 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact
     3121 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca
     3181 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa
     3241 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag
     3301 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa
     3361 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac
     3421 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag
     3481 gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc
     3541 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     3601 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     3661 cgtttatata ggccatctgg gccgggggcg tctctgagct agaaatagca agttaaaata
     3721 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tttacgtcta
     3781 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggcccagatc
     3841 ctctagagtc gaagcggccg ctatgtctga taaaaggatg taacataggc aagctgctcg
     3901 tgagtgttga gtacgaacct tagatccaaa tcacccgcac ccacggatat acttgcttga
     3961 atatacagta gtatgcggcc gctaggttag ggataacagg gtaatgataa cttcgtatag
     4021 catacattat acgaagttat gggttggcgg cgcatttgtg tcccaaaaaa cagccccaat
     4081 tgccccaatt gaccccaaat tgacccagta gcggacccaa ccccggcgag agcccccttc
     4141 accccacata tcaaacctcc cccggttccc acacttgccg ttaagggcgt agggtactgc
     4201 agtctggaat ctacgcttgt tcagactttg tactagtttc tttgtctggc catccgggta
     4261 acccatgccg gacgcaaaat agactactga aaattttttt gctttgtggt tgggacttta
     4321 gccaagggta taaaagacca ccgtccccga attacctttc ctcttctttt ctctctctcc
     4381 ttgtcaactc acacccgaag cacaatgaag aagcccgagc tgaccgctac ctctgttgag
     4441 aagttcctga ttgagaagtt tgattccgtt tccgacctga tgcagctgtc cgagggcgag
     4501 gagtctcgag ccttctcctt tgacgtgggc ggacgaggtt acgttctgcg agtgaactcg
     4561 tgtgccgacg gcttctacaa ggatcgatac gtctaccgac actttgcttc tgccgctctg
     4621 cccatccctg aggttctcga cattggcgag ttctctgagt ccctcaccta ctgcatctct
     4681 cgacgagctc agggagtcac cctgcaggac ctccctgaga ctgagctgcc tgctgtcctc
     4741 cagcctgttg ctgaggccat ggacgctatc gctgctgctg atctgtccca gacctcgggt
     4801 ttcggcccct ttggacctca gggaattgga cagtacacca cttggcgaga cttcatctgt
     4861 gctattgccg atcctcacgt ctaccattgg cagaccgtta tggacgatac tgtgtcggct
     4921 tctgtcgctc aggctctgga cgagctgatg ctctgggccg aggattgccc cgaggttcga
     4981 cacctggtgc atgctgactt cggttccaac aacgttctca ccgacaacgg ccgaatcact
     5041 gccgtgattg actggtccga ggctatgttt ggcgactcgc agtacgaggt ggccaacatc
     5101 ttcttttggc gaccctggct ggcttgtatg gagcagcaga cccgatactt cgagcgacga
     5161 catcctgagc tcgctggctc ccctcgactg cgagcttaca tgctccgaat tggtctggac
     5221 cagctctacc agtcgctggt ggatggcaac tttgacgatg ctgcctgggc tcagggacga
     5281 tgtgacgcca tcgtgcgatc tggcgctgga accgtcggac gaactcagat tgcccgacga
     5341 tccgctgctg tctggaccga cggatgcgtg gaggtcctgg ctgattcggg taaccgacga
     5401 ccctctactc gacctcgagc taaggagtaa gtgtctgtgg tatctaagct atttatcact
     5461 ctttacaact tctacctcaa ctatctactt taataaatga atatcgttta ttctctatga
     5521 ttactgtata tgcgttcctc taagacaaat cgaatataac ttcgtatagc aggagttatc
     5581 cgaagcgagt agggataaca gggtaatacg gggccctttc gtcacgcgcg tttcggtgat
     5641 gacggtgaaa acctctgaca catgcagctc ccggtgacgg tcacagcttg tctgtaagcg
     5701 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     5761 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     5821 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg
     5881 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     5941 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     6001 tgtaaaacga cggccagtga attcgagctc ggtacccgct tttcgtagat aatggaatac
     6061 aaatggatat ccagagtata cacatggata gtatacactg acacgacaat tctgtatctc
