LOCUS       Exported                6150 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    206_pAAV-ProD17-CatCh-GFP-WPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   25..154
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        533..564
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      618..627
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             624..1547
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGA"
     CDS             1554..2270
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1599..1620)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1860..1879)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2207..2228
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2301..2889
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2354..2374)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2772..2783)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2921..3397
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3120..3139)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3437..3577
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3437..3566
                     /label=AAV2 ITR
     rep_origin      3652..4107
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3739..3758)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3949..3970
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4124..4143)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4243..4265
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4303..4321)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4389..4493
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4494..5354
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4712..4731)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5525..6113
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6014..6033
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttgctggcc ttttgctcac atgtcctgca ggcagctgcg cgctcgctcg ctcactgagg
       61 ccgcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga
      121 gagggagtgg ccaactccat cactaggggt tcctgcggcc gcacgcgtat cctggggttt
      181 aaacaggact aggtccactg ggagaagagg aagtcaaaca ggactagtca tcgataggct
      241 agccagctta attaatcaga tactctaggg ctgtgagtga aaggtcggcg aaagttggag
      301 ggcttggctt ggaactggca acactgtgct cattactgtc cgggtaatga ggggacgatg
      361 ctcattacgg ttggtttctt ggctacatgc tcaggaatcc tcaacttcct cctgttcagt
      421 tcccagcaag tgctgggcat tgtgtcagac aagtcccgta tagtccgcca tttatcctgt
      481 aagaggcatc tgaggcacac gtcatatcct cacatggtcc tgctggagtt agtagagggt
      541 atataatgga agctcgactt ccagctatca catccactgt gttgttgtga actggaatcc
      601 actataggcc aggatccgcc accatggatt atggaggcgc cctgagtgcc gttgggcgcg
      661 agctgctatt tgtaacgaac ccagtagtcg tcaatggctc tgtacttgtg cctgaggacc
      721 agtgttactg cgcgggctgg attgagtcgc gtggcacaaa cggtgcccaa acggcgtcga
      781 acgtgctgca atggcttgct gctggcttct ccatcctact gcttatgttt tacgcctacc
      841 aaacatggaa gtcaacctgc ggctgggagg agatctatgt gtgcgctatc gagatggtca
      901 aggtgattct cgagttcttc ttcgagttta agaacccgtc catgctgtat ctagccacag
      961 gccaccgcgt ccagtggttg cgttacgccg agtggcttct cacctgcccg gtcatttgca
     1021 ttcacctgtc aaacctgacg ggcttgtcca acgactacag caggcgcacc atgggtctgc
     1081 ttgtgtctga tattggcaca attgtgtggg gcgccacttc cgccatggcc accggatacg
     1141 tcaaggtcat cttcttctgc ctgggtctgt gttatggtgc taacacgttc tttcacgctg
     1201 ccaaggccta catcgagggt taccacaccg tgccgaaggg ccggtgtcgc caggtggtga
     1261 ctggcatggc ttggctcttc ttcgtatcat ggggtatgtt ccccatcctg ttcatcctcg
     1321 gccccgaggg cttcggcgtc ctgagcgtgt acggctccac cgtcggccac accatcattg
     1381 acctgatgtc gaagaactgc tggggtctgc tcggccacta cctgcgcgtg ctgatccacg
     1441 agcatatcct catccacggc gacattcgca agaccaccaa attgaacatt ggtggcactg
     1501 agattgaggt cgagacgctg gtggaggacg aggccgaggc tggcgcggta cccatggtga
     1561 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     1621 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     1681 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     1741 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     1801 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     1861 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     1921 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     1981 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     2041 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     2101 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     2161 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     2221 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag tgataagaat
     2281 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     2341 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     2401 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     2461 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     2521 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     2581 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     2641 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     2701 cgtcctttcc ttggctgctc gcctatgttg ccacctggat tctgcgcggg acgtccttct
     2761 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     2821 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     2881 cctccccgca tcgataccga gcgctgctcg agagatctac gggtggcatc cctgtgaccc
     2941 ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct
     3001 aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg
     3061 gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt
     3121 ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc
     3181 ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat
     3241 gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag
     3301 gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg
     3361 attacaggcg tgaaccactg ctcccttccc tgtccttctg attttgtagg taaccacgtg
     3421 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     3481 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     3541 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
     3601 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
     3661 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     3721 ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     3781 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     3841 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     3901 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     3961 tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta taagggattt
     4021 tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4081 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
     4141 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     4201 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     4261 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     4321 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     4381 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4441 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4501 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4561 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4621 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4681 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4741 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4801 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4861 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     4921 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     4981 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5041 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5101 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5161 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5221 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5281 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5341 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5401 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5461 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5521 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5581 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5641 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     5701 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5761 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5821 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5881 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5941 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6001 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6061 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6121 gcaacgcggc ctttttacgg ttcctggcct
//
