LOCUS       Exported               14615 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  ORFeus expression, mutant.
ACCESSION   .
VERSION     .
KEYWORDS    pDA027
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14615)
  AUTHORS   Ardeljan D, Steranka JP, Liu C, Li Z, Taylor MS, Payer LM, Gorbounov
            M, Sarnecki JS, Deshpande V, Hruban RH, Boeke JD, Fenyo D, Wu PH, 
            Smogorzewska A, Holland AJ, Burns KH
  TITLE     Cell fitness screens reveal a conflict between LINE-1 
            retrotransposition and DNA replication.
  JOURNAL   Nat Struct Mol Biol. 2020 Feb;27(2):168-178. doi: 
            10.1038/s41594-020-0372-1. Epub 2020 Feb 10.
  PUBMED    32042151
REFERENCE   2  (bases 1 to 14615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14615
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..315
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    45..63
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    80..98
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    116..134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    187..205
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    223..241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     288..312
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      344..353
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             5279..5302
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             5303..5368
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    5578..5712
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5855..5877)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6437..8226
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(10387..10405)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10473..10577
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10578..11438
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10796..10815)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11609..12197
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12098..12117
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12351..12368
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(12449..12468)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12487..12507)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12492..12821
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12672..12807
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12734..12753
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             12914..13513
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(12914..12933)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     13410..13430
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    13743..13877
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13942..13961)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    13986..14034
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
       61 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
      121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      181 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
      241 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
      301 aaccgtcaga tcgcctggag cggccgcgtt taaacttaat taagccacca tgggcaagaa
      361 gcagaaccgc aagaccggca acagcaagac ccagagcgcc agcccccccc ccaaggagcg
      421 cagcagcagc cccgccaccg agcagagctg gatggagaac gacttcgacg agctgcgcga
      481 ggagggcttc cgccgcagca actacagcga gctgcgcgag gacatccaga ccaagggcaa
      541 ggaggtggag aacttcgaga agaacctgga ggagtgcatc acccgcatca ccaacaccga
      601 gaagtgcctg aaggagctga tggagctgaa gaccaaggcc cgcgagctgc gcgaggagtg
      661 ccgcagcctg cgcagccgct gcgaccaatt ggaggagcgc gtgagcgcca tggaggacga
      721 gatgaacgag atgaagcgcg agggcaagtt ccgcgagaag cgcatcaagc gcaacgagca
      781 gagcctgcag gagatctggg actacgtgaa gcgccccaac ctgcgcctga tcggcgtgcc
      841 cgagagcgac gtggagaacg gcaccaagct ggagaacacc ctgcaggaca tcatccagga
      901 gaacttcccc aacctggccc gccaggccaa cgtgcagatc caggagatcc agcgcacccc
      961 ccagcgctac agcagccgcc gcgccacccc ccgccacatc atcgtgcgct tcaccaaggt
     1021 ggagatgaag gagaagatgc tgcgcgccgc ccgcgagaag ggccgcgtga ccctgaaggg
     1081 caagcccatc cgtctcaccg ccgacctgag cgccgagacc ctgcaggccc gccgcgagtg
     1141 gggccccatc ttcaacatcc tgaaggagaa gaacttccag ccccgcatca gctaccccgc
     1201 caagctgagc ttcatcagcg agggcgagat caagtacttc atcgacaagc agatgctgcg
     1261 cgacttcgtg accacccgcc ccgccctgaa ggagctgctg aaggaggccc tgaacatgga
     1321 gcgcaacaac cgctaccagc ccctgcagaa ccacgccaag atgtaaagac catcgagact
     1381 aggaagaaac tgcatcaact aatgagcaaa atcaggcgcg cccatcataa tgaccggcag
     1441 caacagccac atcaccatcc tgaccctgaa catcaacggc ctgaacagcg ccatcaagcg
     1501 ccaccgcctg gccagctgga tcaagagcca ggaccccagc gtgtgctgca tccaggagac
     1561 ccacctgacc tgccgcgaca cccaccgcct gaagatcaag ggctggcgca agatctacca
     1621 ggccaacggc aagcagaaga aggccggcgt ggccatcctg gtgagcgaca agaccgactt
     1681 caagcccacc aagatcaagc gcgacaagga gggccactac atcatggtga agggcagcat
