LOCUS Exported 6701 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina. ACCESSION . VERSION . KEYWORDS 168_pAAV-ProC35-CatCh-GFP-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6701) AUTHORS Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B TITLE Targeting neuronal and glial cell types with synthetic promoter AAVs in mice, non-human primates and humans. JOURNAL Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 10.1038/s41593-019-0431-2. PUBMED 31285614 REFERENCE 2 (bases 1 to 6701) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6701 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 679..875 /label=SV40 promoter /note="SV40 early promoter" rep_origin 726..861 /label=SV40 ori /note="SV40 origin of replication" primer_bind 788..807 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" regulatory 882..891 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 888..1811 /codon_start=1 /product="N-terminal fragment of channelrhodopsin-2 from the alga Chlamydomonas reinhardtii (Nagel et al., 2003)" /label=ChR2 /note="blue light-activated cation channel" /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI RKTTKLNIGGTEIEVETLVEDEAEAGA" CDS 1818..2534 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1863..1884) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2124..2143) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2471..2492 /label=EGFP-C /note="EGFP, forward primer" misc_feature 2565..3153 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2618..2638) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3036..3047) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 3185..3661 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(3384..3403) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 3701..3841 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 3701..3830 /label=AAV2 ITR rep_origin 3916..4371 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4003..4022) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4213..4234 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4388..4407) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4507..4529 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4567..4585) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4653..4757 /gene="bla" /label=AmpR promoter CDS 4758..5618 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4976..4995) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5789..6377 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6278..6297 /label=pBR322ori-F /note="pBR322 origin, forward primer" repeat_region 6439..6568 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" ORIGIN 1 cttacatgta cgaagtcggc tagagaccgc ctgaggggat gtcttcacgg gatagcacct 61 gtcacgccca cagctgacgc taattagcgc gaccccacac acaaacagat ggttcattgc 121 cgctgaggca cctgcctcag gtagcaacac gttaactaaa ctaattagct ttgcgcaggt 181 ggcgcggctt cttcctcttc cgtctctttc cttttattaa ggtgtaatca ccacttaatt 241 aataattccg acagttgttc actacatgta tgtcgtggga tgccgaccgc ctgaggggat 301 tccccaggaa ggactcacct gtcgcataca gttgcaaggc taattagcct gccctgagct 361 gccaacagat ggtgtgtctt tttaacctca cctgcctcag gtcaatggcc cctcaataaa 421 ctaattagct ttgcgtcagc cgcagttatt cttcctcttc cgtctctttc ctttttgatt 481 gcacgatcag ccacttaatt aataattgcg tgccgtgtgg catacatgta gctgaggtcc 541 acaggaccgc ctgaggggat cgagatcagc cagcccacct gtcttgcgac ccgatgcggc 601 taattagcac atctaggcgc tgtaacagat ggtcttgtcc cctgcatcca cctgcctcag 661 ggctcgagat ctgcgatctg catctcaatt agtcagcaac catagtcccg cccctaactc 721 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat cgctgactaa 781 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 841 gaggaggctt ttttggaggc ctaggctttt gcaaaggatc cgccaccatg gattatggag 901 gcgccctgag tgccgttggg cgcgagctgc tatttgtaac gaacccagta gtcgtcaatg 961 gctctgtact tgtgcctgag gaccagtgtt actgcgcggg ctggattgag tcgcgtggca 1021 caaacggtgc ccaaacggcg tcgaacgtgc tgcaatggct tgctgctggc ttctccatcc 1081 tactgcttat gttttacgcc taccaaacat ggaagtcaac ctgcggctgg gaggagatct 1141 atgtgtgcgc tatcgagatg gtcaaggtga ttctcgagtt cttcttcgag tttaagaacc 1201 cgtccatgct gtatctagcc acaggccacc gcgtccagtg gttgcgttac gccgagtggc 1261 ttctcacctg cccggtcatt tgcattcacc tgtcaaacct gacgggcttg tccaacgact 1321 acagcaggcg caccatgggt ctgcttgtgt ctgatattgg cacaattgtg tggggcgcca 1381 cttccgccat ggccaccgga tacgtcaagg tcatcttctt ctgcctgggt ctgtgttatg 1441 gtgctaacac gttctttcac gctgccaagg cctacatcga gggttaccac accgtgccga 1501 agggccggtg tcgccaggtg gtgactggca tggcttggct cttcttcgta tcatggggta 1561 tgttccccat cctgttcatc ctcggccccg agggcttcgg cgtcctgagc gtgtacggct 1621 ccaccgtcgg ccacaccatc attgacctga tgtcgaagaa ctgctggggt ctgctcggcc 1681 actacctgcg cgtgctgatc cacgagcata tcctcatcca cggcgacatt cgcaagacca 1741 ccaaattgaa cattggtggc actgagattg aggtcgagac gctggtggag gacgaggccg 1801 aggctggcgc ggtacccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca 1861 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg 1921 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc 1981 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct 2041 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc 2101 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt 2161 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg 2221 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg 2281 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg 2341 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc 2401 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga 2461 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg 2521 acgagctgta caagtgataa gaattcgata tcaagcttat cgataatcaa cctctggatt 2581 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 2641 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 2701 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 2761 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 2821 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 2881 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 2941 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctat gttgccacct 3001 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 3061 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga 3121 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgagcgctg ctcgagagat 3181 ctacgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac 3241 tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt 3301 gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa 3361 gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc 3421 ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga 3481 gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag 3541 acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc 3601 accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct 3661 tctgattttg taggtaacca cgtgcggacc gagcggccgc aggaacccct agtgatggag 3721 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc 3781 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag 3841 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt 3901 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 3961 cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc 4021 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt 4081 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg 4141 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca 4201 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct 4261 attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga 4321 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca 4381 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac 4441 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga 4501 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac 4561 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt 4621 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct 4681 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat 4741 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg 4801 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg 4861 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc 4921 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat 4981 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact 5041 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca 5101 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact 5161 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg 5221 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg 5281 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg 5341 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg 5401 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag 5461 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc 5521 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga 5581 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat 5641 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 5701 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 5761 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 5821 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 5881 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc 5941 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 6001 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 6061 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 6121 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 6181 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 6241 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 6301 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 6361 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 6421 ggccttttgc tcacatgtcc tgcaggcagc tgcgcgctcg ctcgctcact gaggccgccc 6481 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga 6541 gtggccaact ccatcactag gggttcctgc ggccgcacgc gtcttgcttt ggggccttaa 6601 actaattagc tttgagagca ccgacatact tcttcctctt ccgtctcttt ccttttggtg 6661 attacactat cccacttaat taataattta tgtccctgtg t //