LOCUS Exported 8639 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Fos upstream msfYFP 24xMS2 Fos downstream for CRISPR tagging. ACCESSION . VERSION . KEYWORDS pAAV Fos YFP MS2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8639) AUTHORS Wilson MZ, Ravindran PT, Lim WA, Toettcher JE TITLE Tracing Information Flow from Erk to Target Gene Induction Reveals Mechanisms of Dynamic and Combinatorial Control. JOURNAL Mol Cell. 2017 Sep 7;67(5):757-769.e5. doi: 10.1016/j.molcel.2017.07.016. Epub 2017 Aug 17. PUBMED 28826673 REFERENCE 2 (bases 1 to 8639) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8639 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 6147..6602 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6234..6253) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6444..6465 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(6619..6638) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 6738..6760 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(6798..6816) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 6884..6988 /gene="bla" /label=AmpR promoter CDS 6989..7849 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7207..7226) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8020..8608 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8509..8528 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tggccttttg ctggcctttt gctcacatgt ccaacaagtc tcgagcaaag agtcgctaac 61 tagagtttgg gaggcggcaa accgcggcaa tcccccctcc cggggcagcc tggagcaggg 121 aggagggagg agggaggagg gtgctgcggg cgggtgtgta aggcagtttc attgataaaa 181 agcgagttca ttctggagac tccggagcag cgcctgcgtc agcgcagacg tcagggatat 241 ttataacaaa ccccctttcg agcgagtgat gccgaaggga taacgggaac gcagcagtag 301 gatggaggag aaaggctgcg ctgcggaatt caagggagga tattgggaga gcttttatct 361 ccgatgaggt gcatacagga agacataagc agtctctgac cggaatgctt ctctctccct 421 gcttcatgcg acactagggc cacttgctcc acctgtgtct ggaacctcct cgctcacctc 481 cgctttcctc tttttgtttt gtttcaggac ttttgcgcag atctgtccgt ctctagtgcc 541 aactttatcc ccacggtgac agccatctcc accagcccag acctgcagtg gctggtgcag 601 cccactctgg tctcctccgt ggccccatcg cagaccagag cgccccatcc ttacggactc 661 cccacccagt ctgctggggc ttacgccaga gcgggaatgg tgaagaccgt gtcaggaggc 721 agagcgcaga gcatcggcag aaggggcaaa gtagagcagg tgagcagcga ttctggacct 781 ttgtgggctg gggggggggg ggggcggaga ctgacgcaca gaccacacaa cagagaaggg 841 acgctactga ctgcacttcc tgaccaggag ctgtggctgc tagccctttc cctcccttgt 901 cagattttga cagttggacc caagacaaac tctagacagt ttccctgaca gcttcctact 961 tcattctcta gccggggagc ttctttgttc ccctgctaaa gatctcactt taaatgcaaa 1021 tcacactctg cctgccaact gcaggttaga aaaactgctt caccgagagg tgcgggtgct 1081 gtaggagcca gtttcactgg ggtgactgaa tggaggtgac actagacaac cttaactgaa 1141 tgttggtcct tttcttctat agctatctcc tgaagaggaa gagaaacgga gaatccgaag 1201 ggaacggaat aagatggctg cagccaagtg ccggaatcgg aggagggagc tgacagatac 1261 actccaagcg gtaggttgaa ccagctgctg ctcctgaaac tttattaaag ttggagcttg 1321 ggactatggg cgcagggtcc ttgagcatgc ccgtgtctta tgctttctta tatctctccc 1381 tatgcaggag acagatcaac ttgaagatga gaagtctgcg ttgcagactg agattgccaa 1441 tctgctgaaa gagaaggaaa aactggagtt tattttggca gcccaccgac ctgcctgcaa 1501 gatccccgat gaccttggct tcccagagga gatgtctgtg gcctccctgg atttgactgg 1561 aggtctgcct gaggcttcca ccccagagtc tgaggaggcc ttcaccctgc cccttctcaa 1621 cgaccctgag cccaagccat ccttggagcc agtcaagagc atcagcaacg tggagctgaa 1681 ggcagaaccc tttgatgact tcttgtttcc ggcatcatct aggcccagtg gctcagagac 1741 ctcccgctct gtgccagatg tggacctgtc cggttccttc tatgcagcag actgggagcc 1801 tctgcacagc aattccttgg ggatggggcc catggtcaca gagctggagc ccctgtgtac 1861 tcccgtggtc acctgtactc cgggctgcac tacttacacg tcttcctttg tcttcaccta 1921 ccctgaagct gactccttcc caagctgtgc cgctgcccac cgaaagggca gcagcagcaa 1981 cgagccctcc