LOCUS Exported 6334 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina. ACCESSION . VERSION . KEYWORDS 174_pAAV-ProC39-CatCh-GFP-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6334) AUTHORS Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B TITLE Targeting neuronal and glial cell types with synthetic promoter AAVs in mice, non-human primates and humans. JOURNAL Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 10.1038/s41593-019-0431-2. PUBMED 31285614 REFERENCE 2 (bases 1 to 6334) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6334 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 445..641 /label=SV40 promoter /note="SV40 early promoter" rep_origin 492..627 /label=SV40 ori /note="SV40 origin of replication" primer_bind 554..573 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" regulatory 648..657 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 654..1577 /codon_start=1 /product="N-terminal fragment of channelrhodopsin-2 from the alga Chlamydomonas reinhardtii (Nagel et al., 2003)" /label=ChR2 /note="blue light-activated cation channel" /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI RKTTKLNIGGTEIEVETLVEDEAEAGA" CDS 1584..2300 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1629..1650) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1890..1909) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2237..2258 /label=EGFP-C /note="EGFP, forward primer" misc_feature 2331..2919 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2384..2404) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(2802..2813) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 2951..3427 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(3150..3169) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 3467..3607 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 3467..3596 /label=AAV2 ITR rep_origin 3682..4137 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3769..3788) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3979..4000 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4154..4173) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4273..4295 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4333..4351) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4419..4523 /gene="bla" /label=AmpR promoter CDS 4524..5384 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4742..4761) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5555..6143 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6044..6063 /label=pBR322ori-F /note="pBR322 origin, forward primer" repeat_region 6205..6334 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" ORIGIN 1 gcggccgcac gcgttccaag gcacccttta taaatcaata acgatctaac acctgacgaa 61 ttaattaata acgatccgcg tcgtaggcat aaatcaataa ctgtattact gaatgcgaat 121 taattaataa cgcgattgta gcgcactata aatcaataaa ctggggtttc gtgccgaatt 181 aattaataac gctttaggct aagtggataa atcaataaat atgctctgtt atccgaatta 241 attaataacg gaccatgatg gattcataaa tcaataaggg tcgaaccaga agcgaattaa 301 ttaataacgc cattagctag caagataaat caataatgaa ctactagttg acgaattaat 361 taataacgtg gtcgatgcga tctataaatc aataagtccg ctcgaacaac cgaattaatt 421 aataacggct cgagatctgc gatctgcatc tcaattagtc agcaaccata gtcccgcccc 481 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatcgct 541 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga 601 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aggatccgcc accatggatt 661 atggaggcgc cctgagtgcc gttgggcgcg agctgctatt tgtaacgaac ccagtagtcg 721 tcaatggctc tgtacttgtg cctgaggacc agtgttactg cgcgggctgg attgagtcgc 781 gtggcacaaa cggtgcccaa acggcgtcga acgtgctgca atggcttgct gctggcttct 841 ccatcctact gcttatgttt tacgcctacc aaacatggaa gtcaacctgc ggctgggagg 901 agatctatgt gtgcgctatc gagatggtca aggtgattct cgagttcttc ttcgagttta 961 agaacccgtc catgctgtat ctagccacag gccaccgcgt ccagtggttg cgttacgccg 1021 agtggcttct cacctgcccg gtcatttgca ttcacctgtc aaacctgacg ggcttgtcca 1081 acgactacag caggcgcacc atgggtctgc ttgtgtctga tattggcaca attgtgtggg 1141 gcgccacttc cgccatggcc accggatacg tcaaggtcat cttcttctgc ctgggtctgt 1201 gttatggtgc taacacgttc tttcacgctg ccaaggccta catcgagggt taccacaccg 1261 tgccgaaggg ccggtgtcgc caggtggtga ctggcatggc ttggctcttc ttcgtatcat 1321 ggggtatgtt ccccatcctg ttcatcctcg gccccgaggg cttcggcgtc ctgagcgtgt 1381 acggctccac cgtcggccac accatcattg acctgatgtc gaagaactgc tggggtctgc 1441 tcggccacta cctgcgcgtg ctgatccacg agcatatcct catccacggc gacattcgca 1501 agaccaccaa attgaacatt ggtggcactg agattgaggt cgagacgctg gtggaggacg 1561 aggccgaggc tggcgcggta cccatggtga gcaagggcga ggagctgttc accggggtgg 1621 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg 1681 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca 1741 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca 1801 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct 1861 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg 1921 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg 1981 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata 2041 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg 2101 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc 2161 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca 2221 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg 2281 gcatggacga gctgtacaag tgataagaat tcgatatcaa gcttatcgat aatcaacctc 2341 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc 2401 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca 2461 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg 2521 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca 2581 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg 2641 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg 2701 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctatgttg 2761 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg 2821 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc 2881 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccga gcgctgctcg 2941 agagatctac gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt 3001 tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga 3061 ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt 3121 tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc 3181 acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc 3241 tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg 3301 gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat 3361 ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc 3421 tgtccttctg attttgtagg taaccacgtg cggaccgagc ggccgcagga acccctagtg 3481 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag 3541 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc 3601 ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc 3661 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 3721 tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt 3781 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc 3841 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg 3901 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 3961 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct 4021 cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg 4081 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat 4141 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc 4201 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag 4261 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg 4321 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg 4381 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 4441 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 4501 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct 4561 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag 4621 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta 4681 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc 4741 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca 4801 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg 4861 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg 4921 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca 4981 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa 5041 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa 5101 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 5161 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 5221 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc 5281 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 5341 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 5401 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 5461 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 5521 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 5581 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 5641 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 5701 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 5761 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 5821 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 5881 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 5941 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 6001 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 6061 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 6121 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 6181 tttgctggcc ttttgctcac atgtcctgca ggcagctgcg cgctcgctcg ctcactgagg 6241 ccgcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga 6301 gagggagtgg ccaactccat cactaggggt tcct //