     6121 tttatgttaa ctactgtgag gcgttaaata gagcttgata tataaaatgt tacatttcac
     6181 agtctgaact tttgcagatt acctaatttg gtaagatatt aattatgaac tgaaagttga
     6241 tggcatccct aaatttgatg aaagggggaa cccccgggtt cgaaggtacc aaggaagcat
     6301 gcggtacccg aattcgcttc gatacgcgta tcgatgatac gcgtccatgg tgaggtgtct
     6361 cacaagtgcc gtgcagtccc gcccccactt gcttctcttt gtgtgtagtg tacgtacatt
     6421 atcgagaggg ttgttcccgc ccacctcgat ccggcatgct gaggtgtctc acaagtgccg
     6481 tgcagtcccg cccccacttg cttctctttg tgtgtagtgt acgtacatta tcgagagggt
     6541 tgttcccgcc cacctcgatc cggcatgctg aggtgtctca caagtgccgt gcagtcccgc
     6601 ccccacttgc ttctctttgt gtgtagtgta cgtacattat cgagagggtt gttcccgccc
     6661 acctcgatcc ggcatgctga ggtgtctcac aagtgccgtg cagtcccgcc cccacttgct
     6721 tctctttgtg tgtagtgtac gtacattatc gagagggttg ttcccgccca cctcgatccg
     6781 gcatgctgag gtgtctcaca agtgccgtgc agtcccgccc ccacttgctt ctctttgtgt
     6841 gtagtgtacg tacattatcg agagggttgt tcccgcccac ctcgatccgg catgctgagg
     6901 tgtctcacaa gtgccgtgca gtcccgcccc cacttgcttc tctttgtgtg tagtgtacgt
     6961 acattatcga gagggttgtt cccgcccacc tcgatccggc atgctgaggt gtctcacaag
     7021 tgccgtgcag tcccgccccc acttgcttct ctttgtgtgt agtgtacgta cattatcgag
     7081 agggttgttc ccgcccacct cgatccgaca tgttgaggtg tctcacaagt gccgtgcagt
     7141 cccgccccca cttgcttctc tttgtgtgta gtgtacgtac attatcgaga gggttgttcc
     7201 cgcccacctc gatccggcat gcactgatca cgggcaaaag tgcttcgata gagagggggt
     7261 tggcggcgca tttgtgtccc aaaaaacagc cccaattgcc ccaattgacc ccaaattgac
     7321 ccagtagcgg acccaacccc ggcgagagcc cccttcaccc cacatatcaa acctcccccg
     7381 gttcccacac ttgccgttaa gggcgtaggg tactgcagtc tggaatctac gcttgttcag
     7441 actttgtact agtttctttg tctggccatc cgggtaaccc atgccggacg caaaatagac
     7501 tactgaaaat ttttttgctt tgtggttggg actttagcca agggtataaa agaccaccgt
     7561 ccccgaatta cctttcctct tcttttctct ctctccttgt caactcacac ccgaaggatc
     7621 cacaaccaca atggataaga aatactccat tggcctggac atcggaacca actccgtggg
     7681 ttgggccgtg atcaccgatg agtacaaggt gccctctaag aaattcaagg tcctgggcaa
     7741 caccgaccga cactccatca agaagaacct gatcggcgct ctgctcttcg actctggcga
     7801 gactgctgag gccacccgac tgaagcgaac cgctcgaaga cgatacaccc gaagaaagaa
     7861 ccgaatctgt tacctgcagg agatcttctc taacgagatg gccaaggtgg acgactcttt
     7921 cttccaccga ctggaggagt ctttcctggt ggaggaggac aagaagcacg agcgacaccc
     7981 catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca
     8041 cctgcgaaag aagctggtgg actctaccga caaggccgac ctgcgactga tctacctggc
     8101 cctggcccac atgatcaagt tccgaggcca cttcctgatc gagggcgacc tgaaccccga
     8161 caactctgac gtggacaagc tgttcatcca gctggtgcag acctacaacc agctcttcga
     8221 agagaacccc attaacgctt ctggcgtgga tgctaaggcc atcctgtctg cccgactgtc
     8281 taagtctcga cgactcgaga acctgattgc tcagctcccc ggagagaaga agaacggtct
     8341 gttcggaaac ctgattgctc tgtccctggg tctgacccct aacttcaagt ccaacttcga
     8401 tctggctgag gacgctaagc tgcagctgtc taaggacacc tacgacgatg acctggataa
     8461 cctgctcgcc cagattggcg accagtacgc cgacctgttc ctggccgcca agaacctgtc
     8521 tgacgccatc ctgctgtctg acatcctgcg agtgaacacc gagatcacca aggcccccct
     8581 gtctgcctcc atgattaagc gatacgatga gcaccaccag gatctgaccc tcctcaaggc
     8641 tctggtccga cagcagctgc ccgagaagta caaggagatt ttcttcgacc agtctaagaa
     8701 cggctacgcc ggctacatcg acggcggcgc ctctcaggag gagttctaca agttcattaa
     8761 gcccatcctg gagaagatgg acggaaccga ggaactgctc gtgaagctga accgagagga
     8821 cctcctgcga aagcagcgaa ccttcgacaa cggctctatc ccccaccaga tccacctggg
     8881 cgagctgcac gccatcctgc gacgacagga ggacttctac cccttcctga aggacaaccg
     8941 agagaagatc gagaagatcc tgaccttccg aatcccctac tacgtgggac ccctggcccg
     9001 aggaaactct cgattcgctt ggatgacccg aaagtctgag gagactatta ccccctggaa
