LOCUS       Exported                 822 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  Expresses SpyTag003-superfolder GFP in bacterial cytoplasm.
ACCESSION   .
VERSION     .
KEYWORDS    SpyTag003-sfGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Keeble AH, Turkki P, Stokes S, Khairil Anuar INA, Rahikainen R, 
            Hytonen VP, Howarth M
  TITLE     Approaching infinite affinity through engineering of peptide-protein
            interaction.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 10. pii: 1909653116. doi: 
            10.1073/pnas.1909653116.
  PUBMED    31822621
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             73..783
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             802..819
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggacgtg gcgttcctca tattgttatg gtggacgcct acaaacgcta taaatcggga
       61 ggtggttcag gccgtaaagg cgaagagctg ttcactggtg tcgtccctat tctggtggaa
      121 ctggatggtg atgtcaacgg tcataagttt tccgtgcgtg gcgagggtga aggtgacgca
      181 actaatggta aactgacgct gaagttcatc tgtactactg gtaaactgcc ggtaccttgg
      241 ccgactctgg taacgacgct gacttatggt gttcagtgct ttgctcgtta tccggaccat
      301 atgaagcagc atgacttctt caagtccgcc atgccggaag gctatgtgca ggaacgcacg
      361 atttccttta aggatgacgg cacgtacaaa acgcgtgcgg aagtgaaatt tgaaggcgat
      421 accctggtaa accgcattga gctgaaaggc attgacttta aagaagacgg caatatcctg
      481 ggccataagc tggaatacaa ttttaacagc cacaatgttt acatcaccgc cgataaacaa
      541 aaaaatggca ttaaagcgaa ttttaaaatt cgccacaacg tggaggatgg cagcgtgcag
      601 ctggctgatc actaccagca aaacactcca atcggtgatg gtcctgttct gctgccagac
      661 aatcactatc tgagcacgca aagcgttctg tctaaagatc cgaacgagaa acgcgatcat
      721 atggttctgc tggagttcgt aaccgcagcg ggcatcacgc atggtatgga tgaactgtac
      781 aaaggctccg gctccggctc ccaccatcac catcaccatt ga
//