tccgactccc tgagctcacc cacgctgctg gccctgggcg gatccggggg 2041 aagtaaaggt gaagaactgt tcaccggtgt tgttccgatc ctggttgaac tggatggtga 2101 tgttaacggc cacaaattct ctgttcgtgg tgaaggtgaa ggtgatgcaa ccaacggtaa 2161 actgaccctg aaattcatct gcactaccgg taaactgccg gttccatggc cgactctggt 2221 gactaccctg acctatggtg ttcagtgttt ttctcgttac ccggatcaca tgaagcagca 2281 tgatttcttc aaatctgcaa tgccggaagg ttatgtacag gagcgcacca tttctttcaa 2341 agacgatggc acctacaaaa cccgtgcaga ggttaaattt gaaggtgata ctctggtgaa 2401 ccgtattgaa ctgaaaggca ttgatttcaa agaggacggc aacatcctgg gccacaaact 2461 ggaatataac ttcaactccc ataacgttta catcaccgca gacaaacaga agaacggtat 2521 caaagctaac ttcaaaattc gccataacgt tgaagacggt agcgtacagc tggcggacca 2581 ctaccagcag aacactccga tcggtgatgg tccggttctg ctgccggata accactacct 2641 gtcctaccag tctaaactgt ccaaagaccc gaacgaaaag cgcgaccaca tggtgctgct 2701 ggagttcgtt actgcagcag gtatcacgca cggcatggat gaactctaca aatgataagc 2761 cgcgaattcg cccttcgcgg gatcctacgg tacttattgc caagaaagca cgagcatcag 2821 ccgtgcctcc aggtcgaatc ttcaaacgac gacgatcacg cgtcgctcca gtattccagg 2881 gttcatcaga tcctacggta cttattgcca agaaagcacg agcatcagcc gtgcctccag 2941 gtcgaatctt caaacgacga cgatcacgcg tcgctccagt attccagggt tcatcagatc 3001 ctacggtact tattgccaag aaagcacgag catcagccgt gcctccaggt cgaatcttca 3061 aacgacgacg atcacgcgtc gctccagtat tccagggttc atcagatcct acggtactta 3121 ttgccaagaa agcacgagca tcagccgtgc ctccaggtcg aatcttcaaa cgacgacgat 3181 cacgcgtcgc tccagtattc cagggttcat cagatcctac ggtacttatt gccaagaaag 3241 cacgagcatc agccgtgcct ccaggtcgaa tcttcaaacg acgacgatca cgcgtcgctc 3301 cagtattcca gggttcatca gatcctacgg tacttattgc caagaaagca cgagcatcag 3361 ccgtgcctcc aggtcgaatc ttcaaacgac gacgatcacg cgtcgctcca gtattccagg 3421 gttcatcaga tcctacggta cttattgcca agaaagcacg agcatcagcc gtgcctccag 3481 gtcgaatctt caaacgacga cgatcacgcg tcgctccagt attccagggt tcatcagatc 3541 ctacggtact tattgccaag aaagcacgag catcagccgt gcctccaggt cgaatcttca 3601 aacgacgacg atcacgcgtc gctccagtat tccagggttc atcagatcct acggtactta 3661 ttgccaagaa agcacgagca tcagccgtgc ctccaggtcg aatcttcaaa cgacgacgat 3721 cacgcgtcgc tccagtattc cagggttcat cagatcctac ggtacttatt gccaagaaag 3781 cacgagcatc agccgtgcct ccaggtcgaa tcttcaaacg acgacgatca cgcgtcgctc 3841 cagtattcca gggttcatca gatcctacgg tacttattgc caagaaagca cgagcatcag 3901 ccgtgcctcc aggtcgaatc ttcaaacgac gacgatcacg cgtcgctcca gtattccagg 3961 gttcatcaga tcctacggta cttattgcca agaaagcacg agcatcagcc gtgcctccag 4021 gtcgaatctt caaacgacga cgatcacgcg tcgctccagt attccagggt tcatcagatc 4081 taagggcgaa ttcgtttgca gtcagagaag gcaaggcagc cggcatccag acgtgccact 4141 gcccgagctg gtgcattaca gagaggagaa acacgtcttc cctcgaaggt tcccgtcgac 4201 ctagggagga ccttacctgt tcgtgaaaca caccaggctg tgggcctcaa ggacttgcaa 4261 gcatccacat ctggcctcca gtcctcacct cttccagaga tgtagcaaaa acaaaacaaa 4321 acaaaacaaa aaaccgcatg gagtgtgttg ttcctagtga cacctgagag ctggtagtta 4381 gtagagcatg tgagtcaagg cctggtctgt gtctcttttc tctttctcct tagttttctc 4441 atagcactaa ctaatctgtt gggttcatta ttggaattaa cctggtgctg gattgtatct 4501 agtgcagctg attttaacaa tacctactgt gttcctggca atagcgtgtt ccaattagaa 4561 acgaccaata ttaaactaag aaaagatagg actttatttt ccagtagata gaaatcaata 4621 gctatatcca tgtactgtag tccttcagcg tcaatgttca ttgtcatgtt actgatcatg 4681 cattgtcgag gtggtctgaa tgttctgaca ttaacagttt tccatgaaaa cgtttttatt 4741 gtgttttcaa tttatttatt aagatggatt ctcagatatt tatattttta ttttattttt 4801 ttctaccctg aggtctttcg acatgtggaa agtgaatttg aatgaaaaat tttaagcatt 4861 gtttgcttat tgttccaaga cattgtcaat aaaagcattt aagttgaatg cgaccacctt 4921 cttgctctct ttattctcag ttttgtatgg tttcaggaag gcctctgagg agaccagttt 4981 gtcaagatgg gtgggtcctg gaggggaaca cgccctctgt ccccttatca ctcagaggac 5041 acgctagttc agggtatttt acagagatgt gtgttcatat tgcattgcct gccttggggc 5101 tgaaggctgc