LOCUS       Exported               13200 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  For knocking in TREX1 in mouse - Doxycyclin inducible.
ACCESSION   .
VERSION     .
KEYWORDS    pTRIPZ TREX1-KI
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13200)
  AUTHORS   Vanpouille-Box C, Alard A, Aryankalayil MJ, Sarfraz Y, Diamond JM, 
            Schneider RJ, Inghirami G, Coleman CN, Formenti SC, Demaria S
  TITLE     DNA exonuclease Trex1 regulates radiotherapy-induced tumour 
            immunogenicity.
  JOURNAL   Nat Commun. 2017 Jun 9;8:15618. doi: 10.1038/ncomms15618.
  PUBMED    28598415
REFERENCE   2  (bases 1 to 13200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13200
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    2722..2740
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2757..2775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2793..2811
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2829..2847
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2864..2882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2900..2918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2930..2968
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2965..2989
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        3950..5161
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4998..5017
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    5174..5207
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACAT). loxP511 sites are compatible with each
                     other, but incompatible with wild-type loxP sites."
     CDS             5214..5921
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     protein_bind    5922..5955
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACAT). loxP511 sites are compatible with each
                     other, but incompatible with wild-type loxP sites."
     misc_feature    5978..6550
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6145..6162)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6372..6391
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6553..7155
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7052..7072
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7171..7759
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7224..7244)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7642..7653)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7966..8199
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8300..8317)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8306..8530
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8576..9004
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8663..8682)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8873..8894
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9013..9033)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9018..9347
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9198..9333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9260..9279
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    10555..10676
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10725..10741)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10725..10741)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10738..10760)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10749..10765
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10773..10803)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10818..10839
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10956..10973)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11127..11715)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11207..11226)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11886..12746)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12509..12528
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12747..12851)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    12899..13033
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gacgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc
     2761 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
     2821 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga
     2881 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt
     2941 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg caccggtcat
     3001 gggctcacag accctgcccc atggtcacat gcagaccctc atcttcttag acctggaagc
     3061 cactggcctg ccttcgtctc ggcccgaagt cacagagctg tgcctgctgg ctgtccacag
     3121 acgtgctctg gagaacactt ccatttctca gggacatcca cctccagtgc ccagaccgcc
     3181 ccgtgtggtg gacaagctct ctctgtgcat tgctccaggg aaagcctgta gccctggggc
     3241 cagtgagatc acaggtctga gcaaagctga gctggaagta caggggcgtc aacgcttcga
     3301 tgacaacctg gccatcctgc tccgagcctt cctgcagcgc cagccacagc cttgctgcct
     3361 tgtggcacac aacggtgacc gctatgactt tcctctgctc cagacagagc ttgctaggct
     3421 gagcactccc agtcccctag atggtacctt ctgtgtggac agcatcgctg ccctaaaggc
     3481 cttggaacaa gctagcagcc cctcagggaa tggttcgagg aaaagctaca gcctgggcag
     3541 catctacacc cgcctgtact ggcaagcacc gacagactca catactgctg aaggtgatgt
     3601 tctaaccctg ctcagcatct gtcagtggaa gccacaggcc ctactgcagt gggtggacga
     3661 acatgcccgg ccctttagca ccgtcaagcc catgtacggc actccggcta ccactggaac
     3721 aaccaaccta aggccacatg ctgccacagc tactacaccc ctggccacag ccaatggaag
     3781 tcccagcaat ggcaggagca ggcgacctaa gagtcctcct ccagagaagg tcccagaagc
     3841 cccatcacag gaggggctgc tggccccact gagcctgctg accctcctga ccttggcaat
     3901 agccactctg tatggactct tcctggcctc acctgggcag taaacgcgtg gcctccgcgc
     3961 cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac
     4021 gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc ggcccgctgc
     4081 tcataagact cggccttaga accccagtat cagcagaagg acattttagg acgggacttg
     4141 ggtgactcta gggcactggt tttctttcca gagagcggaa caggcgagga aaagtagtcc
     4201 cttctcggcg attctgcgga gggatctccg tggggcggtg aacgccgatg attatataag
     4261 gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgggtc gcggttcttg
     4321 tttgtggatc gctgtgatcg tcacttggtg agtagcgggc tgctgggctg gccggggctt
     4381 tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta
     4441 gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg
     4501 cggctgttcc cgagtcttga atggaagacg cttgtgaggc gggctgtgag gtcgttgaaa
     4561 caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg
     4621 gaaagctctt attcgggtga gatgggctgg ggcaccatct ggggaccctg acgtgaagtt
     4681 tgtcactgac tggagaactc ggtttgtcgt ctgttgcggg ggcggcagtt atggcggtgc
     4741 cgttgggcag tgcacccgta cctttgggag cgcgcgccct cgtcgtgtcg tgacgtcacc
     4801 cgttctgttg gcttataatg cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt
     4861 tctccgtcgc aggacgcagg gttcgggcct agggtaggct ctcctgaatc gacaggcgcc
     4921 ggacctctgg tgaggggagg gataagtgag gcgtcagttt ctttggtcgg ttttatgtac
     4981 ctatcttctt aagtagctga agctccggtt ttgaactatg cgctcggggt tggcgagtgt
     5041 gttttgtgaa gttttttagg caccttttga aatgtaatca tttgggtcaa tatgtaattt
     5101 tcagtgttag actagtaaat tgtccgctaa attctggccg tttttggctt ttttgttaga
     5161 cgctagcgga tccataactt cgtatagtat acattatacg aagttatgcc accatgtcta
     5221 ggctggacaa gagcaaagtc ataaacggag ctctggaatt actcaatggt gtcggtatcg
     5281 aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact
     5341 ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc
     5401 atcataccca cttctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg
     5461 ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca
     5521 cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc
     5581 aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg
     5641 gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca
     5701 ccgattctat gcccccactt ctgagacaag caattgagct gttcgaccgg cagggagccg
     5761 aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt
     5821 gcgaaagcgg cgggccgacc gacgcccttg acgattttga cttagacatg ctcccagccg
