LOCUS       Exported                6408 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    180_pAAV-ProD11-CatCh-GFP-WPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6408)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6408
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        677..708
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      762..771
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             768..1691
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGA"
     CDS             1698..2414
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1743..1764)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2004..2023)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2351..2372
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2445..3033
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2498..2518)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2916..2927)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3065..3541
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3264..3283)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3581..3721
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3581..3710
                     /label=AAV2 ITR
     rep_origin      3796..4251
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3883..3902)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4093..4114
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4268..4287)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4387..4409
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4447..4465)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4533..4637
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4638..5498
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4856..4875)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5669..6257
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6158..6177
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   6319..40
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 cgcagagagg gagtggccaa ctccatcact aggggttcct gcggccgcac gcgtatcctg
       61 gggtttaaac aggactaggt ccactgggag aagaggaagt caaacaggac tagtcatcga
      121 taggctagcc agcttaatta atcagatgca ggagtttgag ggatgcggat gaataggttg
      181 agggttgagg accactgcgc ctctgatggt tgtctagagg cagacaatac gcagtctgga
      241 gctggagcag gtgcgtgcag tgtgcattgg ggcttctgcc agtgatccaa gataggaaga
      301 catagtgagt gcttagaaga gcaccagcat gtgaagggct gagaagtggt cggcgaggaa
      361 aggccctcaa aggagcctca gagggagtgg acacagagag gcttaaagtt aagagcatcg
      421 agaagacagc tcttcgtgga aggggtgtgg cctccagtta ggttgttggg gaattgtaga
      481 gataaatgaa agtatccgtt agacttgcaa aggcgatgac aaaaataaac ctggttaatg
      541 ctgtcagtgg tttgaaagtg agcaagaaga ttgcaccaga ttaaagagca agtgagcagt
      601 ctgttaaatg tacatgtctg ttggtaggag catggccacc cttgaagcaa tcctcacatg
      661 gtcctgctgg agttagtaga gggtatataa tggaagctcg acttccagct atcacatcca
      721 ctgtgttgtt gtgaactgga atccactata ggccaggatc cgccaccatg gattatggag
      781 gcgccctgag tgccgttggg cgcgagctgc tatttgtaac gaacccagta gtcgtcaatg
      841 gctctgtact tgtgcctgag gaccagtgtt actgcgcggg ctggattgag tcgcgtggca
      901 caaacggtgc ccaaacggcg tcgaacgtgc tgcaatggct tgctgctggc ttctccatcc
      961 tactgcttat gttttacgcc taccaaacat ggaagtcaac ctgcggctgg gaggagatct
     1021 atgtgtgcgc tatcgagatg gtcaaggtga ttctcgagtt cttcttcgag tttaagaacc
     1081 cgtccatgct gtatctagcc acaggccacc gcgtccagtg gttgcgttac gccgagtggc
     1141 ttctcacctg cccggtcatt tgcattcacc tgtcaaacct gacgggcttg tccaacgact
     1201 acagcaggcg caccatgggt ctgcttgtgt ctgatattgg cacaattgtg tggggcgcca
     1261 cttccgccat ggccaccgga tacgtcaagg tcatcttctt ctgcctgggt ctgtgttatg
     1321 gtgctaacac gttctttcac gctgccaagg cctacatcga gggttaccac accgtgccga
     1381 agggccggtg tcgccaggtg gtgactggca tggcttggct cttcttcgta tcatggggta
     1441 tgttccccat cctgttcatc ctcggccccg agggcttcgg cgtcctgagc gtgtacggct
     1501 ccaccgtcgg ccacaccatc attgacctga tgtcgaagaa ctgctggggt ctgctcggcc
     1561 actacctgcg cgtgctgatc cacgagcata tcctcatcca cggcgacatt cgcaagacca
     1621 ccaaattgaa cattggtggc actgagattg aggtcgagac gctggtggag gacgaggccg
     1681 aggctggcgc ggtacccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     1741 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     1801 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     1861 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     1921 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     1981 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     2041 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     2101 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     2161 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     2221 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     2281 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     2341 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     2401 acgagctgta caagtgataa gaattcgata tcaagcttat cgataatcaa cctctggatt
     2461 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     2521 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     2581 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     2641 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     2701 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     2761 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     2821 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctat gttgccacct
     2881 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     2941 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     3001 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgagcgctg ctcgagagat
     3061 ctacgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac
     3121 tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt
     3181 gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa
     3241 gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc
     3301 ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga
     3361 gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag
     3421 acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc
     3481 accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct
     3541 tctgattttg taggtaacca cgtgcggacc gagcggccgc aggaacccct agtgatggag
     3601 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
     3661 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag
     3721 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
     3781 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     3841 cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc
     3901 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     3961 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     4021 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     4081 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct
     4141 attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga
     4201 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
     4261 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     4321 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     4381 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     4441 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     4501 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     4561 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     4621 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     4681 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     4741 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     4801 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     4861 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     4921 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     4981 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5041 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5101 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     5161 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     5221 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     5281 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     5341 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     5401 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     5461 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     5521 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     5581 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     5641 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5701 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5761 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     5821 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5881 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5941 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6001 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6061 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6121 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6181 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6241 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6301 ggccttttgc tcacatgtcc tgcaggcagc tgcgcgctcg ctcgctcact gaggccgccc
     6361 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcg
//