     1741 ccagcaggag gagctgacca tcctgaacat ctacgccccc aacaccggtg ccccccgctt
     1801 catcaagcag gtgctgagcg acctgcagcg cgacctggac agccacaccc tgatcatggg
     1861 cgacttcaac acccccctga gcaccctgga ccgcagcacc cgccagaagg tgaacaagga
     1921 cacccaggag ctgaacagcg ccctgcacca ggccgacctg atcgacatct accgcaccct
     1981 gcaccccaag agcaccgagt acaccttctt cagcgccccc caccacacct acagcaagat
     2041 cgaccacatc gtgggcagca aggccctgct gagcaagtgc aagcgcaccg agatcatcac
     2101 caactacctg agcgacgcta gcgccatcaa gctggagctg cgcatcaaga acctgaccca
     2161 gagccgcagc accacctgga agctgaacaa cctgctgctg aacgactact gggtgcacaa
     2221 cgagatgaag gccgagatca agatgttctt cgaaaccaac gagaacaagg acaccaccta
     2281 ccagaacctg tgggacgcct tcaaggccgt gtgccgcggc aagttcatcg ccctgaacgc
     2341 ctacaagcgc aagcaggagc gcagcaagat cgacaccctg accagccagc tgaaggagct
     2401 ggagaagcag gagcagaccc acagcaaggc cagccgccgc caggagatca ccaagatccg
     2461 cgccgagctg aaggagatcg agacccagaa gaccctgcag aagatcaacg agtcgcgaag
     2521 ctggttcttc gagcgcatca acaagatcga ccgccccctg gcccgcctga tcaagaagaa
     2581 gcgcgagaag aaccagatcg acaccatcaa gaacgacaag ggcgacatca ccaccgaccc
     2641 caccgagatc cagaccacca tccgcgagta ctacaagcac ctgtacgcca acaagctgga
     2701 gaacctggag gagatggaca ccttcctgga cacctacacc ctgccccgcc tgaaccagga
     2761 ggaggtggag agcctgaacc gccccatcac cggcagcgag atcgtggcca tcatcaacag
     2821 cctgcccacc aagaagagcc ccggccccga cggcttcacc gccgagttct accagcgcta
     2881 caaggaggag ctggtgccct tcctgctgaa gctgttccaa agcatcgaga aggagggcat
     2941 cctgcccaac agcttctacg aggccagcat catcctgatc cccaagcccg gccgcgacac
     3001 caccaagaag gagaacttcc gccccatcag cctgatgaac atcgacgcca agatcctgaa
     3061 caagatcctg gccaaccgca tccagcagca catcaagaag ctgatccacc acgaccaggt
     3121 gggcttcatc cccgggatgc agggctggtt caacatccgc aagagcatca acgtgatcca
     3181 gcacatcaac cgcgccaagg acaagaacca catgatcatc agcatcgacg ccgagaaggc
     3241 cttcgacaag atccagcagc ccttcatgct gaagaccctg aacaagctgg gcatcgacgg
     3301 cacctacttc aagatcatcc gcgccatcta cgacaagccc accgccaaca tcatcctgaa
     3361 cggccagaag ctggaggcct tccccctgaa gaccggcacg cgtcagggct gccccctgag
     3421 ccccctgctg ttcaacatcg tgctggaggt gctggcccgc gccatccgcc aggagaagga
     3481 gatcaagggc atccagctgg gcaaggagga ggtgaagctg agcctgttcg cctacgacat
     3541 gatcgtgtac ctggagaacc ccatcgtgag cgcccagaac ctgctgaagc tgatcagcaa
     3601 cttcagcaag gtgagcggct acaagatcaa cgtgcagaag agccaggcct tcctgtacac
     3661 caacaaccgc cagaccgaga gccagatcat gggcgagctg cccttcacca tcgctagcaa
     3721 gcgcatcaag tacctgggca tccagctgac ccgcgacgtg aaggacctgt tcaaggagaa
     3781 ctacaagccc ctgctgaagg agatcaagga ggagaccaac aagtggaaga acatcccctg
     3841 cagctgggtg ggccgcatca acatcgtgaa gatggccatc ctgcccaagg tgatctaccg
     3901 cttcaacgcc atccccatca agctgcccat gaccttcttc accgagctgg agaagaccac
     3961 cctgaagttc atctggaacc agaagcgcgc ccgcatcgcc aagagcatcc tgagccagaa
     4021 gaacaaggcc ggcggcatca ccctgcccga cttcaagctg tactacaagg ccaccgtgac
     4081 caagaccgcc tggtactggt accagaaccg cgatatcgac cagtggaacc gcaccgagcc
     4141 cagcgagatc atgccccaca tctacaacta cctgatcttc gacaagcccg agaagaacaa
     4201 gcagtggggc aaggacagcc tgttcaacaa gtggtgctgg gagaactggc tggccatctg
     4261 ccgcaagctg aagctggacc ccttcctgac cccctacacc aagatcaaca gccgctggat
     4321 caaggacctg aacgtgaagc ccaagaccat caagaccctg gaggagaacc tgggcatcac
     4381 catccaggac atcggcgtgg gcaaggactt catgagcaag acccccaagg ccatggccac
     4441 caaggacaag atcgacaagt gggacctgat caagctgaag agcttctgca ccgccaagga
     4501 gaccaccatc cgcgtgaacc gccagcccac cacctgggag aagatcttcg ccacctacag
     4561 cagcgacaag ggcctgatca gccgcatcta caacgagctg aagcagatct acaagaagaa
     4621 gaccaacaac cccatcaaga agtgggccaa ggacatgaac cgccacttca gcaaggagga
     4681 catctacgcc gccaagaagc atatgaagaa gtgcagcagc agcctggcca tccgcgagat
     4741 gcagatcaag accaccatgc gctaccacct gacccccgtg cgcatggcca tcatcaagaa
     4801 gagcggcaac aaccgctgct ggcgcggctg cggcgagatc ggcaccctgc tgcactgctg
     4861 gtgggactgc aagctggtgc agcccctgtg gaagagcgtg tggcgcttcc tgcgcgacct
     4921 ggagctggag atccccttcg accccgccat ccccctgctg ggcatctacc ccaacgagta
     4981 caagagctgc tgctacaagg acacctgcac ccgcatgttc atcgccgccc tgttcaccat
     5041 cgccaagacc tggaaccagc ccaagtgccc caccatgatc gattggatca agaagatgtg
     5101 gcacatctac accatggagt actacgccgc catcaagaac gacgagttca tcagcttcgt
     5161 gggcacctgg atgaagctgg agaccatcat cctgagcaag ctgagccagg agcagaagac
     5221 caagcaccgc atcttcagcc tgatcggcgg caacggcggc ggcagcggcg gcggcagcct
     5281 ggaagttctg ttccaggggc ccgactacaa agaccatgac ggtgattata aagatcatga
     5341 catcgattac aaggatgacg atgacaagta gacaatgaga tcacatggac acaggaaggg
     5401 gaatatcaca ctctggggac tgtggtgggg tcgggggagg ggggagggat agcattggga
     5461 gatataccta atgctagatg acacattagt gggtgcagcg caccagcatg gcacatgtat
     5521 acatatgtaa ctaacctgca caatgtgcac atgtacccta aaacttagag tataatggat
     5581 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     5641 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     5701 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     5761 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatccg
     5821 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     5881 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     5941 cgggtgttgg cgggtgtcgg ggcgcagcca tgaggtcgac tctagaggat cgatccccgc
     6001 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
     6061 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
     6121 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
     6181 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
     6241 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
     6301 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
     6361 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
     6421 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
     6481 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
     6541 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
     6601 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
     6661 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     6721 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     6781 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     6841 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     6901 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     6961 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     7021 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     7081 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     7141 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     7201 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     7261 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     7321 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     7381 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     7441 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     7501 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     7561 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     7621 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     7681 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     7741 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     7801 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     7861 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     7921 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     7981 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     8041 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     8101 gcatatgcta tcctcatgca tatacagtca gcatatgata cccagtagta gagtgggagt
     8161 gctatccttt gcatatgccg ccacctccca agggggcgtg aattttcgct gcttgtcctt
     8221 ttcctgctgg ttgctcccat tcttaggtga atttaaggag gccaggctaa agccgtcgca
     8281 tgtctgattg ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc
     8341 gcggagctga gtgacgtgac aacatgggta tgcccaattg ccccatgttg ggaggacgaa
     8401 aatggtgaca agacagatgg ccagaaatac accaacagca cgcatgatgt ctactgggga
     8461 tttattcttt agtgcggggg aatacacggc ttttaatacg attgagggcg tctcctaaca
     8521 agttacatca ctcctgccct tcctcaccct catctccatc acctccttca tctccgtcat
     8581 ctccgtcatc accctccgcg gcagcccctt ccaccatagg tggaaaccag ggaggcaaat
     8641 ctactccatc gtcaaagctg cacacagtca ccctgatatt gcaggtagga gcgggctttg
     8701 tcataacaag gtccttaatc gcatccttca aaacctcagc aaatatatga gtttgtaaaa
     8761 agaccatgaa ataacagaca atggactccc ttagcgggcc aggttgtggg ccgggtccag
     8821 gggccattcc aaaggggaga cgactcaatg gtgtaagacg acattgtgga atagcaaggg
     8881 cagttcctcg ccttaggttg taaagggagg tcttactacc tccatatacg aacacaccgg
     8941 cgacccaagt tccttcgtcg gtagtccttt ctacgtgact cctagccagg agagctctta
     9001 aaccttctgc aatgttctca aatttcgggt tggaacctcc ttgaccacga tgctttccaa
     9061 accaccctcc ttttttgcgc ctgcctccat caccctgacc ccggggtcca gtgcttgggc
     9121 cttctcctgg gtcatctgcg gggccctgct ctatcgctcc cgggggcacg tcaggctcac
     9181 catctgggcc accttcttgg tggtattcaa aataatcggc ttcccctaca gggtggaaaa
     9241 atggccttct acctggaggg ggcctgcgcg gtggagaccc ggatgatgat gactgactac
     9301 tgggactcct gggcctcttt tctccacgtc cacgacctct ccccctggct ctttcacgac
     9361 ttccccccct ggctctttca cgtcctctac cccggcggcc tccactacct cctcgacccc
     9421 ggcctccact acctcctcga ccccggcctc cactgcctcc tcgaccccgg cctccacctc
     9481 ctgctcctgc ccctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg
     9541 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct gcccctcctg
     9601 cccctcctgc ccctcctcct gctcctgccc ctcctscygc tcctgcccct cctgctcctg
     9661 cccctcccgc tcctgctcct gctcctgttc caccgtgggt ccctttgcag ccaatgcaac
     9721 ttggacgttt ttggggtctc cggacaccat ctctatgtct tggccctgat cctgagccgc
     9781 ccggggctcc tggtcttccg cctcctcgtc ctcgtcctct tccccgtcct cgtccatggt
     9841 tatcaccccc tcttctttga ggtccactgc cgccggagcc ttctggtcca gatgtgtctc
     9901 ccttctctcc taggccattt ccaggtcctg tacctggccc ctcgtcagac atgattcaca
     9961 ctaaaagaga tcaatagaca tctttattag acgacgctca gtgaatacag ggagtgcaga
    10021 ctcctgcccc ctccaacagc ccccccaccc tcatcccctt catggtcgct gtcagacaga
    10081 tccaggtctg aaaattcccc atcctccgaa ccatcctcgt cctcatcacc aattactcgc
