LOCUS Exported 6441 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina. ACCESSION . VERSION . KEYWORDS 153_pAAV-ProC31-CatCh-GFP-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6441) AUTHORS Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B TITLE Targeting neuronal and glial cell types with synthetic promoter AAVs in mice, non-human primates and humans. JOURNAL Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 10.1038/s41593-019-0431-2. PUBMED 31285614 REFERENCE 2 (bases 1 to 6441) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6441 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 552..748 /label=SV40 promoter /note="SV40 early promoter" rep_origin 599..734 /label=SV40 ori /note="SV40 origin of replication" primer_bind 661..680 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" regulatory 755..764 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 761..1684 /codon_start=1 /product="N-terminal fragment of channelrhodopsin-2 from the alga Chlamydomonas reinhardtii (Nagel et al., 2003)" /label=ChR2 /note="blue light-activated cation channel" /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI RKTTKLNIGGTEIEVETLVEDEAEAGA" CDS 1691..2407 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1736..1757) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1997..2016) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2344..2365 /label=EGFP-C /note="EGFP, forward primer" misc_feature 2438..3026 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2491..2511) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(2909..2920) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 3058..3534 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(3257..3276) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 3574..3714 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 3574..3703 /label=AAV2 ITR rep_origin 3789..4244 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3876..3895) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4086..4107 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4261..4280) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4380..4402 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4440..4458) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4526..4630 /gene="bla" /label=AmpR promoter CDS 4631..5491 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4849..4868) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5662..6250 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6151..6170 /label=pBR322ori-F /note="pBR322 origin, forward primer" repeat_region 6312..6441 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" ORIGIN 1 gcggccgcac gcgtcggtta atttgcggtg tctgtggttt tggcgaaact tccaggtctg 61 tggtttcgtc acccatccag ggtctgtggt tttcacttga gtcagccgtc tgtggtttac 121 tgcgcaacat tttgtctgtg gtttgacacg ggcccccgtg tctgtggttt cagttgtcag 181 tatttgtctg tggtttaatc ctcgcgcgag cgtctgtggt tttccccgcg gccaacggtc 241 tgtggtttgg ggattgtcat aaagtctgtg gtttgatgga accgctcatg tctgtggttt 301 ttccccctaa agcacgtctg tggtttgtta tacaagagtt tgtctgtggt ttgcgccctg 361 cggaggggtc tgtggtttat cacctacgat cgagtctgtg gtttgaaaca cttatatctg 421 tctgtggttt aatagatgga ttaatgtctg tggttttaga tttcagtcgt ggtctgtggt 481 ttttgcagat ttttactgtc tgtggtttgc cttgccgcga aacgtctgtg gtttgctcga 541 gatctgcgat ctgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 601 cccgccccta actccgccca gttccgccca ttctccgccc catcgctgac taattttttt 661 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg 721 cttttttgga ggcctaggct tttgcaaagg atccgccacc atggattatg gaggcgccct 781 gagtgccgtt gggcgcgagc tgctatttgt aacgaaccca gtagtcgtca atggctctgt 841 acttgtgcct gaggaccagt gttactgcgc gggctggatt gagtcgcgtg gcacaaacgg 901 tgcccaaacg gcgtcgaacg tgctgcaatg gcttgctgct ggcttctcca tcctactgct 961 tatgttttac gcctaccaaa catggaagtc aacctgcggc tgggaggaga tctatgtgtg 1021 cgctatcgag atggtcaagg tgattctcga gttcttcttc gagtttaaga acccgtccat 1081 gctgtatcta gccacaggcc accgcgtcca gtggttgcgt tacgccgagt ggcttctcac 1141 ctgcccggtc atttgcattc acctgtcaaa cctgacgggc ttgtccaacg actacagcag 1201 gcgcaccatg ggtctgcttg tgtctgatat tggcacaatt gtgtggggcg ccacttccgc 1261 catggccacc ggatacgtca aggtcatctt cttctgcctg ggtctgtgtt atggtgctaa 1321 cacgttcttt cacgctgcca aggcctacat cgagggttac cacaccgtgc cgaagggccg 1381 gtgtcgccag gtggtgactg gcatggcttg gctcttcttc gtatcatggg gtatgttccc 1441 catcctgttc atcctcggcc ccgagggctt cggcgtcctg agcgtgtacg gctccaccgt 1501 cggccacacc atcattgacc tgatgtcgaa gaactgctgg ggtctgctcg gccactacct 1561 gcgcgtgctg atccacgagc atatcctcat ccacggcgac attcgcaaga ccaccaaatt 1621 gaacattggt ggcactgaga ttgaggtcga gacgctggtg gaggacgagg ccgaggctgg 1681 cgcggtaccc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt 1741 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga 1801 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc 1861 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga 1921 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg 1981 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg 2041 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat 2101 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa 2161 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt 2221 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc 2281 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga 2341 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct 2401 gtacaagtga taagaattcg atatcaagct tatcgataat caacctctgg attacaaaat 2461 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc 2521 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt 2581 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg 2641 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg 2701 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc 2761 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt 2821 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tatgttgcca cctggattct 2881 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg 2941 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg 3001 gatctccctt tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga gatctacggg 3061 tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg 3121 cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc 3181 tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc 3241 tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc 3301 actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg 3361 ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt 3421 ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg 3481 cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt 3541 ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca 3601 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc 3661 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc 3721 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca 3781 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 3841 cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt 3901 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 3961 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg 4021 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 4081 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt 4141 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca 4201 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag 4261 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga 4321 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc 4381 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg 4441 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc 4501 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 4561 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 4621 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 4681 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 4741 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 4801 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 4861 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 4921 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 4981 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 5041 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 5101 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 5161 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 5221 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 5281 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 5341 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 5401 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 5461 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 5521 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 5581 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 5641 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 5701 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 5761 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta 5821 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 5881 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 5941 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 6001 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 6061 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 6121 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 6181 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 6241 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 6301 tgctcacatg tcctgcaggc agctgcgcgc tcgctcgctc actgaggccg cccgggcgtc 6361 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 6421 actccatcac taggggttcc t //