LOCUS Exported 5904 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Expresses RsLP-LOV (Rhodobacter sphaeroides) preceded by restriction sites. ACCESSION . VERSION . KEYWORDS pOVC1_X_RsLP-LOV SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5904) AUTHORS Tichy AM, Gerrard EJ, Legrand JMD, Hobbs RM, Janovjak H TITLE Engineering Strategy and Vector Library for the Rapid Generation of Modular Light-Controlled Protein-Protein Interactions. JOURNAL J Mol Biol. 2019 May 29. pii: S0022-2836(19)30317-1. doi: 10.1016/j.jmb.2019.05.033. PUBMED 31150735 REFERENCE 2 (bases 1 to 5904) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5904 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 158..537 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 538..741 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 692..712 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 786..805 /label=T7 /note="T7 promoter, forward primer" promoter 786..804 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1418..1435) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 1424..1648 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 1694..2122 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1781..1800) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 1991..2012 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(2131..2151) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 2136..2465 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 2316..2451 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2378..2397 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2532..3326 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2586..2605) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3196..3215 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 3500..3621 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3537..3556) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3591..3610 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(3670..3686) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3670..3686) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3683..3705) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 3694..3710 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3718..3748) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3763..3784 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(3901..3918) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4072..4660) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4152..4171) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4831..5691) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5454..5473 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5692..5796) /gene="bla" /label=AmpR promoter primer_bind complement(5871..5890) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct 61 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt 121 gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat 181 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca 241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca 301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg 361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg 421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc 481 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg 541 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca 601 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt 661 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg 721 gaggtctata taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc 781 gaaattaata cgactcacta tagggagacc caagctgacc atggccaccg gtgaattctc 841 aggctctgga tcccccggtg ccatggatca gaagcagttt gagaagatta gagctgtgtt 901 tgacaggtca ggggtcgcac tgaccctcgt tgacatgtcc ctgccagagc aacccctggt 961 gctcgccaac cctccatttc tgagaatgac tggctatact gagggccaga tcctgggatt 1021 caactgcaga tttctccaga gaggcgacga aaatgctcag gcacgggctg acatcagaga 1081 tgccctcaag ctcggaaggg agctccaggt ggtcctccgc aattacagag ccaacgatga 1141 accatttgac aatctgctgt tcctgcaccc tgtcggtggc agacccgacg ctcctgacta 1201 cttcctcggt tctcagttcg agctgggtag aagcggaaat agcgaagagg cagccgcagc 1261 tggacacgca ggggcactga ctggggagct cgccagaata ggaactgtgg ctgctcggct 1321 cgaaatggac agtcggagac atctggcaca agctgctgca gccctggtga gggcctggga 1381 aagaaggggt cccgggtaag tttaaaccgc tgatcagcct cgactgtgcc ttctagttgc 1441 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc 1501 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct 1561 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg 1621 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct 1681 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 1741 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 1801 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta 1861 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 1921 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg 1981 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat 2041 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt 2101 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt 2161 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 2221 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 2281 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 2341 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 2401 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt 2461 gcaaaaagct ctcgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag 2521 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg 2581 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt 2641 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc 2701 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt 2761 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag 2821 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg 2881 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag 2941 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg 3001 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc 3061 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca 3121 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc 3181 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg 3241 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct 3301 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc 3361 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg 3421 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct 3481 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa 3541 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 3601 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc 3661 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 3721 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 3781 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 3841 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 3901 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 3961 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 4021 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 4081 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 4141 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 4201 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 4261 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 4321 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 4381 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 4441 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 4501 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 4561 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 4621 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 4681 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 4741 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 4801 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 4861 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 4921 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 4981 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 5041 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 5101 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 5161 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 5221 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 5281 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 5341 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 5401 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 5461 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 5521 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 5581 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 5641 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 5701 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 5761 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 5821 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc 5881 tctgatgccg catagttaag ccag //