     5881 atgcccttga cgattttgac cttgacatgc tccccgggta aataacttcg tatagtatac
     5941 attatacgaa gttatggatc cgcggccgca aattccgccc ctctccctcc ccccccccta
     6001 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     6061 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     6121 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     6181 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     6241 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     6301 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     6361 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     6421 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     6481 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     6541 cacgataata ccatggccac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     6601 gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     6661 accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     6721 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     6781 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg ctcgcgcatg
     6841 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     6901 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     6961 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgctggg
     7021 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     7081 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     7141 aagcccggtg cctgagttcg cgtctggaac aatcaacctc tggattacaa aatttgtgaa
     7201 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     7261 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     7321 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     7381 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     7441 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     7501 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     7561 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     7621 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     7681 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     7741 ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag
     7801 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
     7861 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca
     7921 aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc
     7981 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     8041 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8101 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8161 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     8221 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt
     8281 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     8341 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     8401 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     8461 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     8521 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     8581 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     8641 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     8701 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     8761 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     8821 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     8881 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     8941 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     9001 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     9061 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     9121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     9181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     9241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     9301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     9361 cttgtatatc cattttcgga tctgatcagc acgtgatgaa aaagcctgaa ctcaccgcga
     9421 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct
     9481 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
     9541 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
     9601 cggccgcgct cccgattccg gaagtgcttg acattgggga attaattcag cgagagcctg
     9661 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
     9721 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt
     9781 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     9841 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     9901 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     9961 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
    10021 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
    10081 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
    10141 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc
    10201 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
    10261 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
    10321 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
    10381 agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagcacgt gctacgagat
    10441 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
    10501 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
    10561 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    10621 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    10681 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
    10741 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
    10801 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
    10861 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    10921 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    10981 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    11041 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    11101 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    11161 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    11221 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    11281 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    11341 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    11401 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    11461 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    11521 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    11581 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    11641 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    11701 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    11761 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    11821 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    11881 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    11941 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    12001 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    12061 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    12121 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    12181 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    12241 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    12301 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    12361 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    12421 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    12481 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    12541 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    12601 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    12661 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    12721 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    12781 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    12841 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa
    12901 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    12961 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    13021 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
    13081 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc
    13141 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