ctgtaacagc ttgtgcaagg atgtgctttt ctgcatgctt atcttcgcct 5161 tagtgatttt catgggaaag gaatcctttg aacagagcct agctctacac aaactttttt 5221 gttgttgttg ttttgtgaac tgaagaagag ttgaaataag cgaccttcca cactgcaaac 5281 cagatcgcca cttccccctc ctgtccatta ttaggctgaa ctgcctcttt aaaaaaaaag 5341 tatatatata tatatatata tatatatata cacacacata tatatatata actaaaatat 5401 gtatttattt atgtggggag ggcattcatg tacttgaggt catgcacttg aggtcatggg 5461 acaacttcca gactttttct caagaacacg acctttggga attgaactca ggttagcagg 5521 cacctagacc caccaagcag ccaatcttgc cagtccccct ccccctttaa agatgtattt 5581 atttctattt tctgtgtaag ggtgtttcgc tggcatgtgt gtctatgcac caggtgcata 5641 tgcagtgtcc atagaggcca gaagagggca agggatcctg tgggactgga gttctgagac 5701 aatggcagct acccaagcaa gcaccaggaa tctaaatctg ggctctctgc aagtctaaca 5761 actgctttta actgctcatc cgtcttccta gccatcctga actaccttcc acaccttagc 5821 cttaaaaggt ttcccttggt taaaacacac acacacacac acacacacac acacacacac 5881 acacacgagt gcgcgcacat gcacgcaaac aaattttcaa ttgtttgttt acttttaaga 5941 tgggtctaac ttagcctagt ctgacctcaa acttgctatg tagctgaggc tggccctgta 6001 ttcctgatct ttctgtttat atctcccaag tgctggcttt acaggtatgc accgtcattc 6061 ggttccttac tcggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac 6121 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg 6181 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt 6241 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 6301 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 6361 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 6421 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc 6481 tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct attggttaaa 6541 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat 6601 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca 6661 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag 6721 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa 6781 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat 6841 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 6901 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 6961 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 7021 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 7081 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 7141 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 7201 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg 7261 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 7321 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 7381 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 7441 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 7501 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 7561 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 7621 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 7681 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 7741 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 7801 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 7861 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 7921 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 7981 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 8041 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 8101 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 8161 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 8221 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 8281 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 8341 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 8401 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 8461 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 8521 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 8581 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcc //