LOCUS Exported 5778 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Part Plasmid for dCas9, Part 3(coding sequence). ACCESSION . VERSION . KEYWORDS pMM586 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5778) AUTHORS Geller SH, Antwi EB, Di Ventura B, McClean MN TITLE Optogenetic Repressors of Gene Expression in Yeasts Using Light-Controlled Nuclear Localization. JOURNAL Cell Mol Bioeng. 2019 Sep 24;12(5):511-528. doi: 10.1007/s12195-019-00598-9. eCollection 2019 Oct. PUBMED 31719930 REFERENCE 2 (bases 1 to 5778) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5778 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 4143..4177 /label=lambda t0 terminator /note="minimal transcription terminator from phage lambda (Scholtissek and Grosse, 1987)" CDS complement(4248..4907) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 4822..4841 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" rep_origin 5089..5677 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5578..5597 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tcggtctcat atggacaaga agtattctat cggactggcc atcgggacta atagcgtcgg 61 gtgggccgtg atcactgacg agtacaaggt gccctctaag aagttcaagg tgctcgggaa 121 caccgaccgg cattccatca agaaaaatct gatcggagct ctcctctttg attcagggga 181 aaccgctgaa gcaacccgcc tcaagcggac tgctagacgg cggtacacca ggaggaagaa 241 ccggatttgt taccttcaag agatattctc caacgaaatg gcaaaggtcg acgacagctt 301 cttccatagg ctggaagaat cattcctcgt ggaagaggat aagaagcatg aacggcatcc 361 catcttcggt aatatcgtcg acgaggtggc ctatcacgag aaatacccaa ccatctacca 421 tcttcgcaaa aagctggtgg actcaaccga caaggcagac ctccggctta tctacctggc 481 cctggcccac atgatcaagt tcagaggcca cttcctgatc gagggcgacc tcaatcctga 541 caatagcgat gtggataaac tgttcatcca gctggtgcag acttacaacc agctctttga 601 agagaacccc atcaatgcaa gcggagtcga tgccaaggcc attctgtcag cccggctgtc 661 aaagagccgc agacttgaga atcttatcgc tcagctgccg ggtgaaaaga aaaatggact 721 gttcgggaac ctgattgctc tttcacttgg gctgactccc aatttcaagt ctaatttcga 781 cctggcagag gatgccaagc tgcaactgtc caaggacacc tatgatgacg atctcgacaa 841 cctcctggcc cagatcggtg accaatacgc cgaccttttc cttgctgcta agaatctttc 901 tgacgccatc ctgctgtctg acattctccg cgtgaacact gaaatcacca aggcccctct 961 ttcagcttca atgattaagc ggtatgatga gcaccaccag gacctgaccc tgcttaaggc 1021 actcgtccgg cagcagcttc cggagaagta caaggaaatc ttctttgacc agtcaaagaa 1081 tggatacgcc ggctacatcg acggaggtgc ctcccaagag gaattttata agtttatcaa 1141 acctatcctt gagaagatgg acggcaccga agagctcctc gtgaaactga atcgggagga 1201 tctgctgcgg aagcagcgca ctttcgacaa tgggagcatt ccccaccaga tccatcttgg 1261 ggagcttcac gccatccttc ggcgccaaga ggacttctac ccctttctta aggacaacag 1321 ggagaagatt gagaaaattc tcactttccg catcccctac tacgtgggac ccctcgccag 1381 aggaaatagc cggtttgctt ggatgaccag aaagtcagaa gaaactatca ctccctggaa 1441 cttcgaagag gtggtggaca agggagccag cgctcagtca ttcatcgaac ggatgactaa 1501 cttcgataag aacctcccca atgagaaggt cctgccgaaa cattccctgc tctacgagta 1561 ctttaccgtg tacaacgagc tgaccaaggt gaaatatgtc accgaaggga tgaggaagcc 1621 cgcattcctg tcaggcgaac aaaagaaggc aattgtggac cttctgttca agaccaatag 1681 aaaggtgacc gtgaagcagc tgaaggagga ctatttcaag aaaattgaat gcttcgactc 1741 tgtggagatt agcggggtcg aagatcggtt caacgcaagc ctgggtacct accatgatct 1801 gcttaagatc atcaaggaca aggattttct ggacaatgag gagaaagagg acatccttga 1861 ggacattgtc ctgactctca ctctgttcga ggaccgggaa atgatcgagg agaggcttaa 1921 gacctacgcc catctgttcg acgataaagt gatgaagcaa cttaaacgga gaagatatac 1981 cggatgggga cgccttagcc gcaaactcat caacggaatc cgggacaaac agagcggaaa 2041 gaccattctt gatttcctta agagcgacgg attcgctaat cgcaacttca tgcaacttat 2101 ccatgatgat tccctgacct ttaaggagga catccagaag gcccaagtgt ctggacaagg 2161 tgactcactg cacgagcata tcgcaaatct ggctggttca cccgctatta agaagggtat 2221 tctccagacc gtgaaagtcg tggacgagct ggtcaaggtg atgggtcgcc ataaaccaga 2281 gaacattgtc atcgagatgg ccagggaaaa ccagactacc cagaagggac agaagaacag 2341 cagggagcgg atgaaaagaa ttgaggaagg gattaaggag ctcgggtcac agatccttaa 2401 agagcacccg gtggaaaaca cccagcttca gaatgagaag ctctatctgt actaccttca 2461 aaatggacgc gatatgtatg tggaccaaga gcttgatatc aacaggctct cagactacga 2521 cgtggacgcc atcgtccctc agagcttcct caaagacgac tcaattgaca ataaggtgct 2581 gactcgctca gacaagaacc ggggaaagtc agataacgtg ccctcagagg aagtcgtgaa 2641 aaagatgaag aactattggc gccagcttct gaacgcaaag ctgatcactc agcggaagtt 2701 cgacaatctc actaaggctg agaggggcgg actgagcgaa ctggacaaag caggattcat 2761 taaacggcaa cttgtggaga ctcggcagat tactaaacat gtagcccaaa tccttgactc 2821 acgcatgaat accaagtacg acgaaaacga caaacttatc cgcgaggtga aggtgattac 2881 cctgaagtcc aagctggtca gcgatttcag aaaggacttt caattctaca aagtgcggga 2941 gatcaataac tatcatcatg ctcatgacgc atatctgaat gccgtggtgg gaaccgccct 3001 gatcaagaag tacccaaagc tggaaagcga gttcgtgtac ggagactaca aggtctacga 3061 cgtgcgcaag atgattgcca aatctgagca ggagatcgga aaggccaccg caaagtactt 3121 cttctacagc aacatcatga atttcttcaa gaccgaaatc acccttgcaa acggtgagat 3181 ccggaagagg ccgctcatcg agactaatgg ggagactggc gaaatcgtgt gggacaaggg 3241 cagagatttc gctaccgtgc gcaaagtgct ttctatgcct caagtgaaca tcgtgaagaa 3301 aaccgaggtg caaaccggag gcttttctaa ggaatcaatc ctccccaagc gcaactccga 3361 caagctcatt gcaaggaaga aggattggga ccctaagaag tacggcggat tcgattcacc 3421 aactgtggct tattctgtcc tggtcgtggc taaggtggaa aaaggaaagt ctaagaagct 3481 caagagcgtg aaggaactgc tgggtatcac cattatggag cgcagctcct tcgagaagaa 3541 cccaattgac tttctcgaag ccaaaggtta caaggaagtc aagaaggacc ttatcatcaa 3601 gctcccaaag tatagcctgt tcgaactgga gaatgggcgg aagcggatgc tcgcctccgc 3661 tggcgaactt cagaagggta atgagctggc tctcccctcc aagtacgtga atttcctcta 3721 ccttgcaagc cattacgaga agctgaaggg gagccccgag gacaacgagc aaaagcaact 3781 gtttgtggag cagcataagc attatctgga cgagatcatt gagcagattt ccgagttttc 3841 taaacgcgtc attctcgctg atgccaacct cgataaagtc cttagcgcat acaataagca 3901 cagagacaaa ccaattcggg agcaggctga gaatatcatc cacctgttca ccctcaccaa 3961 tcttggtgcc cctgccgcat tcaagtactt cgacaccacc atcgaccgga aacgctatac 4021 ctccaccaaa gaagtgctgg acgccaccct catccaccag agcatcaccg gactttacga 4081 aactcggatt gacctctcac agctcggagg ggatgaggga gctggatcct gagaccagac 4141 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg 4201 tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc 4261 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca 4321 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa 4381 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca 4441 aaactggtga aactcaccca gggattggct gaaacgaaaa acatattctc aataaaccct 4501 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga 4561 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca 4621 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt 4681 gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga 4741 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg 4801 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc 4861 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta 4921 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg 4981 tgaaagttgg aacctcttac gtgcccgatc aatcatgacc aaaatccctt aacgtgagtt 5041 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 5101 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 5161 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 5221 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt 5281 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 5341 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 5401 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 5461 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 5521 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 5581 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 5641 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 5701 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 5761 ttctgtggat aaccgtag //