     9061 cttcgaggag gtggtggata agggcgcctc tgctcagtct ttcatcgagc gaatgaccaa
     9121 cttcgacaag aacctcccca acgagaaggt cctgcccaag cactctctgc tctacgagta
     9181 cttcaccgtc tacaacgagc tcaccaaggt caagtacgtg accgagggaa tgcgaaagcc
     9241 cgctttcctg tctggagagc agaagaaggc tattgtggat ctgctcttca agactaaccg
     9301 aaaggtcacc gtcaagcagc tgaaggagga ttacttcaag aagattgagt gtttcgattc
     9361 tgtcgagatc tccggcgtcg aggaccgatt caacgcctct ctgggtacct accacgacct
     9421 gctgaagatt atcaaggaca aggatttcct ggataacgag gagaacgagg atattctcga
     9481 ggacattgtc ctgaccctca ccctgttcga ggatcgagag atgattgagg agcgactcaa
     9541 gacctacgct cacctgttcg acgacaaggt gatgaagcag ctgaagcgac gacgatacac
     9601 cggctggggc cgactgtctc gaaagctgat caacggcatc cgagacaagc agtctggcaa
     9661 gaccatcctg gacttcctga agtctgacgg cttcgccaac cgaaacttca tgcagctgat
     9721 ccacgacgac tctctgacct tcaaggagga catccagaag gcccaggtgt ctggccaggg
     9781 cgactctctg cacgagcaca tcgccaacct ggccggctct cccgccatta agaaaggtat
     9841 cctgcagacc gtcaaggtgg tcgatgagct cgtcaaggtg atgggccgac acaagcccga
     9901 gaacattgtc attgagatgg ctcgagagaa ccagactact cagaagggtc agaaaaactc
     9961 ccgagagcga atgaagcgaa ttgaggaagg tattaaggag ctgggctccc agattctcaa
    10021 ggagcatccc gtggagaaca ctcagctcca gaacgagaag ctgtacctgt actatctgca
    10081 gaacggtcga gacatgtacg tcgaccagga gctggatatc aaccgactct ccgactacga
    10141 tgtggaccac attgtgcccc agtccttcct gaaggacgat tctatcgata acaaggtgct
    10201 gacccgatcc gacaagaacc gaggcaagtc tgacaacgtg ccctccgagg aggtggtcaa
    10261 gaagatgaag aactactggc gacagctgct gaacgccaag ctgattaccc agcgaaagtt
    10321 cgacaacctg accaaggccg agcgaggcgg cctgtctgag ctggacaagg ccggcttcat
    10381 caagcgacag ctggtggaga ctcgacagat caccaagcac gtggcccaga tcctggactc
    10441 tcgaatgaac accaagtacg acgagaacga caagctgatc cgagaggtga aggtgatcac
    10501 cctgaagtct aagctggtgt ctgacttccg aaaggacttc cagttctaca aggtgcgaga
    10561 gattaacaac taccaccacg cccacgatgc ctacctgaac gctgtcgtgg gcaccgccct
    10621 catcaagaag tatcccaagc tggagtccga gttcgtctac ggcgactaca aggtctacga
    10681 tgtgcgaaaa atgattgcca agtccgagca ggagattggc aaggctaccg ccaagtactt
    10741 cttctactcc aacattatga acttcttcaa gaccgagatt accctggcta acggcgagat
    10801 tcgaaagcga cccctcattg agactaacgg agagactggt gagatcgtgt gggacaaggg
    10861 acgagacttc gccaccgtgc gaaaggtgct gtctatgccc caggtgaaca tcgtgaagaa
    10921 gaccgaggtg cagaccggag gtttctctaa ggagtccatc ctgcccaagc gaaactctga
    10981 caagctgatc gcccgaaaga aggactggga ccccaagaag tacggaggtt tcgactctcc
    11041 caccgtggct tactctgtgc tggtggtggc caaggtggag aagggcaagt ctaagaagct
    11101 gaagtctgtg aaggagctgc tgggcattac catcatggag cgatcttctt tcgagaagaa
    11161 ccccattgac ttcctggagg ccaagggata caaggaggtg aagaaagatc tgattatcaa
    11221 gctccccaag tactctctgt tcgagctgga gaacggacga aagcgaatgc tggcctctgc
    11281 cggcgagctg cagaagggaa acgagctggc cctgccctcc aagtacgtca acttcctgta
    11341 cctcgcctcc cattacgaga agctgaaggg ctctcccgag gataacgagc agaagcagct
    11401 cttcgtggag cagcataagc actacctgga cgagatcatc gagcagattt ctgagttctc
    11461 taagcgagtg atcctggctg acgccaacct ggataaggtg ctgtctgctt acaacaagca
    11521 ccgagacaag cccattcgag agcaggctga gaacatcatt cacctgttca ccctgaccaa
    11581 cctgggagcc cccgctgcct tcaagtactt cgacaccacc atcgaccgaa agcgatacac
    11641 ctctaccaag gaggtgctgg acgccaccct gatccaccag tctatcaccg gcctgtacga
    11701 gactcgaatc gacctgtctc agctgggcgg cgactctcga gccgacccca agaagaagcg
    11761 aaaggtgtaa gctagcctca tgtaattagt tatgtcacgc ttacattcac gccctccccc
    11821 cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta
    11881 tttttttata gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt
    11941 tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt
    12001 gggacgctcg aaggctttaa tttgcaagct tggcggtct
//