    10141 agcccggaaa actcccgctg aacatcctca agatttgcgt cctgagcctc aagccaggcc
    10201 tcaaattcct cgtccccctt tttgctggac ggtagggatg gggattctcg ggacccctcc
    10261 tcttcctctt caaggtcacc agacagagat gctactgggg caacggaaga aaagctgggt
    10321 gcggcctgtg aggatcagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac
    10381 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
    10441 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
    10501 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
    10561 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
    10621 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
    10681 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
    10741 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
    10801 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
    10861 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
    10921 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
    10981 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
    11041 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
    11101 agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg
    11161 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    11221 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    11281 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    11341 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    11401 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    11461 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    11521 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    11581 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    11641 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    11701 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
    11761 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    11821 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    11881 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    11941 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    12001 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    12061 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    12121 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    12181 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    12241 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    12301 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    12361 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
    12421 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
    12481 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
    12541 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
    12601 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
    12661 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
    12721 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
    12781 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgca tgcctgcagg
    12841 tcggccgcca cgaccggtgc cgccaccatc ccctgaccca cgcccctgac ccctcacaag
    12901 gagacgacct tccatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
    12961 cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
    13021 cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
    13081 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
    13141 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
    13201 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
    13261 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
    13321 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
    13381 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
    13441 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
    13501 gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc
    13561 ccatggctcc gaccgaagcc acccggggcg gccccgccga ccccgcaccc gcccccgagg
    13621 cccaccgact ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
    13681 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
    13741 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    13801 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    13861 cttatcatgt ctggatcact cgccgatagt ggaaaccgac gccccagcac tcgtccggat
    13921 cgggagatgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
    13981 tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac
    14041 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
    14101 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
    14161 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc cactggcccc gtgggttagg
    14221 gacggggtcc cccatgggga atggtttatg gttcgtgggg gttattattt tgggcgttgc
    14281 gtggggtcag gtccacgact ggactgagca gacagaccca tggtttttgg atggcctggg
    14341 catggaccgc atgtactggc gcgacacgaa caccgggcgt ctgtggctgc caaacacccc
    14401 cgacccccaa aaaccaccgc gcggatttct ggcgtgccaa gctagtcgac caattctcat
    14461 gtttgacagc ttatcatcgc agatccgggc aacgttgttg ccattgctgc aggcgcagaa
    14521 ctggtaggta tggaagatct ctagaagctg ggtaccagct gctagcaagc ttgctagcgg
    14581 ccgaggcgta tcacgaggcc ctttcgtctt cactc
//
