LOCUS       Exported                6257 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    173_pAAV-ProC38-CatCh-GFP-WPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        368..564
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      415..550
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     477..496
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     regulatory      571..580
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             577..1500
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGA"
     CDS             1507..2223
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1552..1573)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1813..1832)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2160..2181
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2254..2842
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2307..2327)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2725..2736)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2874..3350
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3073..3092)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3390..3530
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3390..3519
                     /label=AAV2 ITR
     rep_origin      3605..4060
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3692..3711)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3902..3923
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4077..4096)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4196..4218
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4256..4274)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4342..4446
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4447..5307
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4665..4684)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5478..6066
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5967..5986
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   6128..6257
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 gcggccgcac gcgtcccccc agcttggaca atttgctgac ttgccctatc aaacgcaagc
       61 ttgtgggagt cggcaataat ttgctgactc gcaaccgaac attcaagctt acgcacgcta
      121 tttgaaattt gctgacggct caaggtccca ccaagcttcc attgtgccaa cgaaatttgc
      181 tgacccggat gatgtgactc aagcttccgg tggatccacg gaatttgctg actcagccta
      241 agcagaacaa gcttgcctaa acggtcgtta atttgctgac atctatcctg aacgtcaagc
      301 ttggtcggtt tcgaagaaat ttgctgacat aaaacttgac caacaagctt gctcgagatc
      361 tgcgatctgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
      421 cccctaactc cgcccagttc cgcccattct ccgccccatc gctgactaat tttttttatt
      481 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
      541 tttggaggcc taggcttttg caaatgatcc gccaccatgg attatggagg cgccctgagt
      601 gccgttgggc gcgagctgct atttgtaacg aacccagtag tcgtcaatgg ctctgtactt
      661 gtgcctgagg accagtgtta ctgcgcgggc tggattgagt cgcgtggcac aaacggtgcc
      721 caaacggcgt cgaacgtgct gcaatggctt gctgctggct tctccatcct actgcttatg
      781 ttttacgcct accaaacatg gaagtcaacc tgcggctggg aggagatcta tgtgtgcgct
      841 atcgagatgg tcaaggtgat tctcgagttc ttcttcgagt ttaagaaccc gtccatgctg
      901 tatctagcca caggccaccg cgtccagtgg ttgcgttacg ccgagtggct tctcacctgc
      961 ccggtcattt gcattcacct gtcaaacctg acgggcttgt ccaacgacta cagcaggcgc
     1021 accatgggtc tgcttgtgtc tgatattggc acaattgtgt ggggcgccac ttccgccatg
     1081 gccaccggat acgtcaaggt catcttcttc tgcctgggtc tgtgttatgg tgctaacacg
     1141 ttctttcacg ctgccaaggc ctacatcgag ggttaccaca ccgtgccgaa gggccggtgt
     1201 cgccaggtgg tgactggcat ggcttggctc ttcttcgtat catggggtat gttccccatc
     1261 ctgttcatcc tcggccccga gggcttcggc gtcctgagcg tgtacggctc caccgtcggc
     1321 cacaccatca ttgacctgat gtcgaagaac tgctggggtc tgctcggcca ctacctgcgc
     1381 gtgctgatcc acgagcatat cctcatccac ggcgacattc gcaagaccac caaattgaac
     1441 attggtggca ctgagattga ggtcgagacg ctggtggagg acgaggccga ggctggcgcg
     1501 gtacccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     1561 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     1621 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     1681 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     1741 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     1801 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     1861 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     1921 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     1981 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     2041 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     2101 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     2161 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     2221 aagtgataag aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     2281 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     2341 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     2401 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     2461 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     2521 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     2581 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     2641 tcggggaaat catcgtcctt tccttggctg ctcgcctatg ttgccacctg gattctgcgc
     2701 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     2761 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     2821 tccctttggg ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc tacgggtggc
     2881 atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca
     2941 ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata
     3001 atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta
     3061 gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg
     3121 caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat
     3181 tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca
     3241 ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc
     3301 ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt
     3361 aggtaaccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc
     3421 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     3481 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
     3541 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     3601 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     3661 accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc
     3721 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     3781 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     3841 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     3901 agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat
     3961 ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa
     4021 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
     4081 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     4141 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     4201 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     4261 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     4321 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4381 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4441 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4501 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4561 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4621 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     4681 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     4741 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     4801 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     4861 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     4921 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     4981 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5041 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5101 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5161 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5221 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5281 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5341 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5401 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5461 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5521 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5581 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     5641 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5701 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5761 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5821 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     5881 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5941 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6001 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6061 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6121 cacatgtcct gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg ggcgtcgggc
     6181 gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc
     6241 catcactagg ggttcct
//
