LOCUS       Exported                 435 bp ds-DNA     linear   SYN 17-MAY-2021
DEFINITION  Expresses SpyCatcher003 in bacterial cytoplasm .
ACCESSION   .
VERSION     .
KEYWORDS    SpyCatcher003
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Keeble AH, Turkki P, Stokes S, Khairil Anuar INA, Rahikainen R, 
            Hytonen VP, Howarth M
  TITLE     Approaching infinite affinity through engineering of peptide-protein
            interaction.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 10. pii: 1909653116. doi: 
            10.1073/pnas.1909653116.
  PUBMED    31822621
REFERENCE   2  (bases 1 to 435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             52..72
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac gattacgaca tcccaacgac cgaaaacctg
       61 tattttcagg gcgccatggt aaccacctta tcaggtttat caggtgagca aggtccgtcc
      121 ggtgatatga caactgaaga agatagtgct acccatatta aattctcaaa acgtgatgag
      181 gacggccgtg agttagctgg tgcaactatg gagttgcgtg attcatctgg taaaactatt
      241 agtacatgga tttcagatgg acatgtgaag gatttctacc tgtatccagg aaaatataca
      301 tttgtcgaaa ccgcagcacc agacggttat gaggtagcaa ctccaattga atttacagtt
      361 aatgaggacg gtcaggttac tgtagatggt gaagcaactg aaggtgacgc tcatactgga
      421 tccagtggta gctaa
//
