LOCUS Exported 5808 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses VfAU1-LOV domain (Vaucheria frigida) followed by restriction sites. ACCESSION . VERSION . KEYWORDS pOVC1_VfAU1-LOV_X SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5808) AUTHORS Tichy AM, Gerrard EJ, Legrand JMD, Hobbs RM, Janovjak H TITLE Engineering Strategy and Vector Library for the Rapid Generation of Modular Light-Controlled Protein-Protein Interactions. JOURNAL J Mol Biol. 2019 May 29. pii: S0022-2836(19)30317-1. doi: 10.1016/j.jmb.2019.05.033. PUBMED 31150735 REFERENCE 2 (bases 1 to 5808) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5808 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 186..565 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 566..769 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 720..740 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 814..833 /label=T7 /note="T7 promoter, forward primer" promoter 814..832 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1350..1367) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 1356..1580 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 1626..2054 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1713..1732) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 1923..1944 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(2063..2083) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 2068..2397 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 2248..2383 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2310..2329 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2464..3258 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2518..2537) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3128..3147 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 3432..3553 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3469..3488) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3523..3542 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(3602..3618) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3602..3618) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3615..3637) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 3626..3642 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3650..3680) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3695..3716 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(3833..3850) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4004..4592) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4084..4103) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4763..5623) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5386..5405 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5624..5728) /gene="bla" /label=AmpR promoter primer_bind complement(5803..14) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc 61 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat 121 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac 181 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca 241 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc 301 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat 361 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt 421 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 481 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 541 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg 601 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt 661 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac 721 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac 781 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca 841 agctgaccat ggccaccggt cctgactaca gtctcgtgaa ggctctgcaa atggcacaac 901 agaattttgt cattacagac gcctccctcc cagacaaccc tatcgtctac gccagtagag 961 ggtttctgac actgacaggc tattctctcg accagatcct gggcaggaac tgcaggtttc 1021 tgcaagggcc agaaacagac ccaagagctg tggataagat caggaatgcc atcaccaaag 1081 gcgttgatac cagtgtctgt ctgctgaatt atagacagga tggcacaacc ttctggaatc 1141 tcttcttcgt ggctggactc agagattcta agggcaatat tgtcaactac gtcggagtgc 1201 agtcaaaggt gagcgaagat tatgccaagc tgctggtcaa cgagcagaac attgagtaca 1261 aaggtgtgcg caccagtaac atgctgcgca gaaagcccgg tgaattctca ggctctggat 1321 cccccgggta agtttaaacc gctgatcagc ctcgactgtg ccttctagtt gccagccatc 1381 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct 1441 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg 1501 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg 1561 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta 1621 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 1681 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 1741 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 1801 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 1861 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 1921 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 1981 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 2041 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 2101 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 2161 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 2221 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 2281 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc 2341 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag 2401 ctctcgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt 2461 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta 2521 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg 2581 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa 2641 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct 2701 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg 2761 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca 2821 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat 2881 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac 2941 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc 3001 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa 3061 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag 3121 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc 3181 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt 3241 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca 3301 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa 3361 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct 3421 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 3481 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 3541 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat 3601 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 3661 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 3721 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 3781 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 3841 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 3901 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 3961 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 4021 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 4081 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 4141 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 4201 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 4261 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 4321 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 4381 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 4441 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 4501 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 4561 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 4621 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 4681 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 4741 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 4801 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 4861 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 4921 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 4981 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 5041 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 5101 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 5161 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 5221 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 5281 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 5341 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 5401 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 5461 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 5521 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 5581 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 5641 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 5701 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg 5761 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaat //